Brucellosis is the most common zoonotic infection over the world, caused by bacterial genus brucella. The disease is transmitted rarely via human-to-human transmission. Limited data support vertical transmission of human brucellosis. Herein, we reported a case of congenital brucellosis case. Brucella meliteusis DNA was detected by next-generation sequencing and bacterial identifying under microscope in mother’s placental specimen, which also proved chorioamnionitis. After the first course of rifampicin for 6 weeks and meropenem for 2 weeks, the disease relapsed within 3 weeks. The second course were rifampicin and SMZ/TMP for 8 weeks. The baby was still in following-up.