For any wastewater treatment aeration tank, the paper presents a technique to deal with oxygen uptake rate (OUR) measurements that is primarily related to the biological uptake rate. he proposed dilution vs. the “shake it up” aeration approach to avoid shearing the floc which may increase the OUR artificially, jeopardizing the true measurement in the aeration tank. when the level of dissolved oxygen in the medium falls below a certain point, the specific rate of oxygen uptake is also dependent on the oxygen concentration in the liquid. Since it measures the rate at which oxygen is used, it is a useful tool to evaluate process performance, aeration equipment, and biodegradability of the waste. The OUR is a fundamental physiological characteristics of culture growth and has been used for optimizing the fermentation process, and so it needs to be measured accurately. Oxygen uptake rate (OUR) is the microorganism oxygen consumption per unit time and is one of the few accessible parameters to quantify the metabolism rate of the activated sludge in a wastewater treatment plant. To alleviate the many problems of measurement, the proposed method using dilution with saturated DO may give a more accurate measurement than the current standard method using a sample shaking technique as described in APHA 2017. With a more accurate measurement of the OUR, it may lend credence to justification for the modification of the fundamental equation for oxygen transfer in a respiring system, as applied to an example provided by ASCE/EWRI 18-18