It has been over 200 years since the organ/tissue perfusion was first used in the experimental research. Today organ/tissue perfusion has been widely used in the study of pathological mechanisms study, drug metabolism, drugs screening, tissues/organs fixation, cells preparation, cancer topical treatment, target products preparation, experimental methods improvement and makes great contribution to the scientific researches. In the present paper, we provide a comprehensive overview of experimental organ/tissue perfusion, mainly covering the classification, experimental influence factors and their application.