Efficient thin film composite polyamide (PA) membranes require optimization of interfacial polymerization (IP) process. However, it is challengeable because of its ultrafast reaction rate coupling with mass and heat transfer, resulting in PA membranes with low performance. Here, we propose a non-isothermal-controlled IP strategy to fabricate highly permeable and selective PA membrane by engineering the IP process at cryogenic aqueous phase (CAP). In this strategy, CAP allows the phase transition of aqueous solution from liquid to solid state, which not only achieves synchronous control of heat and mass transfer in interfacial region but also enriches understanding of the mechanisms involved different phase state on IP process. Our PA membrane exhibits excellent separation performance with ultrahigh water permeance (42.9 L m-2 h-1 bar-1) and antibiotic desalination efficiency (antibiotic/NaCl selectivity of 159.3). Our study provides a new dimension for deep understanding of exact mechanism linking the IP to the targeting membrane performance.