The hydrogen isotopic compositions of the leaf-wax n-alkanes (δDalk) and n-alkanoic acids (δDacid) are known to reflect ambient climatic conditions (including precipitation δD values, δDPrecip). However, the climatic conditions of exactly which period (i.e. early or entire period of the leaf’s lifespan) these biomarkers represent, i.e. the seasonality in δDalk and δDacid records, is still evolving. The seasonality studies on the δDalk and δDacid values, done only in extra-tropical regions, mostly indicate the δDalk values are biased towards the early growing season whereas δDacid values are not biased towards any season. To decipher the seasonality in the δDalk and δDacid records from the tropics, we conducted a long-duration experiment wherein deciduous and evergreen species were grown using normal water (δD = −2‰) during the early stages of the leaf's growth and later using isotopically-labeled water (δD = 1000‰). Our experiment revealed (i) in deciduous and evergreen species, δDalk and δDacid values reflect δDPrecip during the early stages of the leaf's growth, (ii) synchronous synthesis of n-alkanes and n-alkanoic acids, and (iii) in deciduous species, minor incorporation of the previous year’s photosynthates in the leaf wax pool of the current year’s mature leaves. Our study suggests that the δDalk and δDacid records in the tropics are biased towards the climatic conditions prevailing during the early stages of the leaf's growth. This bias should be considered while comparing the δDPrecip values generated from the leaf wax proxy records and isotope-enabled atmospheric circulation models.

A document by Chaithanya B P. Click on the document to view its contents.

Climate change, inter-annual precipitation variability, recurrent droughts, and flash flooding, coupled with increasing water needs, are shaping the co-evolution of socioeconomic and cultural assemblages, water laws and regulations, and equitable drinking water access and allocation worldwide. Recognizing the need for mitigation strategies for drinking water availability in urban areas, the Isotope Hydrology Section of the International Atomic Energy Agency (IAEA) coordinated a state-of-the-art global assessment to evaluate water sources and distribution of drinking water supply in urban centers, an initiative entitled “Use of Isotope Techniques for the Evaluation of Water Sources for Domestic Supply in Urban Areas (2018-2023)”. Here, we report on a) current research trends for studying urban drinking water systems during the last two decades and b) the development, testing, and integration of new methodologies, aiming for a better assessment, mapping, and management of water resources used for drinking water supply in urban settings. Selected examples of water isotope applications (Canada, USA, Costa Rica, Ecuador, Morocco, Botswana, Romania, Slovenia, India, and Nepal) provide context to the insights and recommendations reported and highlight the versatility of water isotopes to underpin seasonal and temporal variations across various environmental and climate scenarios. The study revealed that urban areas depend on a large spectrum of water recharge across mountain ranges, extensive local groundwater extraction, and water transfer from nearby or distant river basins. The latter is reflected in the spatial isotope snapshot variability. High-resolution monitoring (hourly and sub-hourly) isotope sampling revealed large diurnal variations in the wet tropics (Costa Rica) (up to 1.5‰ in δ 18O) and more uniform diurnal variations in urban centers fed by groundwater sources (0.08 ‰ in δ 18O) ([Ljubljana](https://www.google.com/search?client=firefox-b-1-d&sca_esv=f5a20a2e9138d638&sca_upv=1&sxsrf=ADLYWIKR6-DvBtjaWqFYRhn6VgnegOa8kg:1717189104058&q=Ljubljana&stick=H4sIAAAAAAAAAONgVuLQz9U3SMrNNXnEaMwt8PLHPWEprUlrTl5jVOHiCs7IL3fNK8ksqRQS42KDsnikuLjgmngWsXL6ZJUm5WQl5iUCAAFa64FOAAAA&sa=X&ved=2ahUKEwjMrrz047iGAxWyG9AFHSVwCBgQzIcDKAB6BAgTEAE), Slovenia). Similarly, while d-excess was fairly close to the global mean value (+10 ‰) across all urban centers (10-15‰), reservoir-based drinking water systems show significantly lower values (up to ~ -20 ‰) (Arlington, TX, USA and Gaborone, Botswana), as a result of strong evapoconcentration processes. δ 18O time series and depth-integrated sampling highlighted the influence of the catchment damping ratio in the ultimate intake water composition. By introducing new, traceable spatial and temporal tools that span from the water source to the end-user and are linked to the engineered and socio-economic structure of the water distribution system, governmental, regional, or community-based water operators and practitioners could enhance drinking water treatment strategies (including more accurate surface water blending estimations) and improve urban water management and conservation plans in the light of global warming.

The local temperarture cannot explain the inter-annual variation in δ18Oprecip in the coastal Antarctic in past few decades. To understand this enigmatic variation, we have used long-term modern δ18Oprecip value of three coastal Antarctic sites. Using the δ18O-d-excess relationship and modelled δ18O value of vapor at source, we have shown that δ18Oprecip inherits the signature of moisture source parameters (MSPs). Furthermore, the wavelet analysis suggests that the variation in the MSPs impacts the seasonal cycle of δ18Oprecip which lead to disparity in the seasonal isotope-temperature relationship. The Southern Ocean surface stratification, due to increase in the freshwater flux by glacier melting, led to alignment of MSPs in such a manner that altogether significantly lowered the isotopic composition of initially formed vapor, which is reflected in δ18Oprecip at inter-annual scale. Our observations suggest that the palaeothermometry will underestimate the Antarctic temperature change for the periods characterized by warming and high glacier-melt.