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TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the...
Amy Schmid
Rylee K. Hackley

Amy Schmid

and 9 more

June 11, 2023
Microbial cells must continually adapt their physiology in the face of changing environmental conditions. Archaea living in extreme conditions, such as saturated salinity, represent important examples of such resilience. The model salt-loving organism Haloferax volcanii exhibits remarkable plasticity in its morphology, biofilm formation, and motility in response to variations in nutrients and cell density. However, the mechanisms regulating these lifestyle transitions remain unclear. In prior research, we showed that the transcriptional regulator, TrmB, maintains the rod shape in the related species Halobacterium salinarum by activating the expression of enzyme-coding genes in the gluconeogenesis metabolic pathway. In Hbt. salinarum, TrmB-dependent production of glucose moieties is required for cell surface glycoprotein biogenesis. Here we use a combination of genetics, quantitative phenotyping, and gene expression assays to demonstrate that TrmB is essential for growth under gluconeogenic conditions in Hfx. volcanii. The ∆ trmB strain rapidly accumulated suppressor mutations in a gene encoding a novel transcriptional regulator, which we name Trm B su ppressor, or TbsP. TbsP is required for adhesion to abiotic surfaces (i.e. biofilm formation), and maintains wild type cell morphology, and motility. TrmB and TbsP jointly regulate the glucose-dependent transcription of gapII, which encodes an important gluconeogenic enzyme. We conclude that TrmB and TbsP co-regulate gluconeogenesis, with downstream impacts on lifestyle transitions in response to nutrients in Hfx. volcanii.
Evaluation of Left Ventricular Function in Patients with Mitral Annular Disjunction U...
Omer Dogan
Serhan Ozyildirim

Omer Dogan

and 9 more

June 11, 2023
Introduction: Mitral annular disjunction (MAD) is a structural abnormality characterized by the systolic detachment of the posterior mitral annulus and the ventricular myocardium. It is usually observed coexistent with mitral valve prolapse(MVP) and associated with a mechanical dysfunction despite preserved electrical isolation function of the mitral annulus. Diagnosis depends on the detection of disjunction by imaging modalities as transthoracic echocardiography or cardiac magnetic resonance imaging (CMRI). This study aimed to evaluate left ventricular (LV) function using speckle tracking echocardiography in MVP patients with MAD. Methods: This study was designed as a prospective, single-center study including 103 patients with MVP and 40 age- and sex-matched control subjects. Transthoracic echocardiography and cardiac magnetic resonance imaging were performed to assess LV function and MAD presence. Results: MAD (+) MVP (n=34), MAD (-) MVP (n=69), and control (n=40) groups were enrolled in the study. Among the MVP patients, 34 (33%) had MAD. T negativity in the inferior leads on electrocardiography was more frequent in the MAD (+) group than in the MAD (-) patients (4.3% vs 20.6%, p=0.014). Mitral regurgitation degree, Pickelhaube sign (17.6% vs. 1.4%, p=0.005), and late gadolinium enhancement frequency (35.3% vs. 10.6%, p=0.002) were significantly higher in MAD (+) patients. MAD (+) patients had significantly impaired global longitudinal strain (GLS) -23.1±2.1 vs. -23.5±2.3, P<0.001), basal longitudinal strain (BLS) (-19.6±1.5 vs -20.5±1.9, P < 0.001), and Mid-Ventricular Longitudinal Strain(MVLS) (-22.2±1.7 vs -23.2±2.2, P<0.001) when compared to MAD (-) MVP patients, despite similar LV ejection fraction. All these values of MVP patients were also significantly lower than the control group. The mean MAD distance was 7.8 ± 3.2 mm in MAD (+) patients. Patients with 2 or more symptoms were higher in the MAD (+) group than in the MAD (-) group (4.3% vs 44.1%, P<0.001). Conclusion: This study demonstrated a significant decrease in longitudinal strain in MVP patients with MAD, indicating myocardial dysfunction. These findings suggest that MAD may contribute to LV dysfunction and highlight the importance of early detection in younger patients. Further research is needed to explore the functional implications and long-term outcomes of MAD.
Availability of Different Branded Generic Vildagliptin after Off-patenting: An Observ...
Shambo Samajdar
Shatavisa Mukherjee

Shambo Samajdar

and 5 more

June 10, 2023
This cross-sectional observational study evaluated the availability and switching patterns of branded generics of Vildagliptin, an antidiabetic medication, in a real-world setting. Data from 967 prescriptions obtained from various pharmacies over a 3-month period post-patent expiration were analyzed. The study revealed a diverse range of branded generics, highlighting challenges in consistent availability. Switching patterns were observed, with a significant proportion of patients deviating from the initially prescribed branded generic. These findings emphasize the need for strategies to improve availability and promote appropriate use of branded generics, enhancing cost-effective diabetes management and patient care. Further research is warranted to investigate factors influencing brand switching behaviors and their impact on patient outcomes. This study provides valuable insights for optimizing the prescribing and availability of branded generics, ultimately enhancing the quality of care for patients requiring Vildagliptin treatment.
Research on typical fault detection of AC motor stator based on open transformer meth...
yucai wu
long zhang

yucai wu

and 1 more

June 12, 2023
The general diagnosis of typical faults of AC motor stator can simplify the detection steps, improve the detection efficiency, and help to shorten the outage time of motor maintenance. In this paper, the open transformer method is proposed to detect the inter-turn short-circuit (ITSC) fault of stator winding and the inter-lamination short circuit fault of stator core. Firstly, the expression of the flux in the open transformer core before and after the typical fault of the AC motor stator is derived. The expression of the induced electromotive force of the open transformer winding is further obtained, and the variation law of the induced electromotive force before and after the fault is obtained. Different finite element electromagnetic simulation models are established for the two typical faults, and experimental schemes are designed for verification. The final simulations and experiments show that the amplitude of the induced electromotive force of the open transformer winding changes significantly after the typical fault of the AC motor stator , and its amplitude varies with the fault location and fault degree, which proves the feasibility of the open transformer method for the general detection of the typical fault of the AC motor stator.
Vulnerable Node Identification Method for Distribution Networks Based on Complex Netw...
Enyu Jiang
Wentao Zhang

Enyu Jiang

and 5 more

June 12, 2023
A method for identifying vulnerable nodes in distribution networks is proposed, which is based on complex networks and optimized TOPSIS. This method aims to address the issues of one-sided evaluation indicators and inaccurate indicator weights that are present in existing methods for identifying vulnerable nodes in distribution networks. Based on the theory of complex networks, a comprehensive set of vulnerability indicators for distribution network nodes is constructed by considering both the topology structure and system operation status of the distribution network. The TOPSIS comprehensive evaluation model for optimization is proposed to enhance the selection process of optimal and worst indicator values. The advantages and disadvantages of each indicator are characterized using Mahalanobis distance. The calculation of proximity is optimized by establishing a virtual negative ideal solution, which makes the identification of vulnerable nodes more reasonable. The simulation results demonstrate that this method is more effective in identifying vulnerable nodes in the power grid compared to traditional methods, and has significant practical applications.
Reactive Power Output Modeling of Synchronous Condenser in UHVDC Converter Station Ba...
lin wang
Honghua Wang

lin wang

and 3 more

June 12, 2023
For sake of guaranteeing the stable operating of power system, synchronous condenser (SC) is configured in ultra-high voltage direct current (UHVDC) converter station for affording dynamic reactive power support to power system. Focusing on reactive power regulation system of SC with strong coupling, multivariable, and nonlinear, it is difficult for the universal analytic method to exactly build reactive power output model of SC. Reactive power output model of SC in UHVDC converter station based on interlaced superposition convolutional neural network-bidirectional long short-term memory (CNN-BiLSTM) is presented. The excitation voltage, excitation current of SC are used as inputs for training and test sampled data, and the reactive power of SC is used as outputs for training and test sampled data. CNN model based on interlaced superposition is superimposed of two convolution units with different structures to extract the feature of sample data, which are transformed into feature vectors and input into BiLSTM model. Bayesian optimization method is used to optimize its hyper parameters. The predicted results indicate that the proposed reactive power output model of SC in UHVDC converter station based on interlaced superposition CNN-BiLSTM improves modeling accuracy and generalization ability, and provides a reference for SC reactive power regulation control.
Security-Constrained Active Power Curtailment Considering Line Temperature and Therma...
Roman Bolgaryn
Jan Wiemer

Roman Bolgaryn

and 3 more

June 12, 2023
We introduce a novel method to determine cost-optimized Active Power Curtailment (APC) of Renewable Energy Sources (RES) considering weather dependent Dynamic Line Rating (DLR). A new formulation of the Security-Constrained Optimal Power Flow (SC-OPF) is developed and applied in a case study. We demonstrate the reduction of the required preventive APC in the (N -0) case for (N -1) secure grid operation due to the consideration of thermal inertia of overhead power lines. Considering thermal inertia allows relying on curative APC that is activated only after an (N -1) situation occurs. The overhead power line temperature, calculated with the temperature dependent power flow, acts as a new limit for line loading and releases unused transmission reserves. The power system can be utilized to a greater extent, with a reduced demand for congestion management.
Rapid identification of three species of Schisandra chinensis based on UPLC-Q-Orbitra...
Yubo Li
Lexin Shu

Yubo Li

and 8 more

June 10, 2023
Rationale: Schisandra chinensis is a traditional Chinese medicine in China. Based on the ultra-performance liquid chromatography quadrupole-Orbitrap-mass spectrometry (UPLC-Q-Orbitrap-MS) technique, we rapidly identified the components of Northern Schisandra chinensis(SS), Schisandrae Chinensis Fructus processed with vinegar(CS), Wine-processed Schisandra chinensis(JS), and distinguished the differences of the chemical composition of the three Schisandra chinensis before and after processing, to provide a reference for rational clinical use. Methods: In this study, used the UPLC-Q-Orbitrap-MS and data post-processing techniques. Based on the literature summarizing the fragmentation information of possible characteristic fragments(CFs) and related neutral losses (NLs)of lignans, organic acids, flavonoids, terpenoids and other components of Schisandra chinensis, then by comparing with the mass spectrum data obtained from the detection, to achieve rapid classification and identification of SS, CS and JS. Results: In this study, 51, 41 and 42 compounds were successfully identified from SS, CS and JS, respectively. The composition of Schisandra chinensis differs before and after processing, the realization of the distinction between the three traditional Chinese medicines. Conclusions: In this study, to distinguish SS, CS and JS in terms of chemical composition differences. It can achieve the rapid classification and identification of different processing technology of Schisandra chinensis and provide a reference for safer and rational clinical application.
ON THE ORIGIN OF KANIV DISLOCATIONS IN CONNECTION WITH THE PROCESSES OF FORMATION OF...
Anatolii Karpenko

Anatolii Karpenko

June 20, 2023
A.M. Karpenko, 2022Institute of Geological sciences of the National Academy of Sciences of Ukraine, Kyiv, UkraineWeak links in the existing models of Kaniv dislocations formation are shown. A new concept of a complex of factors, conditions and processes of paragenesis of buried overdeepened depressions and glaciodislocations of the Kaniv type in continental glaciation areas has been proposed and substantiated. The theory of glacioisostasis is involved to the construction of the glaciotectonic model, in particular, the theory’s statement about occurrence of a temporary compensatory glacioisostatic elevation of relief in the form of a roll before the glacier front, subparallel to its edge. In the core of the roll rocks, previously buried at a considerable depth, will be elevated above the local erosion base. When the glacier’s movement slows or stops, its melt water flowing out from the periglacial lake through the low section of the roll crest, as the roll rises, will develop an antecedent valley across the roll - a breakthrough with steep slopes, cutting through the rocks raised from the depths. As the glacier accelerates and reaches the roll, this breakthrough gives the glacier tongue the shape of a wedge (in the horizontal projection). With further increase in pressure of the ice mass glacier wedge with its lateral surface tears off the rock blocks from the slopes of the breakthrough and moves them forward and to the side. Subsequently the compensatory roll is sinking (is smoothed out), and the erosion-exaration breakthrough turns out below the level of the local erosion basis, is buried and transforms into an overdeepened depression.Key words: glaciodislocations; overdeepened depression; glacioisostasis; compensation roll; erosion; breakthrough; glacial wedge; exaration.Introduction. The Kaniv mountains are a unique natural object with a mysterious origin, which has been discussed by geologists, geographers, and naturalists for more than a hundred years. These mountains are composed of crumpled, deformed layers of rocks that lie at depths of up to a hundred metres around them, and in the mountains are raised more than three hundred metres upwards. More than a hundred scientific papers have been devoted to these dislocations and the mechanism of their formation, and dozens of versions of how they were formed have been proposed by researchers.Almost from the very beginning of the stage of detailed geological study of the Kaniv dislocations in the first half of the last century, their genetic connection with another outstanding geological object discovered nearby - a depression buried under thick Quaternary sediments in the bed of Paleogene and Mesozoic rocks, later named by G.I. Horetskyi [1970] Shevchenko Scour of glacial ploughing and glacial erosion - became clear.In our opinion, the key to reconstructing the mechanism of formation of the Kaniv dislocations can be found in an adequate explanation of the genesis of the so-called ”overdeepened valleys” (overdeepened depressions) of the Shevchenko type.Buried overdeepened valleys of the Russian Plain, concepts of their formation. The widespread occurrence of deep scouring in the glacial region of the European part of the USSR was noted by B.L. Lychkov [1942]. He considered the fragments of buried valleys discovered here to be traces of an ancient (pre-Quaternary) buried river network and even tried to reconstruct it. Initially, other researchers believed the same, supplementing the picture of the distribution of fragments of buried overdeepened valleys with new factual data, for example, E.V. Rukhyna [1957]. This point of view prevailed for a long time. But eventually, based on the data of detailed drilling coverage of the areas of development of such valleys, G.I. Horetskyi [1967, 1973] showed one of the most important features of their structure - they turned out to be elongated but closed.This is the first argument for our version of the mechanism of their formation.Other arguments are as follows.Deep valley-like depressions in the relief of the Quaternary sediment bed are common also in watersheds and watershed slopes, where their depth reaches 300 m; the location of valley-like depressions in the plan view in most cases does not correspond to the modern pattern of the hydrographic network [Horetskyi, 1967].In many cases, the bottom of these valleys is located tens or even hundreds of metres below the modern sea level.G.I. Horetskyi [1967] noted a peculiarity of some of these depressions: the presence of narrow canyon-like, as he calls them, ”gullies of glacial runoff and erosion” at their base. They are several tens of metres deep below the moraine’s sole and are filled mainly with sands, sometimes with gravel and pebbles.The cross-sectional profile of the valley-like depressions is V- and U-shaped (”gully” and ”trough” types of glacial depressions respectively - [Horetskyi, 1980]); the slopes are steep, sometimes steep-to, and the width of the valleys is small (E.V. Rukhyna [1957] in the Moscow area indicates a width of 1 to 2.5 km).E.V. Rukhyna [1957] notes the typical properties of the Quaternary sediments that bury the valleys. Glacial formations account for the largest share in their composition. The lower moraine (there are up to three horizons) usually lies on bedrock, and there is no thick complex of alluvial deposits beneath it (gravel and pebble underlying sands of low thickness are found). The absence of alluvial deposits at the bottom of overdeepened valleys is also confirmed by G.I. Horetskyi [1967]. He also draws attention to the almost universal accompaniment of deep valley-like depressions by glaciodislocations, by inclusion in the moraine of numerous detached masses, ranging in size from small lumps to large blocks with a diameter of several tens of metres [Horetskyi, 1968, 1972].The features pointed out by E.V. Rukhyna indicate the formation of valleys as a result of vigorous rapid incision caused, as she believes, by uplift of the territory; active erosion continued until the very beginning of glaciation. After that the territory was levelled.Based on extensive factual material, G.I. Horetskyi [1967] denies the purely erosive origin of the overdeepened valleys, especially since they turned out to be closed depressions rather than buried river valleys, and proposes a version of their formation by the joint action of glacial exaration and glacial erosion.The presence on modern watersheds and valley slopes of numerous and huge erratic masses and dislocated massifs involving those ancient rocks which lie at considerable depths in the glaciation zone (and which, however, are reached by nearby overdeepened depressions) indicates that masses of these rocks were torn away near the bottom of the depressions and brought to the surface by some powerful force.The history of the discovery and study of the Kaniv dislocations and the Shevchenko Basin shows that initially researchers quite logically linked such grandiose phenomena to tectonic processes. However, the participation of glacial formations in the structure of the sedimentary strata, that fills the overdeepened depressions, gave rise to the version of another group of researchers - about the purely glacial origin of the overdeepened depression (Shevchenko), and therefore, the related with it Kaniv dislocations. What is obvious in this version is the exaggeration of the bulldozing (ploughing) capabilities of the glacier, attributing to the plastic-viscous movement of the glacier on flat terrain a dubious ability to have a powerful vertical component: at first wedge in into the bedrock to a considerable depth, and then emerge again to the surface, and even with huge masses of rock removed from depths of a hundred metres.  (A.V. Matoshko and Yu.G. Chuhunnyi [1993] estimate the normal depth of exaration of the Dnieper glacier to be a maximum of several meters, and the incredible depth of exaration in the Shevchenko Basin they call apotheous, without explaining the reasons for this anomaly). That is why one more the concept - of the two-factor origin of the Kaniv and other dislocations appeared. D.K. Bilenko [1939] mentioned the position of V.M. Chyrvynskyi on these issues, who simply and accurately stated that for glaciated dislocations to occur, one must assume the existence of an uplift within the moraine terrace before the glacier arrived; otherwise, the appearance on the flat terrain of such isolated hills through dislocating by glacier becomes unclear.However, after further research failed to confirm the existence of tectonic structures in the Kaniv area, only the glacial component of the hypothesis remained valid and the hypothesis’ persuasiveness was reduced. In addition, M.H. Kostianoi [1962] carried out engineering and geological calculations of the tangential forces of the glacier in the Kaniv area. "The results of calculations show that the hypothesis of glaciodislocations in an elementary form does not find justification" - came to the conclusion of M.H. Kostianoi [1962, p. 81]. Feeling this flaw of the ”glacial” remnant of the hypothesis of the formation of a paragenetic pair "depression-dislocations", researchers began to add to it versions of various processes:  washing out of rock from under the glacier by subglacial waters; freezing of substrate blocks to the glacier sole and their subsequent movement to the surface together with the glacier; extrusion of incompetent substrate material into various cavities in the glacier body; squeezing of rocks, that have acquired a plastic consistency, from under the glacier edge under the weight of the glacier, etc. For example, the authors of the most recent special study of the Kaniv dislocations opted for a model of their formation that assumes glacial pressure on the substrate to be so great that it caused ”active progressive movement of the rocks of the glacial bed under the influence of glacial loads” [Lavrushyn and Chuhunnyi, 1982, p. 92]. (Here Y.A. Lavrushyn and Yu.G. Chuhunnyi [1982] resorted to the model proposed by F. Wahnschaffe [1882] 100 years before - the circle is closed). But along the entire glacier path, at least along most of it, the geological conditions of the glacier bed often change only slightly, so this mechanism of glaciodislocations formation would have resulted in their almost continuous presence along most of its path; however, instead, we have a very limited distribution their in some relatively small segments of this long line - and this selectivity, having no explanation, makes the Wahnschaffe-Lavrushyn-Chuhunnyi version unconvincing. Also and other additions did not help to create a coherent and convincing model of the paragenesis of such objects as the Kaniv dislocations and the Shevchenko overdeepened depression. And the same weakest link of all concepts is due to the search for a version of the mechanism within the framework of an unchanged postulate: a huge mass of rocks (up to 50 km3 in volume - [Matoshko and Chuhunnyi, 1993]), which was clamped on all sides at a depth of more than a hundred metres, was torn off and raised to a height of about two and a half hundred metres. One of the researchers wrote in this regard: ”It is known that the specific gravity of ice is less than 1 kG/dm3, while the specific gravity of sedimentary rocks is on average 2.5 kG/dm3. Thus, in order to compensate for the 270 metre rise in rock, the ice cover would have to be 675 metres high, and in a small area. But even if this condition was met, it is impossible to imagine how the glacier, whose bed was at levels close to 100 m above sea level, in a strip up to 10 km wide, plunged and tore a piece of rock from a depth of 130 m. It is unclear what forces must have acted to break and move the huge mass. And these two stages require maximum effort, since not only the force of inertia but also the force of adhesion is at work here” [Pazynych, 2007, p. 196]. In his major work [1970], G.I. Horetskyi in the most detailed way considers the history of research, structure, history of formation and development of the Shevchenko basin of ”glacial ploughing and erosion” but avoids considering the mechanism of formation of the Kaniv dislocations. However, due to the genetic relationship between the Kaniv dislocations and the Shevchenko basin, there should be a single convincing version of the mechanism of their joint formation. G.I. Horetskyi apparently was sensing that his hypothesis of glacial ploughing and glacial erosion was not such.Thus, the main ways of glaciodislocations formation are now considered to be: disruption and movement of large blocks of rocks under the pressure of an advancing glacier (”bulldozer” effect, or pushing); squeezing of pliable rocks from under the glacier edge - squeezing; injection of loose plastic material into cavities in the ice thickness [Ananiev et al., 1992].Compensation roll as a key node in the concept of the paragenesis of glaciodislocations and overdeepened depression. Researchers in vain have completely rejected the tectonic component of the hypothesis of the complex origin of the Kaniv dislocations and the Shevchenko overdeepened depression.None of them, from the beginning of the research to the present day, has been able to assume that the tectonic uplift in the Kaniv area could have been temporary and not left any distinct traces in the earth’s crust. For example, S.L. Breslav wrote [1971, p. 636]: ”At the same time, it is difficult to explain such a significant deepening by water erosion alone. In this case, it is necessary to assume either an extremely sharp decrease in the overall erosion base or unusually large amplitudes of tectonic uplifts, which for some reason were subsequently replaced by equally powerful movements of the opposite sign. Both assumptions do not currently have any reliable justification”.The latter is not true. At that time the theory of glacioisostasy already existed, which describes tectonic movements just of this nature.The idea was proposed by T.F. Jamieson [1865] in the second half of the 19th century, and in the first third of the 20th century it was already widely accepted, and it is somewhat surprising that even in later times, domestic researchers of the Quaternary continental glaciation of the East European plain did not widely apply its provisions (only V.V. Riznichenko in his works of the early 1930s pointed to the possibility of oscillatory movements of the crust associated with glacial loading and unloading; some attempts to apply the theory of glacioisostasy to explain some features of the relief structure of glacial and extraglacial areas can be found in the works of B.L. Lychkov [1927, 1928, 1931, 1942, 1944]).One of these provisions is the phenomenon of the earth’s surface uplift zone (compensation roll) along the leading edge of the glacioisostatic deflection area. As early as 1874, N.S. Shaler [1874] suggested the existence of such a phenomenon as a consequence of squeezing out from under the glacier of plastic substance in the earth’s shell, which lies below the crust. Later, this concept was developed by F. Nansen [1922, 1928] (who noted that after deglaciation the roll-like uplift should sink) and R.A. Daly [1934]. To a certain extent, the theoretical position on the compensatory glacioisostatic roll was developed in articles by B.L. Lychkov and in monographs [Nazarov, 1971; Levkov, 1980].After a number of investigators in 60-80s of the last century managed to establish geological and geomorphological evidence of existence of compensating rolls in glacial regions of the North America (Anderson R.C.; Bloom A.L.; [Newman et al, 1971]; Walcott R.I.), northern-western Europe ([Boillot, 1964]; Emery K.O., Aubrey D.G.; Newman W.S. et al.; Mary G.; Mörner N.-A. et al.) and the European part of Russia (Bylynskyi E.N.), E.N. Bylynskyi stated in his monograph [1996, p. 138]: ”In accordance with modern concepts, during ice accumulation, large deflections occurred in the ice sheet bed under the influence of increased loads. These deflections spread for several hundred kilometres along the periphery of the glaciers within the platforms and in the periglacial zone, causing here the formation of a flexure sloping towards the ice sheet. In the distal direction from the glacier, it ended with a roll-like uplift (advanced roll). The amplitude of the uplift seems to have varied from place to place, depending on the structural features of the lithosphere, but according to many researchers, it could have been 50 – 200 metres”. The author substantiates also by the results of his own 30 years research that ”With each major glaciation, under the influence of elastic stresses and as a result of the substance movement asthenosphere from the area of formation of the glacier during its growth and in the opposite direction with his degradation, on the periphery of the ice sheets were raised and disappeared roll-shaped elevations up to 200 m. Together with flexure-like tilts of the lithosphere they had a significant impact on sedimentation and formation of the relief of the periglacial zone several hundred kilometres wide” [Bylynskyi, 1996, p. 166].In the new XXI century, researchers (for example: J. Wallinga; F.S. Busschers et al. [2007]; A. Panin et al. [2015]; N.N. Nazarov, S.V. Kopytov [2020]; A.O. Utkina [2020]) often use the concept of the formation of a temporary roll-like uplift in front of the glacier - a glacial forebulge - in reconstructing the history of development and explaining the structure of river valleys in the periglacial zone.Already in the last century, the development of mathematical models of the phenomenon of glacio- and hydroisostasis was initiated and continued in the new one (for example, Peltier W.R. and colleagues). The modeling confirms the bending of the lithosphere under the weight of the covering glacier and the compensatory rise at a certain distance from it.Geophysicists joined the development of the glacioisostasy theory shortly after the idea emerged. One of the first was N.S. Shaler, who in 1874, analysing glacioisostatic phenomena, predicted the existence of the asthenosphere, explaining by the properties of this earth layer also and the formation of a temporary compensatory roll around the ice sheet. Among the geophysical studies of glacioisostasy in the next XX century, we can mention the works of B. Gutenberg (first half of the century), R.I. Walcott (70s).E.V. Artiushkov [1967, p. 14] attributed a significant role to the phenomenon of compensation roll formation: ”When a glacier advances, the asthenosphere material squeezed out from under it leads to the formation of a gentle rise 100-200 m high outside the glacier when an average glacier thickness of 2-3 km. This rise on the flat territory actually forms a new watershed and causes flooding of large areas between the glacier, the rise and the uplands located perpendicular to the glacier edge”. Other geophysics S.A. Ushakov and M.S. Krass [1969] estimate the magnitude of such uplifts to be much smaller: a few, at most, the first tens of metres. However, these authors also admit that in the presence of areas of hardening and/or increased asthenosphere viscosity crustal uplifts of hundreds of metres are possible between each of them and the glacier edge.N.F. Balukhovskyi [1968, 1973] came close to the idea of the formation of a temporary uplift in front of the glacier. However, in his version, the periglacial gentle roll (40-50 m high, 5 to 20 km wide and several tens of kilometres long) was formed by plastic Jurassic and Triassic clays extruded from under the glacier (in fact, this construction by N.F. Balukhovskyi is "squeezing"). In addition to the fact that the formation of the roll in this way is somewhat problematic, Balukhovskyi’s description of the further development of the situation is, in our opinion, not convincing. To explain the occurrence of dislocations, the author uses seismic waves, which, in his opinion, were caused by shocks emanating from the glacier and activated the plastic Jurassic clays of the periglacial roll, and these clays introduced in the cover stratum, what resulted in the formation of a system of folds-upthrows. No explanation is given as to why the glacier did not push back (did not ”roll”) the roll further forward or did not flow over it, but, instead, began to cut away it, and under the glacier at the same time ”a depression of detachment up to 150-160 m deep was formed and gradually expanded”.The role of glacial lakes. Geological evidence for the extensive development of lake basins in front of the glacier edge is often described in the literature (J. Mangerud et al.; A. Panin et al.; D.D. Kvasov; E.N. Bylynskyi; N.N. Nazarov and S.V. Kopytov). The formation of glacial lakes reconstructed on the Russian Plain was explained by A.S. Lavrov and D.D. Kvasov [1963, 1975], and in Western Siberia - by I.A. Volkov et al. and S.A. Arkhipov et al. by the damming of river valleys by the glacier. In the becoming of the glacioisostasy theory another condition for the formation of periglacial lake basins was stated: in the band of undeglacier glacioisostatic depressions that extended beyond the glacier along its edge [Bylynskyi, 1975, 1996]. This method of lake formation was greatly facilitated by the uplift of the compensation berm [Artiushkov, 1967], which could provide a great accumulation of glacier meltwater with a sizable excess of its level over the local erosion base, because such lake basins received high banks: the glacier slope and the opposite slope - of the compensation roll.The factor of breakthrough valleys. The phenomenon of the formation of drainage channels (glacial lake spillways), that discharge water from overflowing glacial lake basins, has long been established, and researchers are discovering new and new forms of this type of relief (e.g., J. Mangerud et al.; A. Panin et al.; [Nazarov and Kopytov, 2020]).Water discharge usually starts through a saddle in a watershed with a neighbouring river basin and becomes catastrophic. Spillways often are large in size and have steeply slopes, and the largest of them (kulli´) are canyons that reach a depth of about a hundred metres, cutting through and through the watershed massiv.From the descriptions of buried overdeepened depressions, summarised in the literature (see above), it is clear that they are morphologically very similar to spillways. This circumstance, as well as the distribution of buried overdeepened depressions exclusively within or close to the area that in past was covered by glaciers, leave no doubt about their genetic correspondence to spillways. The difference from the spillways lies in the burial of the depressions by Quaternary sediments, the location of their bottoms well below the local erosion bases, and the arcuate concavity of this buried bottom.Glacier bulldozing effect. On flat terrain, there are no traces of significant exaration (in the sense of denudation of the glacier bed) activity of a glacier, for example, the Dnipro glaciation [Matoshko and Chuhunnyi, 1993]. This is explained by the visco-plastic nature of ice flow under the influence of its own weight [Kapytsa, 1958]. However, at certain stages of research on the mechanical impact of glaciers on the substrate, some researchers considered this impact to be significant. ”Based on the relationship between detached masses and erosion glacial forms, a number of researchers do not exclude that in some areas the glacier acted on the bed like a giant plough: it plowed out the troughs, deformed the sediments in their sides, and threw material onto the slopes.” [Levkov, 1980, pp. 27-28]. And it would seem that such objects as the Kaniv dislocations can confirm this point of view. However, the fact that a long range of versions of their formation mechanism have been proposed indicates that this old concept - the plow out of huge masses of rock by a glacier from depths of hundreds of metres (see above) - is not convincing to many researchers.In this regard, it is worth mentioning that as early as 1880 H. Credner [1880], using examples from northwestern Saxony, concluded that dislocations in boulder clay layers were caused by lateral (tangential) glacial pressure, and that the formation of dislocations was facilitated by the presence of surface irregularities in the substrate.Indeed, if the ice flows under the influence of its own weight, the ”pivotal” level of this flow must lie in the lowest layers of the continental glacier thickness, where the vertical weight vector of a huge ice thickness is transformed into a horizontal one. Therefore, in the glacial tongue slopes (both frontal and lateral), the lower ice layer permanently push forward, playing the role of a ”knife” that can ”cut away” uneven terrain, especially with a steep slope that is towards to the glacier movement. Glacier movement does not stop if, after the ”knife” is extended, the entire ice column immediately moves over it - this maintains pressure on the lower layer. This also implies that the slopes of a glacier advancing across flat terrain must be very steep. So, indeed, the effect of a ”mould blade of bulldozer” many hundreds of metres high is created, which can undercut and displace elevated landforms.But even if such a mechanism does work, it does not at all provide the possibility of lifting to the surface of rocks lying at great depths under the glacier.Discussion. And here we need to return to the question of the mechanism of formation of the overdeepened depressions.The flow of viscous matter in the upper mantle, caused by the glacial load, being inert in nature, obviously lagged behind the advance of the glacier edge - but this advance could not but be uneven. It is quite logical that during the phases of the glacier suspension, the wave of subcortical stresses could have time to "roll out" from under the edge of the glacier in the form of a compensation roll. At the same time, the waters of the near-glacial lake could overflow the bowl of the lake basin and to found in the compensatory roll the lowered areas of its ridge, through which they overflowing to its other side. The slope of this flow was significant. Intense erosion formed a narrow, deep spillway in the roll (an analogy is the breach of a pond dam), at the bottom of which alluvium was not deposited, or was deposited already only at the stage of levelling by erosion the longitudinal profile of the spillway (see above description of the properties of buried overdeepened valleys). This process could accompany the growth of the roll from its very beginning, in particular along the valley of the already existing runoff in the direction from the glacier - an antecedent erosion segment could gradually form within the roll. And this spillway, even if its bottom remained slightly higher than the general surface level outside the roll, in roll would cut through rocks that lie at a considerable depth outside it. Within a short (on geological scales) time of 103-10* years (Artiushkov, 1979) the restoration of isostatic equilibrium (relaxation) take place. And the further fate of the spillway is to be lowered (together with the compensatory roll, which isostatic sinking, including under the pressure of ice masses if the glacier continued actively to advance), and later, in the process of glacier melting, to be filled with glacial formations, then lake and other sediments and become a buried depression of an elongated closed shape. The longitudinal profile of the bottom of an erosion form in a roll, close to a straight line, becomes concave after it sinking - this arcuate shape is noted by researchers when studying the morphology of buried overdeepened depressions.In our opinion, this is how buried valley depressions were formed in the Quaternary glacial zone of the Russian plain.If the glacier accelerates its advance at the mature stage of formation of a spillway in the compensatory roll, then, having reached the roll, the visco-plastic ice flow will find a breakthrough in it, in which will inevitably form an ice wedge. The concept of an ice wedge is found in the works of researchers. However, they used it rather to give the text an emotional colouring: ”This is the moving huge mass that formed a living and plastic wedge driven into the Dnieper valley” [Soboliev, 1926, p. 205]; ”The Dnieper glacier invaded the Roslavl-Seshchyn combe in a powerful wedge, crushed the underlying interglacial deposits (especially clays), deformed the combe sides, captured many detached masses and deposited in the depression moraine deposits up to 25-35 m thick, and in places (Seshchynskaya station) up to 60-65 m thick” [Horetskyi, 1967, p.22]; the wedging role of the glacial tongue in the formation of the Seshchinskaya glaciodislocations somewhat more definite was stated by D.I. Pohuliaiev [1956, 1968, 1972]. We give this notion a key meaning in our concept, and we mean the wedge-shaped shape of the glacial tongue not in vertical section but in plan, because if the ice wedge is directed downwards with its ”tip” (wedge-shaped in vertical section), the lateral surface of such a wedge does not exert a direct upward squeezing force on the contact rocks but only a very indirect one through down and side pressure on these rocks. And in the case of pressure on the rocks of the erosion scarp of the spillway in the compensation roll, it is enough for the ”horizontal” ice wedge only to move the substrate rocks, that have already been raised and even exposed by erosion in the precipice of the spillway. The onslaught of ice only needs to overcome the forces of adhesion and internal friction of the rocks, and throw them forward, sideways and downward (to the distal foot of the roll), where the pressure vector of the lateral sub-vertical surface of the ”horizontal” ice wedge in the deep, steeply sloped compensation roll scour is directed.This situation greatly enhances the dislocation capabilities of the glacier. By wedging the scour further, as if opening the gates wider for the main ice masses, the ice wedge moves the rocks, that component the scour sides, forming dislocations. The glacier’s task of destroying the sides of the scour was further simplified by the fact that, bending into the shape of a roll, the rock layers lost some of their strength (are decompacted and cracked); in addition, clay rocks could acquire a more plastic consistency.This process continued until the main masses of ice arrived, when they began to overflow through the compensation roll.The idea of the horizontal wedging effect has obviously occurred to researchers many times before. However, within the framework of the same approach (mobilisation of large masses of rock deep in the sedimentary series), it gave little for insight into dislocations such as those in Kaniv.By the end of the last century, discussions about the mechanism of formation of the Kaniv dislocations freeze. The scientific thought stopped at the glacioextrusive concept [Lavrushyn and Chuhunnyi, 1982] - squeezing . There are no grounds to deny the involvement of glaciodiapirism and glacioextrusions in the formation of the Kaniv dislocations, but it seems that these processes played a far from major role. Researchers note the manifestations of diapirism in other similar depressions to the Shevchenko but do not assign it a leading role in the formation of the corresponding forms.The authors of the most recent thorough study of the Kaniv dislocations stated: ”Thus, when creating the concept of the origin of the Kaniv dislocations, one has to take into account many factors, not just one of them. Apparently, the underestimation of this circumstance can be explained the current lively discussion about the origin of the Kaniv dislocations and the obvious inconclusiveness of both glaciotectonic and purely tectonic constructions” [Lavrushyn and Chuhunnyi, 1982, p. 84].Fully supporting this conclusion of these and other researchers, we argue from the standpoint of a systemic paleogeographic approach that the complex multifaceted phenomenon of the formation of two genetically related objects (the overdeepened valley and glaciotectonic dislocations adjacent to it) requires a favourable combination of a number of factors and of specific phases of their dynamics.To begin with, we point out the need for a geological structure that is favourable in this area for the formation of a sufficiently high compensation roll.Further, much depends on the duration of the glacier’s suspension phase: it must be optimal, i.e. sufficient not only to form a compensation roll but also to create a deep and sufficiently wide spillway in it, otherwise the glacier will simply ”flow” over the roll; and if the glacier is stopped for too long, the roll will begin to flatten out, the bottom of the spillway will sink downwards, glacioaluvium will appear on him, and a flowing lake basin will eventually form, which will be filled with sediments. In the latter casen an overdeepened depression will form (the ”gully” type of glacial depressions - [Horetskyi, 1980]) but large-scale dislocations are unlikely.If the gully does not cut through the roll to its base, there will be no alluvium in it, and moraine will lie down on the substrate rocks at the bottom of the prepared by the glacier exaration gully (”trough” type of glacial depressions - [Horetskyi, 1980]).These simplified reconstructions of ours present only a few of the many possible combinations of the features and dynamics of the main factors and conditions of the phenomena discussed in this article. That is, the variability of the specific consequences of the described processes is quite wide. Therefore, not many overdeepened depressions have been found in the wide areas was covered by Quaternary glaciers, probably their number is in the tens; and even fewer large glaciodislocations are found near them.An interesting question is the size of the compensation roll.E.V. Rukhyna [1957] suggests that the formation of buried overdeepened valleys of the glacial zone required before glaciation the uplift of the territory by several hundred metres. Considering the same issues in major monograph [Markov et al., 1965], K.K. Markov agrees that the pre-glacial surface of the Russian plain exceeded its present-day surface by about 400 m. These values, in fact, can be considered to characterise the range of heights of compensation rolls, the formation of which was accompanied by vigorous cutting through by glacial meltwater. The estimate of 400 m is probably too high, as it was given by the authors who considered the overdeepened valleys to be part of the pre-glacial river network and, therefore, linked this value with the general erosion base, while only the local base should be taken into account. Therefore, when assessing possible roll heights, in each case it is need to start from the depth of the buried depression: the height of the roll above the surrounding surface should have been no less than the depth of the gully that cut through it. Whether this depth corresponds to the maximum height the roll reached remains unknown.The literature contains empirical data on the height of the compensation roll. For example, G. Boillot [1964] calculated the height of the roll in water area of the English Channel in the Late Valdaian time - it reached at least 70 m. Studies by W.S. Newman et al. [1971] on the US Atlantic shelf showed that the height of the roll in the Late Wisconsinan could exceed 80 m.The length of the buried trough also indicates the width of the roll. This estimate is very approximate, as several unknowns factors are involved: whether the erosion of the roll occurred perpendicular to the roll axis or at an acute angle (up to subparallelism to the glacier front); at which dynamic phase of roll formation the erosion cutting stopped; and as the roll moves, the length of the gully may exceed roll width.Conclusions. Attraction of the glacioisostatic theory principle on the possibility of formation of a temporary compensation roll in front of the glacier allows us to construct a concept of the paragenesis of glaciodislocations such as the Kaniv and the overdeepened depressions, that is free from weak link in the previously proposed reconstructions: problematic of detachion at great depths and the high uplift of significant masses of rock by the glacier. It is quite logical that only a tectonic factor can cause such an uplift.Another condition for the formation of both the overdeepened depressions and glaciodislocations is the development of a breakthrough across the roll during the slowdown (suspension) of glacial advance. Finally, the third condition is the timely resumption of glacier movement, which must have time to wedge into the breakthrough before the compensatory roll is sinking (flattened).As the pressure of the ice mass continues to increase, the ice wedge by lateral surface destroys the sides of the gulley, detached blocks of rocks from them and pushed these blocks forward-side-down, thus forming the glaciodislocations. Subsequently the compensatory roll was sinking (smooth down) and the erosion-exaration scour, that formed in it, had found itself below the level of the local erosion base, was buried and became an overdeepened depression.The uniqueness of the pair of geological monuments of the modern nature of the Dnipro region - the Kaniv mountains and the Shevchenko buried overdeepened valley - is undoubtedly due to a very favourable combination of the many circumstances and factors, described above, and of their dynamics during one of the episodes of glacial transgression.We should also note that the palaeogeographic reality of the formation of a temporary glacioisostatic compensation roll in front of the glacier is strongly being confirmed by the eloquent fact of the existence of overdeepened depressions. After all, the only realistically possible agent for creating these landforms, which were subsequently buried, is erosion, and erosion can act only above its local base. Even if we assume that the buried depression was created as a tunnel (by subglacial pressure flows), the explanation of the next, main stage of dislocation formation - the uplift of huge masses of bedrock from a considerable depth - will remain very problematic without the factor of compensation roll.
INCIDENCE AND RISK FACTORS FOR UMBILICAL CORD PROLAPSE WHEN AMNIOTOMY IS USED: A SWED...
Sofia Tallhage
Kristofer Årestedt

Sofia Tallhage

and 3 more

June 10, 2023
Objective: This study aimed to examine incidence for umbilical cord prolapse, and to explore risk factors in labours where amniotomy is used. Design: Retrospective nationwide register study. Setting: Delivery wards in Sweden included in the Swedish Pregnancy Register. Participants: All births from January 2014 to June 2020, eligible participants n = 607 123. Methods: The main outcome, umbilical cord prolapse, was identified in the data by the International Classification of Diseases (ICD-10), diagnosis code O69.0. Multiple binary logistic regression analysis was used to identify risk factors. Results: Amniotomy was performed in 230 699 (38.0%) of the births, in which umbilical cord prolapses occurred in 293 cases (0.13%). Spontaneous rupture of the membranes occurred in 376 424 (62.0%), in which there were 458 cases (0.12%) of umbilical cord prolapse. Risk factors associated with umbilical cord prolapse in labors where amniotomy was used were higher parity, previous cesarean section, presence of polyhydramnios, induction of labour and non-cephalic presentation, that all increased the odds for umbilical cord prolapse. Conclusions: The severe complication of umbilical cord prolapse is rare in Sweden. Identified risk factors in labours where amniotomy is used were higher parity, previous cesarean section, polyhydramnios, induced labour and a baby in a non-cephalic presentation. Funding: Linnaeus University and LÖF; the Swedish patient insurance. The funders had no role in study design, analysis and interpretation of the data, or writing the article.
On the electron impact cross-section maxima for dissociative and non-dissociative sin...
Antônio Santos

Antônio Santos

June 10, 2023
Determining absolute electron impact ionization cross-sections for atoms and molecules, whether through experimental or theoretical means, poses a formidable challenge and is typically limited to a narrow selection of species. In this paper, by using measured cross-sections and polarizability data, it was obtained empirical scaling laws for dissociative and non-dissociative single ionization cross-section maximum of neutral molecules by collisions with electrons. It is found that the dissociative cross sections scale linearly with the target electric static polarizability, while the non-dissociate cross sections exhibit a saturation effect for large target polarizabilities. This saturation effect is interpreted as the increasing role of fragmentation with polarizability.
Development of an analytical method for the determination of pesticides in tropical f...
Sabriye Sel
Elif Öztürk Er

Sabriye Sel

and 2 more

June 10, 2023
Rationale: Pesticides are poisonous substances or mixtures of dangerous chemicals used to prevent, dissuade, control, or eradicate insects, weeds, rodents, fungi, or other potentially dangerous organisms. Pesticides must be separated from complex matrices before analysis by performing the proper extraction, cleaning, and/or preconcentration processes. The QuEChERS method served as a matrix clean-up tool and the DLLME method preconcentrated the analytes for their determination at trace levels. To increase the detection efficiency, a simple and efficient pretreatment process is required. Method: In this study, QuEChERS extraction was combined with dispersive liquid-liquid microextraction (DLLME) to extract pesticides from tropical fruits for determination by a highly accurate and sensitive liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS) system. All the variable parameters of the DLLME method were optimized to improve the extraction output for all analytes and the limits of detection and quantitation (LOD and LOQ) values, which are quite satisfactory, were calculated under the optimum conditions. Results: The LOD and LOQ values were found in the range of 0.004 – 0.013 and 0.27 – 0.61 µg/L, respectively. The detection limits achieved by direct LC-QTOF-MS/MS analysis were increased by about 10 – 260 fold using the optimized DLLME method. To assess the accuracy and applicability of the developed method, recovery experiments on tropical fruits were carried out. The matrix-matched calibration method was used to enhance the quantification accuracy of the analytes in kiwi, pineapple, and mango matrices, with percent recoveries ranging between 89 and 117%. Conclusion: The results show that the proposed method is feasible for the determination of pesticides with high accuracy and precision. The above approach acts as a reference method for monitoring tropical fruits while proposing prospective solutions for pesticides assessment in more complex matrices.
Diagnostic performance of a fully automated hepatitis E virus antibody immunoassay
Andi Krumbholz
Anna Eichhorn

Andi Krumbholz

and 7 more

June 13, 2023
In immunocompetent patients with liver inflammation, the detection of IgG and IgM antibodies against the hepatitis E virus (HEV) is important for the diagnosis. Recently, fully automated chemiluminescence immunoassays (CLIAs, LIAISON® Murex anti-HEV IgG/anti-HEV IgM test, DiaSorin) have become available for this purpose. The diagnostic suitability of these CLIAs was determined by comparison with a combination of plate-based enzyme immunoassays ( recomWell HEV IgG/IgM ELISA, Mikrogen) and immunoblots ( recomLine HEV IgG/IgM, Mikrogen), which served as a reference for the characterization of sera. Samples with a deviating result were retested with an alternative test (WANTAI HEV IgG/IgM ELISAs). The anti-HEV IgG CLIA had a sensitivity and specificity of 100% (100/100; 49/49) each when the recomWell HEV IgG ELISA served as a reference. The anti-HEV IgM CLIA had a sensitivity of 67.9% (36/53) and a specificity of 100% (49/49). When IgM immunoblot results were considered, sensitivity and specificity were 88.9% (24/27) and 53.8% (14/26), respectively. The WANTAI test confirmed 52.9% (9/17) of negative CLIA IgMs that differed from the recomWell HEV IgM result. The CLIA revealed an isolated and thus probably non-specific HEV IgM in one of 17 patients with acute Epstein-Barr virus infection. The automated CLIAs are well suited for HEV diagnostics.
FMCW Radar on LiDAR Map Localization in Structual Urban Environments
Yukai Ma
Han Li

Yukai Ma

and 6 more

June 10, 2023
Multi-sensor fusion-based localization technology has achieved high accuracy in autonomous systems. How to improve the robustness is the main challenge at present. The most commonly used LiDAR and camera are weather-sensitive, while the FMCW Radar has strong adaptability but suffers from noise and ghost effects. In this paper, we propose a heterogeneous localization method called Radar on LiDAR Map (RoLM), which aims to enhance localization accuracy without relying on loop closures by mitigating the accumulated error in Radar odometry in real time. Our approach involves embedding the data from both Radar and LiDAR sensors into a density map. We calculate the spatial vector similarity with an offset to determine the corresponding place index within the candidate map and estimate the rotation and translation. To refine the alignment, we utilize the Iterative Closest Point (ICP) algorithm to achieve optimal matching on the LiDAR submap. We conducted extensive experiments on the Mulran Radar Dataset, Oxford Radar RobotCar Dataset, and our dataset to demonstrate the feasibility and effectiveness of our proposed approach.
Bleeding Disorder Referrals to Hematology Clinic: A single institution experience
Zuri Hudson
Oluwaseun Olaiya

Zuri Hudson

and 3 more

June 10, 2023
Introduction The evaluation for an inherited bleeding disorder can be challenging. Our hematology clinic receives hundreds of referrals annually for bleeding disorder evaluation due to bleeding symptoms and secondary to preoperative laboratory testing. Aim To characterize hematology referrals for bleeding disorder evaluation at our institution. To describe the diagnostic outcomes, estimate the proportion diagnosed, and identify referral factors that are associated with being diagnosed with a bleeding disorder. Methods This is a single center, retrospective chart review. Patients referred and or seen for a bleeding disorder evaluation from 07/1/2018 until 06/30/19 were included. AIC was applied to logistic regression to identify factors associated with diagnosis of bleeding disorder. Results Of the 373 subjects included, mean (SD) age was 8.3 (5.4) years, 210 (56.3%) female, 256 (69%) white and 69 (18%) black; 40 (11%) were diagnosed with a bleeding disorder and 255 ruled out. Of our referred sample, 6% (21/373) were diagnosed with von Willebrand disease, 4% (14/373) with a platelet function disorder, and 1% (4/373) with a coagulation factor deficiency. Forty percent of referrals were for preoperative clearance, 36% for family history, and 57% for symptoms. The odds of a bleeding disorder diagnosis decreased by 8% for every year increase in age and were 3 times higher among patients having abnormal coagulation labs at the time of referral as compared to their counterparts. Conclusion This study highlights predictive variables for the presence of an inherited bleeding disorder. These results may contribute to future large-scale studies.
Use of Apixaban in Children Awaiting Heart Transplantation
Victor Benvenuto
Christina Hartje-Dunn

Victor Benvenuto

and 6 more

June 10, 2023
Objective The use of apixaban in the pediatric cardiac population is expanding. We describe our apixaban dosing and monitoring strategy in children and young adults awaiting heart transplantation, along with outcomes related to bleeding and thrombosis during wait-list and early post-transplant periods. Methods This study is a retrospective, single center analysis of all patients receiving apixaban while awaiting cardiac transplantation. Weight-based dosing was monitored with peak drug-specific anti-Xa chromogenic analysis. Significant post-operative bleeding defined by chest tube output or need for surgical intervention. Results From September 2020 through December 2022, 19 patients, median age 13.5 years (6.1, 15.8 years), weighing 48.9 kg (15.4, 67.6) received apixaban while awaiting transplant. Indication for apixaban was prophylaxis (n=18, 3 with ventricular assist devices) and treatment of thrombus (n=1). There were no clinically relevant non-major or major bleeding, nor thrombotic events while awaiting transplant. The median time from last apixaban dose to arrival in the operating room was 23.2 hours (15.6-33.8), with median random apixaban level of 37 ng/ml (28.3, 59), 6.3 hours (4.8, 8.4) prior to arrival in the operating room. 32% of patients had significant post-operative bleeding based on chest tube output post-transplant or need for intervention. No patients meeting criteria for significant postoperative bleeding were thought to be attributable to apixaban. Conclusion Careful use of apixaban can be safe and effective while awaiting heart transplant. There was no appreciable increase in perioperative bleeding. The use of apixaban is promising in providing safe, predictable and efficacious anticoagulation while avoiding additional patient stressors.
Long-term effects of surface-water temperature increase on plankton communities in ar...
Kingsly C. Beng
Slawek Cerbin

Kingsly C. Beng

and 3 more

June 10, 2023
Increased surface-water temperatures are predicted to drive dramatic changes to planktonic communities, with consequences for freshwater biodiversity and ecosystem functioning. A large number of short-term mesocosm studies reported temperature-driven changes in plankton, but comparatively few studies captured long-term changes. We used water eDNA metabarcoding to examine communities of phytoplankton, zooplankton, and planktonic protists and fungi in 10 natural lakes in central Poland, five of which have received hot-water discharge from power stations for the past 60 years. eDNA samples were collected in the winter, spring, summer, and autumn of 2020. Heated lakes were 2˚C warmer (annual mean) and had higher concentrations of total phosphorus (TP) and soluble reactive phosphorus (SRP). Based on relative abundance of amplicon sequence variants (ASVs), green algae (Chlorophyta) abundance was up to 15% higher in heated lakes, while that of golden algae (Chrysophyceae) was up to 7% higher in control lakes. ASV richness varied seasonally but was on average two-fold greater in heated lakes, and was consistently higher for phytoplankton, protists, and fungi. Considering the total community, heated and control lakes had distinct plankton assemblages, and there was less temporal variation in heated lakes. Warming was positively related to plankton relative abundance and richness. Our results suggest that increased temperature in heated lakes caused considerable shifts in plankton composition, where groups with preference for cooler temperatures were replaced by those with a preference for warmer conditions.
The Effect of Self and Cross Pollination Using Honey Bee and Development in Complete...
Mahbod Moayeri

Mahbod Moayeri

June 10, 2023
Pollination was a process of transferring pollen grains from the male anther of a flower to the female stigma for plant reproduction and was an important mechanism in the life cycle of plants. This experiment involved a life cycle of Brassica rapa which was a member of the mustard or cabbage family. Brassica rapa was exceptional in its rapid development from seed to seed within four weeks. Morphological aspects and characterization of pollination was an important topic for developing fruit and seed production in the life cycle of the plant. The objective of this experiment was to assess the effects of cross-pollination on fruit and seed production of Brassica rapa. The major methods for this experiment required to use two Styrofoam quads as a control and a cross-pollinated watered the two seeds in each cell for three days of experiment and used honey bee for cross-pollination. The rate of developed fruit and the mean number of seeds were higher on cross-pollination than on self-pollination. Conversely, the average of undeveloped fruit in cross-pollination was 9.677 and the average of undeveloped fruit in self-pollination was 13.937. This result indicates that the rate of undeveloped fruit was higher on self-pollination than on cross-pollination. This study concluded that regulated hydration of pollen for pollination and balanced hydration and dehydration cycle to pollen grains are the key result of this experiment.
Nonlinear dynamic deformation of a rod in the presence of nonstationary extreme exter...
Yuri Chirkunov
M. Yu. Chirkunov

Yuri Chirkunov

and 1 more

June 10, 2023
In this paper, we study a nonlinear model that describes the longitudinal deformation of an elastic rod with a power-law nonlinearity in the presence of non-stationary extreme external action that is very strong at the initial time. For this model, all invariant submodels given by invariant solutions of the equation of this model are obtained. These submodels have not been previously noted in the literature. Invariant solutions of equation which describes this model are found either in explicit form, or their search is reduced to solving systems of differential equations of the first order. For the submodels given by the explicitly found solutions, we found out whether or not the destruction of the rod over time will occur within this submodel. For specific parameter values included in these solutions the distribution longitudinal displacement in the rod are shown in the graphs. In some cases, it was possible to find the place and time of the destruction of the rod. For other submodels, we study physically meaningful boundary value problems that describe non-linear longitudinal deformations of an elastic rod, for which at the initial moment of time at a fixed point either a longitudinal displacement and its gradient, or a longitudinal displacement and a rate of its change are given. The existence and uniqueness of solutions to these boundary value problems are established. under some additional conditions. This allows us to solve these problems numerically correctly.Boundary value problems for some specific values of the parameters included in them are solved numerically. The results of solving these boundary value problems are shown in the graphs.
Hei-Xiao-Yao-San improves amyloid plague in Alzheimer’s disease mice by regulating th...
Hongyan Wu
Wang Yale

Hongyan Wu

and 5 more

June 10, 2023
Amyloid plaques and neurofibrillaric tangles are pathological characteristics of Alzheimer’s disease (AD). Endoplasmic reticulum stress (ERS) is involved in the neuron loss of AD. The aim of this study is to improve the amyloid plaques in an AD mouse model using Hei-Xiao-Yao-San (HXYS), a Chinese traditional medical formula. A mouse AD model was established. AD mice (APP/PS1 mice) were treated with HXYS or saline. Cognitive function in mice was evaluated by using the Morris water maze. The histology of the hippocampus was evaluated by hematoxylin eosin (H&E) staining. The ultra-structure of the hippocampal tissue was observed under a transmission electron microscope.Treatment with HXYS significantly enhanced cognitive function in AD mice. Neural loss and synaptic plasticity of the hippocampus were significantly improved. The amounts of APP (amyloid-beta precursor protein) and ERS associated proteins (CHOP and PERK) in the hippocampus were significantly reduced in AD mice. HXYS may enhance cognitive function in AD mice by regulating ERS.
A Ripple in Time: Timing is Everything
Tarek Zghaib
David Lin

Tarek Zghaib

and 1 more

June 10, 2023
Editorial
A typical, relapsing case of Brugada Syndrome
Erick Alexanderson-Rosas
Mariell Alejandra Rodríguez-Salazar

Erick Alexanderson-Rosas

and 8 more

June 10, 2023
Introduction: Brugada syndrome (BrS) is a primary electrical disorder that has been a subject of study because of its association with cases of sudden cardiac death (SCD). Methods: This article presents a case of a 32-year-old man with no prior cardiovascular disease history who experienced syncope and exhibited electrocardiographic changes. Results: An ajmaline test was performed, which revealed the presence of type II and I Brugada patterns. A literature review was conducted, covering the definition, classification, pathophysiology, genetics, prognosis, and treatment of this condition. Conclusion: Identifying electrocardiographic patterns and taking a correct clinical history can guide a timely therapeutic approach.
The Ins and Outs of Solving Quadratic Equations with Floating-Point Arithmetic
Frédéric Goualard

Frédéric Goualard

June 09, 2023
Solving quadratic equations with radicals on a computer with floating-point arithmetic requires great care to handle correctly all possible parameters. Literature on the subject glosses over the details, often considered as important but tedious to present. As a consequence, most implementations are flawed in one way or another. After having reviewed both the literature and the actual implementations in several programming languages and applications, we present an algorithm inspired from an exposition by Pat Sterbenz from 1974, adapted to take advantage of more recent researches in the field, which leads to a robust quadratic equation solver.
Predicting Software Vulnerability Based on Software Metrics: A Deep Learning Approach
Francis Kwadzo Agbenyegah
Jinfu Chen

Francis Kwadzo Agbenyegah

and 4 more

June 09, 2023
Failures in computer systems are caused by flaws in the design of the computer systems, bugs and vulnerabilities. Vulnerabilities in software is inevitable, hence there should be a mechanism in place to detect, locate and remove these vulnerabilities in computer systems before being deployed. Mostly used in the vulnerability prediction model (VPM) are the text-mining and software metrics, which results into low accuracy and few recall. This paper investigates the impact of the non-used metrics and the mostly used metrics in predicting the availability of bugs in software code. The deep learning algorithm used in the design of the VPM includes convolutional neural network (CNN), multilayer perceptron (MLP), long term short memory (LSTM) and combine the MLP and CNN. The experimental results show that, known software metrics (TSM) and uninvestigated software metrics or new software metrics (NIM) are not ideal for vulnerability prediction since they all shows poor performance.
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