Objective: The rapid palatal expansion (RPE) procedure is a frequently used treatment for malocclusion before pubertal growth. However, relapses often occur after the retention period of this procedure. In this study, we aimed to eliminate this deficiency, considering that there would be a deficiency in the new bone metabolism in the expanded area between the palatal sutures. Design: Twenty-four male Wistar rats, 7-8 weeks old, with an average weight of 200 g, were divided into 3 groups in equal numbers. 5 days of expansion was applied to all groups with 50 g of force, then a retention period of 12 days was expected. While no application was made to the control group (CG) during the retention period, mechanical vibration was applied to the vibration group (VF) at a frequency of 111 Hz and the active substance was applied to the thymoquinone group (TQ) at a dose of 10 mg/kg. Result: At the end of the retention period, all animals were sacrificed and sections were taken from the premaxillary region and histological evaluation was made in terms of osteoblast, osteoclast, capillary numbers, amount of mineralized and fibrous areas. According to the statistical analysis, TQ and VF showed more positive and similar results than CG in terms of new bone formation for all histomorphometric parameters except osteoclast numbers in the comparison of parameters in all groups (p<0.05). Conclusions: The new bone stimulation effects of non-invasive vibration force and Thymoquinone applications and ease of use can prevent relapse in the RPE.