Purpose: To explore the feasibility of minimally invasive surgery（MIS）used in the treatment of neuroblastoma. Methods: Selected NB patients were randomly assigned to the MIS group and the open surgery（OS） group. The operative details, major complications, and prognosis of the two groups were compared. Results: A total of 35 children undergoing MIS and 35 children undergoing open surgery were enrolled in this study. According to the INRGSS staging system, 30 patients were classified as stage L1, 32 as stage L2, and 8 as stage M. No statistical difference was found in the age of the children and the maximum diameter of the primary site tumor between the two groups. The bleeding volume in the OS group was significantly higher than that in the MIS group (P=0.006), The time to start postoperative feeding in the MIS group was significantly shorter than that in the OS group (P<0.001). No significant difference was found in the number of GTR between the MIS group and the OS group (P=0.246). The one-year survival rate and overall survival rate of the MIS group were 100% and 93.85%, while the OS group was 100% and 93.72%, respectively. Conclusion: MIS has more advantages than OS for suitable neuroblastoma, while the prognosis is almost the same. After gradually improving the indications for MIS, it should become the preferred surgical method for children within this range.

Objective: To search for the DEGs between stage MS NB and stage M NB and speculate the possible mechanism of spontaneous regression of stage MS NB. Methods: The NB datasets GSE49710 and GSE45547 in the GEO database were selected to screen the DEGs between children with NB stage MS vs. stage M, < 18 months. GO enrichment and KEGG pathway analysis of DEGs was performed using DAVID. The intersecting genes among DEGs and RCD-related genes were selected, and their survival roles and functions were assessed. Results: BIRC5, SLCO4A1, POPDC3, and HK2 were found to be downregulated in stage MS NB and related to apoptosis. BIRC5 and HK2 also participate in autophagy. The TF gene is upregulated in stage MS NB and related to ferroptosis. The above five genes are closely related to the survival of children with NB. Conclusion: BIRC5, SLCO4A1, POPDC3, HK2 and TF are expected to become new important indicators to predict the prognosis of NB and can be used as the basis for further explored the benign prognosis and spontaneous regression mechanism of stage MS NB.