Global change alters both temperature regimes and spatial connectivity, reshaping the mechanisms that link local and regional biodiversity. We developed a temperature-dependent community model, parameterized with stream macroinvertebrate data, to assess how warming and dispersal influence population dynamics, community size, size structure, and temporal β-diversity in local communities. The model integrates temperature effects through metabolic body-size scaling and local recruitment inputs from a regional species pool to explore how energetic constraints cascade across scales. Warming reduced population and community sizes, increasing demographic stochasticity and leading to higher temporal β‐diversity. Enhanced connectivity produced the opposite pattern: larger, more stable communities with lower compositional turnover. Community size thus emerged as the critical mediator of biodiversity responses, integrating metabolic and spatial processes within a unified framework. Although warmer conditions lowered overall biomass and altered size‐spectra intercepts, slopes were more variable than predicted, reflecting stochastic fluctuations among small‐bodied species rather than systematic loss of large taxa. Together, these findings show that temperature‐driven metabolic acceleration and dispersal‐mediated rescue jointly determine biodiversity stability under global change. Our results highlight the role of community size in buffering or amplifying the effects of warming and provide a mechanistic bridge between metabolic theory and local-regional dynamics.

Historically, studies have examined how local habitat, resources and species interactions influence community structure in stream ecosystems. Increasingly, though, attention has turned to understanding how regional factors (e.g. dispersal) interact with local conditions to influence communities. Often dispersal of organisms occurs in spatially constrained habitats, which can drastically influence community assembly. Dendritic networks are an example, and have a branching spatial configuration with some branches of the system more connected to others, making dispersal easier, while other locations are more isolated. As interest in multi-scale community assembly mechanisms has increased, less work has focused on the relationship between community assembly and ecosystem processes. Here, we sought to understand how consumer-resource interactions unfold in river networks. We predicted that stream network location would mediate detritivore (shredder) richness and abundance, and in turn would be associated with a shift in decomposition of organic matter (leaf litter). To examine this, we manipulated leaf litter species in isolated (headwaters) and connected (mainstem) stream reaches. We found that shredder richness and abundance were influenced by both leaf litter quality and network location. Headwater environments supported a stronger consumer-resource relationship, and shredder communities were further richer and more abundant. This was not the case in mainstem locations. In these relatively harsher environments, we offer that shredders did not appear to be actively feeding on the resources, but rather utilizing leaf litter more for habitat. Our results suggest river network position has important implications for how ecosystem function changes across spatially constrained environments.

Despite a growing literature-base devoted to documenting biodiversity patterns in cities, little is known about the processes that influence these patterns, and whether they are consistent over time. In particular, numerous studies have identified the capacity of cities to host a rich diversity of plant species. This trend, however, is driven primarily by introduced species, which comprise a large proportion of the urban species pool relative to natives. Using an experimental common garden study, we assessed the relative influence of local assembly processes (i.e., soil environmental filtering and competition from spontaneous urban species) on the taxonomic and functional diversity of native plant communities sampled over four seasons in 2016-2018. Taxonomic and functional diversity exhibited different responses to local processes, supporting the general conclusion that species- and trait-based measures of biodiversity offer distinct insights into community assembly dynamics. Additionally, we found that neither soil nor competition from spontaneous urban species influenced taxonomic or functional composition of native species. Functional composition, however, did shift strongly over time and was driven by community-weighted mean differences in both measured traits (maximum height, Hmax; specific leaf area, SLA; leaf chlorophyll a fluorescence, chl a) and the relative proportions of different functional groups (legumes, annual and biennial-perennial species, C4 grasses, and forbs). In contrast, taxonomic composition only diverged between early and late seasons. Overall, our results indicate that native species are not only capable of establishing and persisting in vacant urban habitats, they can functionally respond to local filtering pressures over time. This suggests that regional dispersal limitation may be a primary factor limiting native species in urban environments. Thus, future regreening and management plans should focus on enhancing the dispersal potential of native plant species in urban environments, in order to achieve set goals for increasing native species diversity and associated ecosystem services in cities.