In this paper, we consider the non-fragile containment control problem of nonlinear multi-agent systems (MASs) with exogenous disturbance where the communication links among agents under consideration is directed. Firstly, based on relative output measurements between the agent and its neighbors, a disturbance observer-based control protocol is proposed to solve the containment control problem of MASs with inherent nonlinear dynamics and exogenous disturbances. Secondly, because of the additional tuning of parameters in the real control systems, uncertainties in the designing of observer and controller gains always occur, and as a result, an output feedback controller with disturbance rejection is conceived and the containment control problem of nonlinear MASs with non-fragility is thoroughly investigated. Then, depending on matrix transformation and inequality technique, sufficient conditions of the designed controller gains exist, which is derived from the asymptotic stability analysis problem of some containment error dynamics of MASs. Finally, two simulation examples are exploited to illustrate the effectiveness of the proposed techniques.