Metabolic engineering is essential for the development of microbial cell factories to produce biomolecules from low-value renewable substrates. This role helps advance the development of ecologically responsible and commercially robust chemical industries, including biofuels and high-value compounds like medicines. The ability of microbial cell factories to generate a wide variety of substances sustainably, therefore satisfying modern commodity needs, has piqued the scientific community's attention. The goal of metabolic engineering is to convert different microorganisms into efficient cell factories to produce desired products, and it has been used for decades to develop novel metabolic pathways and alter pre-existing ones with the help of system biology, synthetic biology, and evolutionary engineering.