Analysis and Optimal Design of Batch and Two-Column Continuous
Chromatographic Frontal Processes for Monoclonal Antibody Purification
Abstract
Frontal chromatography has seen increased interest for protein
purification, in particular as a polishing step in downstream processes
for therapeutic proteins production, as for example the purification of
monoclonal antibodies (mAbs) from high molecular weight impurities,
e.g., aggregates, using cation exchange resins. In this work we
introduce a new two-column continuous process implementing frontal
chromatography. The design procedure and its performance, compared to
classical batch technology, are discussed. This represents an additional
option in the realisation of optimised continuous downstream processing
of therapeutic proteins.
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