Interactions between topography and the spatial distribution of cypress give rise to the spatial heterogeneity of surface runoff on steep forested slopes in southwest China. To reduce surface runoff and improve the water conservation capacity of cypress forests, the coupling effects of topography and the spatial distribution of cypress on surface runoff coefficient were studied through the Structural Equation Modeling (SEM) and the Response Surface Method (RSM) based on twelve natural runoff plots. Results showed that the surface runoff coefficient increased monotonically with the increase of the composite index of topography (topographic relief× runoff path density/ surface roughness), and increased first and decreased later with the increase of the composite index of the spatial distribution of cypress (stand density of cypress× contagion index of cypress). To reduce surface runoff coefficient from a larger value (>0.5) to less than 0.3, two strategies of stand structure adjustment could be adopted, including only increasing the stand density of cypress or increasing both the stand density and the contagion index of cypress, and which strategy should be adopted depended on the initial stand density of cypress. When the initial stand density of cypress was relatively low (<20 ind/100m2), the first step was to increase the stand density of cypress, and until the stand density of cypress reached to a moderate level (20-50 ind/100m2), adjusting the spatial structure of cypress from relatively regular to relatively clumped could reduce surface runoff coefficient to a greater extent.

Reinfection after getting covid19 once was thought not to happen as patient would have developed immunity against the virus. But recently a case has been reported from Hongkong where the second episode of asymptomatic infection occurred 142 days after the first symptomatic episode in an apparently immunocompetent patient. Epidemiological, clinical, serological and genomic analyses confirmed that the patient had re-infection with another strain of SARSCOV2 virus instead of persistent viral shedding from first infection. Here we report two children who got re-infected with SARSCOV2 after recovering fully and developing IgG antibodies it.

Background: Endocrine disturbances are frequent long-term complications of acute lymphoblastic leukemia (ALL) treatment. Research on the risk of impaired linear growth and overweight has reported conflicting results. Procedure: A longitudinal, retrospective study for the characterization of growth (height and body mass index (BMI)) was performed, based on the clinical records of patients treated for ALL since 2003 and off treatment for a minimum of two years. Data on height and weight were collected at diagnosis (0M) and at 6, 12, 24 and 48 months (M), as well as the most recent height (FH). Effects of cranial radiotherapy (CRT) and sex on growth changes were evaluated. FH was compared with target height (TH). Results: 78 patients (52.5% males) met the inclusion criteria. CRT was used in 28.2%. Height percentile (HP) was reduced at 6M reaching a minimum at 12M; this recovered at 48M, but was still inferior to diagnosis for females and most significantly in the CRT group. Diagnosis HP was in general higher than TH. Overweight/obesity affected 21.8% patients at 0M, 45.9% at 12M and 71.4% at 24M. BMI percentile (BP) decreased from 24M to 48M but was still higher than at 0M. The CRT-group had no significant decrease in BP from 24M to 48M. Conclusions: ALL treatment affected linear growth and caused an increase in BMI, with a higher impact on CRT-treated patients for both studied parameters and in females only for height. FH appeared not to be inferior to patient’s genetic potential. BMI remained increased after treatment.

Bone marrow necrosis (BMN) is a rare pathologic finding, but when encountered is most often associated with malignancy. In adults its presence correlates with an inferior prognosis, however in children the prognostic implication is unclear. We performed a retrospective review of 3,760 bone marrow specimens in patients ≤ 18 years over a ten-year period. BMN was identified in less than 1% of specimens and only in patients with leukemia, lymphoma or neuroblastoma. BMN contributed to a delay in diagnosis; however, advanced medical imaging may serve as a tool to localize non-necrotic areas for bone marrow sampling, facilitating an expedited diagnosis.

Deep learning has emerged as a revolutionary technology for protein residue-residue contact prediction since the 2012 CASP10 competition. Considerable advancements in the predictive power of the deep learning-based contact predictions have been achieved since then. However, little effort has been put into interpreting the black-box deep learning methods. Algorithms that can interpret the relationship between predicted contact maps and the internal mechanism of the deep learning architectures are needed to explore the essential components of contact inference and improve their explainability. In this study, we present an attention-based convolutional neural network for protein contact prediction, which consists of two attention mechanism-based modules: sequence attention and regional attention. Our benchmark results on the CASP13 free-modeling (FM) targets demonstrate that the two attention modules added on top of existing typical deep learning models exhibit a complementary effect that contributes to predictive improvements. More importantly, the inclusion of the attention mechanism provides interpretable patterns that contain useful insights into the key fold-determining residues in proteins. We expect the attention-based model can provide a reliable and practically interpretable technique that helps break the current bottlenecks in explaining deep neural networks for contact prediction. The source code of our method is available at https://github.com/jianlin-cheng/InterpretContactMap.

Our aim in this paper is to give a sufficient condition ensuring the finite-time attractivity for the zero solution to semilinear functional differential inclusions in Banach spaces, in the case where the nonlinearity function possibly has superlinear growth. Our analysis is based on the semigroup theory, the fixed point principle for condensing multi-valued maps, and local estimates of solutions. The abstract results will be applied to a class of polytope inclusions in $C_0$ setting.

This paper considers minimizers of the following inhomogeneous $L^2$-subcritical energy functional \[E(u):=\int_{\R^N}|\nabla u|^{2}dx-\frac{2}{p+1}\int_{\R^N}m(x)|u|^{p+1}dx,%\ u\in H^{1}(\R^N), \] under the mass constraint $\|u\|^{2}_{2}=M$. Here $N\geq1$, $p\in(1,1+\frac{4}{N})$, $M>0$ and the inhomogeneous term $m(x)$ satisfies $0

This article proposes a mathematical model describing the evolution and transmission of Varicella Zoster Virus (VZV) over large groups of individuals. The model was formulated to accommodate parameters and variables describing direct and indirect forms of transmission, re-activation of infectious shingles as well as treatment and vaccination of susceptible births and influx of immigrants. The model was analysed to be positive, bounded and well posed. The controlled basic reproduction number Rvzv, obtained using the next generation matrix operator reveal that vaccination is effective as a control in creating a level herd immunity. Linearizing the model around the VZV - free equilibrium shows that the model is locally and globally asymptotically stable when Rvzv is less than unity. The approximate solutions of the model system equations was obtained using the modified differential transform which involves the Differential Transform Method (DTM) and Laplace - Pade post-treatment technique (LPDTM). This technique was employed to enlarge the domain of convergence of the approximate solutions of the model. LPDTM was compared with the Fehlberg fourth order Runge - Kutta (RKF45) via the MAPLE computational software to show the accuracy of the results through simulations. Further simulations carried out on the model reveal that timely vaccination and treatment are eeffective strategies in containing VZV infection spread in human and environmental host population.

Background and purpose Previous studies have reported that Portulaca oleracea L. polysaccharides (POL-P3b) is an immunoregulatory agent. However, POL-P3b as a novel immune adjuvant in combination with DC vaccine for breast cancer treatment has been little studied. In this work, DC vaccine loaded with mouse 4T1 tumor cell antigen was prepared to evaluate the properties of POL-P3b in inducing maturation and function of DC derived from mouse bone marrow, and then to investigate the effect of DC vaccine combined with POL-P3b on breast cancer in vivo and in vitro. Experimental approach Morphological changes of DC were observed using scanning electron microscopy. Phenotypic and functional analysis of DC were detected by flow cytometry and allogeneic lymphocyte reaction. Cytokine levels in DC culture supernatant were detected by ELISA. Western blotting analysis for the protein expression of TLR4, MyD88 and NF-κB. Apoptosis detection and protein expression of tumor tissue were analysed by TUNEL staining and immunohistochemistry, respectively. To evaluate the security of POL-P3b by detecting hematological, blood biochemical indicators and pathological analysis for liver and kidney Key results POL-P3b can induce DC activition and maturation, attributing to increasing specific anti-tumor immune response, and the mechanism of action involved in the TLR4/MyD88/NF-κB signaling pathway. Experimental results in vivo further suggested that administration of POL-P3b-treated antigen-primed DC achieved remarkable tumor growth inhibition through inducing apoptosis. Moreover, POL-P3b-treated DC vaccine was able to inhibit lung metastases. Conclusions and implications The results proved the feasibility of POL-P3b as an adjuvant of DC vaccine for anti-breast cancer therapy.

Objective: The aim of this study is to define the correlation between intramyocardial left anterior descending artery (IMLAD) and coronary artery angiography (CAG); our clinical intraoperative experiences. Material and Methods: Between January 2014 and May 2019, 196 patients underwent coronary artery bypass grafting (CABG) surgery in Bozok University Medicine Faculty Hospital. The correlation between the typical “wide-U” image of left anterior descending artery (LAD) depression(Cicekcioglu sign) on CAG and our intraoperative surgical observations of the patients were studied. Results: Of the 196 patients, 5 were excluded due to the total occlusion of proximal LAD. 30 had a typical “wide-U” image(Cicekcioglu sign) on CAG and 22 were observed to have an IMLAD. Of the 25 patients being observed to have IMLAD intraoperatively, 3 had a normal preoperative CAG imaging. The prevalence of the IMLAD was 13%. Sensitivity of CAG was measured as 88% and specificity of CAG was 95.1%. Discussion: In CAG, in case of LAD composing a “wide-U” imaging(Cicekcioglu sign) the image of LAD course is often observed to have a correlation with the intraoperative detection of IMLAD.

The use of genomic and phenotypic data to scan for outliers is a mainstay for studies of hybridization and speciation. Geographic cline analysis of natural hybrid zones is widely used to identify putative signatures of selection by detecting deviations from baseline patterns of introgression. As with other outlier-based approaches, demographic histories can make neutral regions appear to be under selection and vice versa. In this study, we use a forward-time individual-based simulation approach to evaluate the robustness of geographic cline analysis under different evolutionary scenarios. We measured the effects of drift on genetic differentiation, and on false positive and false negatives detection using geographic clines. We modeled multiple stepping stone hybrid zones with distinct age, deme sizes, and migration rates, and evolving under different types of selection. We found that in young hybrid zones, drift increases overall genomic divergence, distorts cline shapes and increases both false positive and false negative rates. In old hybrid zones, genomic divergence and cline distortion are higher. Our results suggest that geographic clines are most useful for outlier analysis in young hybrid zones with large populations of hybrid individuals.

Early and long term Clinical outcome after Minimally Invasive Direct Coronary Bypass Grafting versus off pump Coronary Surgery via Sternotomy In this retrospective study by Cichon Romuald et al (1), 194-patients met the inclusion criteria and were divided into the MIDCAB group (n=111) and OPCAB via median sternotomy group (n=83). The conclusion was that short as well as long-term outcomes of MIDCAB in terms of mortality, myocardial infarction, stroke, and target vessel revascularization were satisfactory, and as safe and effective as OPCAB via sternotomy This retrospective study by Cichon Romuald et al (1), undoubtedly will generate interest for surgeons who want to perform minimally invasive coronary surgery. It is clear that, in order to attract patients to undergo surgery, surgeons must learn to perform minimally invasive coronary surgery. Advantages of minimally invasive coronary surgery include less post-operative discomfort, faster healing times, less risk of infection and avoidance of trauma associated with OPCAB Surgeons must also take the initiative and play an active role in the Hybrid Revascularization Procedures Current surgeons, and those in residency training programs, should learn wire skills and participate in placement of stents. There is a lot to learn from our interventional cardiologists who embrace new technology and procedures. Surgeons will have to adapt to the new reality, and move some of his/her practice outside the operating room.

Climate and land-use change are some of the most profound threats to the biodiversity and functioning of the Earth’s ecosystems, yet potential synergistic effects remain unclear. Here we examined how aridity and land-use (overgrazing and haying) affect aboveground biomass and soil organic carbon (SOC) through changes in plant species richness across 716 grassland sites in northern China. We found that aridity and grazing reduced aboveground biomass and SOC through decreasing plant species richness. Notably, we observed strong negative synergistic effects of aridity and grazing, suggesting that soil carbon storage was particularly threatened by grazing in arid environments. By contrast, haying reduced aboveground biomass and had no significant effect on SOC, although it increased plant species richness. Together, the results demonstrate that climate change and overgrazing mainly threaten soil carbon storage via their detrimental effects on plant diversity, and the detrimental overgrazing effects are particularly strong under arid conditions.

Background. Pulmonary veins (PV) reconnection is the most common reason for atrial fibrillation (AF) recurrence. The ablation-index is a marker of ablation lesion quality which use achieves high percentages of first pass isolation and improved results of AF ablation. Most operators use a double trans-septal approach with confirmation of PV isolation with a circular mapping catheter. In the present study we aimed to show that an ablation-index guided procedure using a single trans-septal approach and ablation catheter only would achieve adequate PV isolation while demonstrating the critical role of the carina in PV isolation. Methods. 76 consecutive patients with paroxysmal AF: 34 patients underwent WACA, 32 patients underwent WACA+ (including empiric carina isolation) and 10 patients underwent a staged procedure of WACA followed by WACA+ in case of lack of first pass isolation. All procedures were performed via single trans-septal. Results. Compared to WACA-only, WACA+ increased the odds of PV isolation from 65% to 91%, p=0.012. In WACA-only, ablation of the carina was needed to achieve PV isolation. The role of the carina was confirmed in 10 patients with sequential ablation. PV isolation was confirmed by inserting a circular mapping catheter through the single trans-septal sheath. At 18 months of follow-up [IQR 15.2-20.8 months], freedom from AF was 84% for the entire cohort. Conclusion. Our study confirms the high success rate of PV isolation using ablation index and shows that this can be achieved via single trans-septal crossing. Our study confirms the role of the carina in PV isolation.

NADPH:protochlorophyllide (Pchlide) oxidoreductase (POR) is a key enzyme of chlorophyll biosynthesis in angiosperms. It is one of few known photoenzymes, which catalyzes the light-activated trans-reduction of the C17-C18 double bond of Pchlide’s porphyrin ring. Due to the light requirement, dark-grown angiosperms cannot synthesize chlorophyll. No crystal structure of POR is available, so to improve understanding of the protein’s three-dimensional structure, its dimerization, and binding of ligands (both the cofactor NADPH and substrate Pchlide), we computationally investigated the sequence and structural relationships among homologous proteins identified through database searches. The results indicate that α4 and α7 helices of monomers form the interface of POR dimers. On the basis of conserved residues, we predicted 11 functionally important amino acids that play important roles in POR binding to NADPH. Structural comparison of available crystal structures revealed that they participate in formation of binding pockets that accommodate the Pchlide ligand, and that five atoms of the closed tetrapyrrole are involved in non-bonding interactions. However, we detected no clear pattern in the physico-chemical characteristics of the amino acids they interact with. Thus, we hypothesize that interactions of these atoms in the Pchlide porphyrin ring are important to hold the ligand within the POR binding site. Analysis of Pchlide binding in POR by molecular docking and PELE simulations revealed that the orientation of the nicotinamide group is important for Pchlide binding. These findings highlight the complexity of interactions of porphyrin-containing ligands with proteins, and we suggest that fit-inducing processes play important roles in POR-Pchlide interactions.

Tegoprazan, a novel potassium-competitive acid blocker, is used to treat acid-related diseases. However, there is no information on the pharmacokinetic (PK) and pharmacodynamic (PD) profiles of the marketed dosage of tegoprazan under various meal timings in a fed and fasted state. The study aimed to assess the effect of meal timing on PKs and PDs of tegoprazan 50 mg after a single administration in healthy male subjects. An open-label, single-dose, three-treatment, three-period crossover study was conducted. A total of 12 subjects were orally administered a single dose of tegoprazan 50 mg among various conditions: in a fasted state, at 30 min before or 30 min after a high-fat meal. PK parameters were estimated by non-compartmental method. Continuous 24-hour intragastric pH monitoring was done for PD analysis. The PKs and PDs of tegoprazan were compared among the various meal timings. Compared to the fasting condition, the PK profile of tegoprazan was similar when administered 30 min before a high-fat meal; however, delayed absorption with similar systemic exposure was observed when administered 30 min after a high-fat meal. The magnitude of acid suppression evaluated through the PD parameters increased when administered 30 min after a high-fat meal compared to fasting the condition and when administered 30 min before a high-fat meal. However, the increased difference in acid suppression was not clinically significant. Meal timing had no clinically significant effect on the PKs and PDs of tegoprazan 50 mg. Therefore, the marketed dosage of tegoprazan could be administered regardless of the meal timing.

Introduction The ORBITA trial of PCI versus a placebo procedure for patients with stable angina was conducted across 6 sites in the United Kingdom via home monitoring and telephone consultations. Patients underwent detailed assessment of medication adherence which allowed us to measure the efficacy of the implementation of the optimisation protocol and interpretation of the main trial endpoints. Methods Prescribing data were collected throughout the trial. Self-reported adherence was assessed, and urine samples collected at pre-randomisation and at follow-up for direct assessment of adherence using HPLC MS/MS. Results Self-reported adherence was >96% for all drugs in both treatment groups at both stages. The percentage of samples in which drug was detected at pre-randomisation and at follow-up in the PCI vs. OMT groups respectively was: clopidogrel, 96% vs. 90% and 98% vs. 94%; atorvastatin, 95% vs. 92% and 92% vs. 91%; perindopril, 95% vs. 97% and 85% vs. 100%; bisoprolol, 98% vs. 99% and 96% vs. 97%; amlodipine, 99% vs. 99% and 94% vs. 96%; nicorandil, 98% vs. 96% and 94% vs. 92%; ivabradine, 100% vs. 100% and 100% vs. 100%; and ranolazine, 100% vs. 100% and 100% vs. 100%. Conclusions Adherence levels were high throughout the study when quantified by self-reporting methods and similarly high proportions of drug were detected by urinary assay. The results indicate successful implementation of the optimisation protocol delivered by telephone, an approach that could serve as a model for treatment of chronic conditions, particularly as consultations are increasingly conducted online.

Aims PC945 is a novel antifungal triazole for nebulised delivery to treat lung Aspergillus infections. Pharmacokinetic and safety profiles from nonclinical studies and clinical trials in healthy subjects and subjects with mild asthma were characterised. Methods Toxicokinetics were assessed following daily 2-hour inhalation for 14 days. Drug-drug interactions were evaluated using pooled human liver microsomes. Clinical safety and pharmacokinetics were assessed following (i) single inhaled doses (0.5–10 mg), (ii) 7-day repeat doses (5 mg daily) in healthy subjects; (iii) a single dose (5 mg) in subjects with mild asthma. Results Cmax occurred 4 hours (rats) or immediately (dogs) after a single dose. PC945 lung concentrations were substantially higher (>2000-fold) than those in plasma. PC945 only inhibited CYP3A4/5 substrate metabolism (IC50: 1.33 µM [testosterone] and 0.085 µM [midazolam]). Geometric mean Cmax was 322 pg/mL (healthy subjects) and 335 pg/mL (subjects with mild asthma) 4–5 hours (median tmax) after a single inhalation (5 mg). Following repeat, once daily inhalation (5 mg), Day 7 Cmax was 951 pg/mL (0.0016 µM) 45 minutes after dosing. Increases in Cmax and AUC0–24h were approximately dose proportional (0.5-10 mg). PC945 administration was well tolerated in both healthy subjects and subjects with mild asthma. Treatment-emergent adverse events were mild/moderate and resolved before the study ended. No clinically significant lung function changes were observed. Conclusions PC945 pharmacokinetics translated from nonclinical species to humans showed slow absorption from lungs and low systemic exposure, thereby limiting the potential for adverse side effects and drug interactions commonly seen with systemically delivered azoles.

Understanding trade-offs in wild populations is difficult, but important if we are to understand the evolution of life histories and the impact of ecological variables upon them. Markers that reflect physiological state and predict future survival would be of considerable benefit to unravelling such trade-offs and could provide insight into individual variation in senescence. However, currently used markers often yield inconsistent results. One underutilised measure is haematocrit, the proportional of blood comprising of erythrocytes, which relates to the blood’s oxygen-carrying capacity and viscosity, and to individual endurance. Haematocrit has been shown to decline with age in cross-sectional studies (which may be confounded by selective appearance/disappearance). However, few studies have tested whether haematocrit declines within-individuals or whether low haematocrit impacts survival in wild taxa. Using longitudinal data from the Seychelles warbler (Acrocephalus sechellensis), we demonstrated that haematocrit increases with age in young individuals (<1.5 years) but decreases with age in older individuals (1.5–13 years). In breeders, haematocrit was higher in males than females and varied relative to breeding stage. High haematocrit was associated with lower survival in young individuals, but not older individuals. Thus, while we did not find support for haematocrit as a marker of senescence, high haematocrit is indicative of poor condition in younger individuals. Possible explanations are that these individuals were experiencing dehydration and/or high endurance demands prior to capture, which warrants further investigation. Our study demonstrates that haematocrit can be an informative metric for life-history studies investigating trade-offs between survival, longevity and reproduction.

Using an immersed boundary-lattice Boltzmann method, we investigated the response of dense granular suspensions to time-varying shear rates and flow reversals. The apparent viscosity and the evolution of particle clusters were analysed. The solids fractions and particle Reynolds numbers varied over the ranges 5% ≤ φv ≤ 47% and 0.11 ≤ Rep ≤ 0.32. The simulations included sub-grid scale corrections for unresolved lubrication forces. The contribution of the tangential lubrication corrections to the shear stress is dominant when φv surpasses 30%. For φv > 35%, increasing shear-thickening is seen with increasing φv. Following a shear reversal, the number of clusters temporarily increases and then decreases to a stable value over the same time scale as the development of the wall shear stress (and apparent viscosity). Simulations with several step changes in the shear rate show the effects of the previous shear history on the viscosity of the suspension.

Aging effects of off-gas streams including dry air and humid air on reduced silver exchanged mordenite (Ag0Z) were studied. Aged Ag0Z was prepared by exposing Ag0Z to dry air and humid air at different aging temperatures, time, and water vapor concentrations. Iodine loading capacity on the aged Ag0Z was obtained through a continuous-flow adsorption system. Significant iodine loading capacity losses were observed after the Ag0Z was exposed to dry air and humid air. Physical and chemical analyses were conducted to observe the physical and chemical changes of Ag0Z after being aged. From iodine adsorption data and sample analyses, it was found that iodine loading capacity on the aged Ag0Z in dry air and humid air decreases with increasing aging temperatures, time and water vapor concentrations. The pseudo reaction model describes experimental data well and the oxidation of Ag0 is the rate determining step in the aging process.

Cysteinyl leukotrienes (CysLTs) are inflammatory lipid mediators that play a central role in the pathophysiology of several inflammatory diseases. Recently, there has been an increased interest in determining how these lipid mediators orchestrate tumor development and metastasis through promoting a pro-tumoral microenvironment. Upregulation of CysLTs receptors and CysLTs production is found in a number of cancers and has been associated with increased tumorigenesis. Understanding the molecular mechanisms underlying the role of CysLTs and their receptors in cancer progression will help investigate the potential of targeting CysLTs signaling for anti-cancer therapy. This review gives an overview of the biological effects of CysLTs and their receptors, along with current knowledge of their regulation and expression. It also provides a recent update on the molecular mechanisms that have been postulated to explain their role in tumorigenesis and on the potential of anti-CysLTs in the treatment of cancer.

Peste des Petits Ruminants (PPR) is a highly contagious viral disease that mainly affects goats and sheep in Asia, Africa and the Middle East. The PPR virus (PPRV) can be classified into four genetically distinct lineages (I, II, III, and IV). All have been historically present in Africa, except the Asian lineage IV that has been spreading across the globe and Africa for the last decades. Previous studies have identified the presence of the lineage IV in Nigeria since 2010. In this study, samples were taken from 268 small ruminants with PPR symptoms across Nigeria in 2017-2018 to provide an update on the distribution and genetic diversity of the lineage IV in the country. Sequence from a portion of the PPRV nucleoprotein (N) gene could be obtained from 72 samples, and all but one belonged to lineage IV. Phylogenetic analysis identified at least 5 lineage IV sub-clusters in Nigeria, re-grouping samples across multiple regions. Our results suggest an extensive endemic circulation of PPRV lineage IV across Nigeria and across borders with neighbouring countries, highlighting the difficulty of the control of this disease in the region.

Objective This study was performed to determine the effect of “工”-shape partial colpocleisis (modified LeFort partial colpocleisis) in elderly women with advanced pelvic organ prolapse. Design A retrospective cohort study. Setting One public hospital in China. Population 67 women with grade 3 or 4 POP undergoing surgery from January 2015 to December 2019. Methods This retrospective cohort study involved women who underwent “工”-shape partial colpocleisis for advanced pelvic organ prolapse. We collected data regarding the patients’ baseline characteristics, comorbidities, severity of prolapse, operation time, and postoperative complications. Subjective postoperative outcomes and patient satisfaction levels were also assessed. Main Outcome Measures Operation time, intraoperative blood loss, postoperative hospital stay, operative complications and patients’ satisfaction. Results Overall, 67 women were included. The mean operation time was 71.3 ± 21.7 minutes, and the mean intraoperative blood loss was 81.3 ± 36.7 ml. The mean postoperative hospital stay was 4.6 ± 3.0 days. No intraoperative complications occurred, and 8.96% of patients had postoperative complications. All patients were satisfied with the outcome of the “工”-shape partial colpocleisis procedure. Conclusion The “工”-shape partial colpocleisis procedure had a high satisfaction rate and a positive impact on pelvic symptoms. It was associated with minimal adverse events in women with advanced apical pelvic organ prolapse. This procedure should be considered a treatment option for advanced apical pelvic organ prolapse in selected elderly women who do not wish to conserve their vaginal coital function.