Human Immunodeficiency Virus (HIV), is a lethal virus that infects the human immune system. Once the person gets infected with HIV, the infection will gradually lead to AIDS which attacks the immune system and ultimately causes death if proper precautionary measures are not taken against it. Currently, anti-viral therapies and drugs combined with this are utilized by patients diagnosed with HIV. But with rapid mutation capabilities, the virus showcased resistance to the drugs, therefore, pushing the scientific community to explore a more stable and rapid treatment option for HIV. Recently, the utilization of CRISPR Cas technologies for genetic modification, disease diagnosis, disease treatment, and biosensing technologies has greatly increased due to their numerous advantages. This review focuses on the working mechanism of CRISPR Cas13a that is divided into its stages followed by the conventional methods used for HIV treatment, we also highlighted the potential applications that use Cas13 protein for its working principle and finally the usage of Cas13a towards HIV diagnosis, treatment, and prospects of this technology.