Rationale: Many insect species undertake multi-generational migrations in the Afro-tropical and Palearctic ranges, and understanding their migratory connectivity remains challenging due to their small size, short life span and large population sizes. Hydrogen isotope ( δ 2H) can be used to reconstruct the movement of dispersing or migrating insects, but applying δ 2H for provenance requires a robust isotope baseline map (i.e., isoscape) for the Afro-Palearctic. Methods: We analysed the δ 2H in the wings ( δ 2H wing) of 142 resident butterflies from 56 sites across the Afro-Palearctic. The δ 2H wing values were compared to the predicted local growing-season precipitation δ 2H values ( δ 2H GSP) using a linear regression model to develop an insect wing δ 2H isoscape. We used multivariate linear mixed models and high-resolution and time-specific remote sensing climate and environmental data to explore the controls of the residual δ 2H wing variability. Results: A strong linear relationship was found between δ 2H wing and δ 2H GSP values (r 2=0.53). The resulting isoscape showed strong patterns across the Palearctic but limited variation and high uncertainty for the Afro-tropics. Positive residuals of this relationship were correlated with dry conditions for the month preceding sampling whereas negative residuals were correlated with more wet days for the month preceding sampling. High intra-site δ 2H wing variance was associated with lower relative humidity for the month preceding sampling and higher elevation. Conclusion: The δ 2H wing isoscape is applicable to trace butterflies, moths and other terrestrial herbivorous insects that migrate across the Afro-Palearctic range but has limited geolocation potential in the Afro-tropics. The spatial analysis of uncertainty using high-resolution climatic data demonstrated that many African regions with highly variable evaporation rates and relative humidity have δ 2H wing values that are less related to δ 2H GSP values. Increasing geolocation precision will require new modeling approaches using more time-specific environmental data and/or independent geolocation tools.

There is a growing need to address water security issues in Canada in relation to global warming effects and water management issues. Stable water isotopes (𝛿2H and 𝛿18O) have become a popular tool to investigate regional and country level public water distribution systems to identify regional water resource issues and their underlying causes. In this study, we built the first national level maps of isotopic values in tap water across Canada using 576 tap water samples that were collected during the summer season. Observed isotopic values in tap water follow similar patterns as precipitation with highest isotopic values in the coastal and Great Lakes regions and a progressive decrease as continentality increases with lowest values found in the Rocky Mountains. We classified the tap water samples based on their supply sources including groundwater, river and lake. The isotopic values of tap water sourced from groundwater and river are well-predicted by those of local annual precipitation whereas those sourced from lakes are poorly predicted. To explain this difference, we constructed a series of water balance models to predict the 𝛿2H and 𝛿18O variability of surface water across Canada. We used a digital elevation model to accumulate the isotopic composition of local precipitation weighed by precipitation (total or effective) either monthly, seasonally or annually. These water balance models strongly improved the predictability of isotopic values of tap waters sourced from rivers and lakes (particularly the annual average and monthly weighted summer models, with or without accounting for evapotranspiration). Conversely, these models did not improve predictability of the isotopic values in tap water sourced from groundwater. We argue that isotope values of tap water sourced from surface water reflect the accumulation and evapotranspiration of precipitation in catchments whereas groundwater represents an annual average of the isotopic composition of local precipitation. Regionally, we find that winter precipitation and snow melt and glaciers melt from the Rockies contribute to a large part of tap water sources around the Rockies making them vulnerable to water balance changes with ongoing climate warming. In eastern Canada water management processes appear to create some significant evaporative water losses whereas in central Canada dry continental climate causes significant evaporative water loss. Our study highlights the cycling of water from precipitation to tap water across Canada for different tap water supply with implications for water managements, contamination risks and vulnerability to climate change. Our models and databases also provide an isotopic baseline for regionally important water resources monitoring as well as for human forensic cases.