Introduction: The aim was to analyze intracellular oxidised glutathione (GSSG)/reduced glutathione (GSH) and serum thiol/disulfide homeostasis tests in bacterial sepsis (BS) to evaluate the contribution of oxidative stress to etiopathogenesis. Methods: A total of 92 individuals, including 49 patients and 43 healthy volunteers, were included in the present study. The cases who had high probability of BS criteria defined by Singer et al. and positive blood culture were included in the study. The intracellular oxidised and reduced glutathione and thiol/disulfide homeostasis [total thiol-native thiol-disulfide changes] tests were used in the serum samples of 49 patients. Statistical analysis was performed by comparing the results with the control group. Results: When BS and control group were compared, GSH, total glutathione (GSH+GSSG), native thiol and total thiol levels were significantly lower in BS group compared to control group (p<.001). No significant differences were detected between the two groups in terms of GSSG levels. CRP, PCT, WBC and RDW levels were significantly higher in BS group than in control group (p<.001). Conclusions: Total thiol and native thiol levels in serum and intracellular glutathione levels decreased in BS. Disrupted intracellular GSH/GSSG and serum thiol/disulfide homeostasis might have effects on BS pathogenesis by increasing oxidative stress.

Objective: In the present study, the importance of albumin level in severe pneumonia due to covid 19 was investigated. Design: This was a retrospective study. Setting:Emergency Department of Ankara City Hospital, between 1 september 2020 and 1 february May 2021. Subjects: Effective triage and early detection are very important for the control and treatment of coronavirus disease 2019. For this purpose the relation between hypoalbuminemia and other acute phase reactants was compared in severe pneumonia due to Covid-19. Main outcome measures: The data of 122 patients diagnosed with pneumonia due to Covid 19 and 60 healthy control group were retrospectively analyzed in statistical terms in computer medium. The cases were divided into 3 groups as Healthy Control Group, Intubated Group, and Non-Intubated Group. The lung tomography of patients diagnosed with Covid 19 pneumonia was examined one-by-one. The RT-PCR (Real-Time Polymerized Chance Reaction) test results were recorded from the system. The albumin, WBC (White Blood Cell), N/L (Neutrophil/Lymphocyte Ratio), CRP (C-Reactive Protein) levels, who are acute phase reactant levels, of the patients were compared with the Control Group. Also, the two groups who were intubated and not intubated were also compared. Results: When all the data were examined, it was found that the albumin levels were lower at statistically significant levels in all 3 study groups (p<0.01). The other acute phase reactants, N/L ratio and CRP levels were significantly higher (p<0.05). Hypoalbuminemia was found to be significantly lower as a result of the comparisons of the two groups that were intubated and not intubated (p=0.02), and no differences were detected in terms of other parameters (p>0.05). Conclusion: Serum albumin levels may be lower in severe Covid 19 pneumonia. Hypoalbuminemia can be a biomarker indicating the severity of the disease as an acute phase reactant.

ABSTRACT Background:Metabolic changes seen after obstructive jaundice increase the load of oxidative stress.The thiol-disulphide homeostasis (TDB) has a vital importance as oxidatice stress biomarker. Aim: The aim of present study is to investigate the dynamic thiol-disulphide homeostasis in patients diagnosed with obstructive jaundice and to compare the dynamic thiol-disulphide homeostasis with ischemia modified albumin. Study design: A total of 146 participants who were admitted to emergency department and who consisted of 69 patients and 77 healthy volunteers, were included in the study. Detailed blood samples were obtained from the patients at the time of admission. Methods: Thiol / disulphide levels were examined using a novel method developed by Erel and Neselioglu on the patients diagnosed with obstructive jaundice during the admission. Data were evaluated in computer system. Results: The levels of total thiol (p =0.025) and native thiol (p =0.023) were statistically significantly lower in the patient with obstructive jaundice than in the control group. It was observed that there was a significant negative correlation between the native thiol levels and the ALP (r =-0.262, p<0.01), urea (r =-0.313, p<0.01), total bilirubin (r =-0.388, p<0.01), direct bilirubin (r =-0.351, p<0.01) levels. At the same time, the level of disulphide (p = 0.235) was lower in the patient group than in the control group but not statistically significant. It was found that the ischemia modified albumin (IMA) values were lower in the control group than the obstructive jaundice group (p = 0.03). Conclusion: Total thiol, native thiol and IMA levels decrease in obstructive jaundice pathogenesis.

Objective: There is a crucial balance between oxidant and antioxidant defense mechanisms. We aimed to evaluate the role of the balance of these systems in community-acquired pneumonia (CAP) in children. Methods: We analyzed serum oxidant and antioxidant stress parameters according to the clinical and demographic data of children with CAP and compared them with healthy controls. Serum total antioxidant status (TAS), total oxidant status (TOS), and levels of ischemia-modified albumin (IMA), antioxidant enzymes, non-enzymatic antioxidant factors, and plasma thiol were evaluated and compared between the groups. Results: Of 160 children evaluated, 106 had CAP (54 outpatients, 52 inpatients) and the other 54 were healthy subjects (control group). Total thiol and native thiol levels were significantly lower in the inpatient group compared to the outpatient group (p=0.004, p=0.005). Serum IMA differed significantly among the groups (p=0.001), with inpatients showing the highest level. A positive correlation was found between serum IMA and C-reactive protein levels in patients with pneumonia (r=0.351; p=0.001). Conclusion: Parameters that provide information about antioxidant capacity may be useful in the diagnosis and prognosis of pneumonia. Both thiol homeostasis parameters and IMA level seem likely to be influenced by disease severity. Our results suggest that plasma thiol levels and IMA may be good candidate biomarkers to predict the severity of pneumonia in children.