Bothrops jararacussu (BjsuV) snake bites cause severe local and systemic effects leading to muscle damage and amputation that cannot be prevented by antivenom alone. The anti-inflammatory effects of Photobiomodulation (PBM) has been noted to reduce muscle damage and promote tissue regeneration. This study investigated the mechanism of PBM in modulating macrophage functions via TGF-β1 singlaing. Macrophages were evaluated after treatments with BjsuV, TGF-β1, TGF-β inhibitor - SB431542, and PBM with 660 nm red or 810 nm near-infrared laser at 10 mW/cm 2, 400 and 500s, 4 J/cm 2 and 5 J/cm 2, 7.5 p.J/cm 2, 1.7ɇ). BjsuV significantly reduced macrophage survival and migration, but increased phagocytosis. Treatment with PBM significantly counteracted these venom-induced inflammatory responses. Recombinant TGF-β1 treatments stimulated migration and reduced phagocytosis while preincubation with SB431542 only partially neutralized PBM responses. These observations indicate the anti-inflammatory effects of PBM in macrophages contribute to its therapeutic potential in mitigating venom-induced multiple signaling pathways.