Background Despite its clinical benefits, patient compliance to remote monitoring (RM) of cardiac implantable electronic devices (CIEDs) varies and remains under-studied in diverse populations. Objective We sought to evaluate RM compliance, clinical outcomes, and identify demographic and socioeconomic factors affecting RM in a diverse urban population in New York. Methods This retrospective cohort study included patients enrolled in CIED RM at Montefiore Medical Center between December 2017 and May 2022. RM compliance was defined as the percentage of days compliant to RM transmission divided by the total prescribed days of RM. Patients were censored when they were lost to follow-up or at the time of death. The cohorts were categorized into low (≤30%), intermediate (31-69%), and high (≥70%) RM compliance groups. Statistical analyses were conducted accordingly. Results Among 853 patients, median RM compliance was 55%. Age inversely affected compliance (p<0.001), and high compliance was associated with guideline-directed medical therapy (GDMT) usage and implantable cardioverter defibrillator (ICD)/ cardiac resynchronization defibrillator (CRTD) devices. The low-compliance group had a higher mortality rate and fewer regular clinic visits (p<0.001) than high-compliance group. Socioeconomic factors did not significantly impact compliance, while Asians showed higher compliance compared with Whites (OR 3.67; 95% CI 1.08-12.43; p=0.04). Technical issues were the main reason for non-compliance. Conclusion We observed suboptimal compliance to RM, which occurred most frequently in older patients. Clinic visit compliance, optimal medical therapy, and lower mortality were associated with higher compliance, whereas insufficient understanding of RM usage was the chief barrier to compliance.

In the actual traffic environment, there usually exists more than one vehicle in the radiation area of the road-side units (RSU) at the same time, and the front vehicle will affect the ray paths between the RSU and the on-board units (OBU) of the back vehicle. Here we studied the front vehicle’s impact on the ETC system’s path propagation loss by ray tracing technology and Uniform Theory of Diffraction (UTD). Firstly, we simplified the vehicle body structure into two equivalent geometric models. We analyzed propagation loss models under line-of-sight (LOS) and non-line-of-sight (NLOS) conditions based on the distance change among RSU, OBU, and the front vehicle. Finally, a set of ETC comprehensive testing equipment was developed to measure the transmission loss of toll stations. Both simulation and experiment results indicate that the propagation loss models proposed in this letter are valid.

Background: Small airway dysfunction (SAD) is a common problem in childhood asthma patients despite of asthma control therapy. The effectiveness and mechanism of allergen specific immunotherapy(AIT) on small airway dysfunction in children with asthma remains unclear. The purpose of this study is to investigate the the effectiveness of AIT on SAD and mechanism underline with special focus on basophil. Methods: 65 mild to moderate asthma children under regular ICS treatment for more than one year, but their FEF 75 remained below the 65% of predicted and with positive results of serum Der p or der f were enrolled. Asthma children underwent house dust mite (HDM) subcutaneous immunotherapy (SCIT) treatment for 6 months. Asthma control and lung function were evaluated every three months during HDM SCIT treatment. Basophil activation test was carried out before and after HDM SCIT treatment. RNA-sequence were performed in isolated basophil from peripheral blood after 6 mionths of HDM SCIT treatment followed by GO term and KEGG pathway enrichment analysis between patients with or without HDM SCIT treatment. Results: The patients’ childhood asthma control test(C-ACT) scores have risen above the baseline value after 3 and 6 months’ treatment. The percentage of patients with complete asthma control was also increased from 52.4% to 75.8% (after 3 months of AIT treatment) and 73.7% (after 6 months of AIT treatment). Meanwhile, the percentage of uncontrolled asthmatic patients (C-ACT score＜20) dropped from 9.52% to 3 % and 0% after 3 and 6 months’ treatment of AIT, respectively. AIT treatment also improved lung function parameters such as FEV 1/FVC, FEF 75, FEF 50 and MMEF after the first 3 months’ therapy (p＜0.05). FEF 75 values showed a highly significant, gradual and persistent increase (from 49.55 ± 1.27% at baseline to 65.56 ± 2.89 % and 71.89 ± 2.64 % after 3 months’ and 6months’ therapy, respectively). 24 of 32 patients were out of SAD after 6 months’ treatment. BAT results revealed that AIT treatment significantly reduced basophil activity to HDM in vitro challenge from baseline. GO term and KEGG pathway enrichment analysis of basophil revealed that downregulated genes mainly involved in immune cell activation, antigen presenting procedure and Th cell differentiation. Conclusions: Our current study demonstrated that HDM AIT not only improved asthma symptom and clinic parameters, but also increased lung fuction parameters, especially improved SAD measured by FEF 75, FEF 50 and MMEF. We also demonstrated that HDM AIT reduced basophil activity. RNA-sequence of basophil revealed the inhibiton of phagocytosis and phagosome pathway which is required for the APC function of basophils and therefore may affect the polarization of Th2 cell differentiation. However, further in vivo and animal study are required to confirm those results.

With the widespread popularity of electric vehicles (EVs), the problem of energy structure could be alleviated, but it also increases the pressure on the power supply side, so the charging load prediction has a wide range of application scenarios and a huge commercial value. The most of existing EV charging load forecasting methods are modeled from the perspective of charging stations, ignoring the travel habits and charging needs from the perspective of users. In this paper, a temporal spatial neural network model based on graph attention and Autoformer is proposed to predict EV charging load, and a spatiotemporal graph data set based on user travel trajectory is constructed. The experimental results show that the proposed method can fully tap the distribution of user clusters in time and geographical space, to effectively improve the accuracy of charging load prediction.

Inflammatory myopathy in the proximal lower limb: Rare presentation of lymphomaOmid pourbagherian1, Mehrzad Hajialilo2, Amir Vahedi3, Ali Esmaeili4, Mehdi Jafarpour21 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran2Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran3Department of Pathology, Tabriz University of Medical Sciences, Tabriz, Iran.4 Department of Pathology, Valiasr Hospital, Tabriz, Iran.Drafting of the manuscript: Omid pourbagherian, Mehrzad Hajialilo, Mehdi JafarpourData Collection: Mehrzad Hajialilo, Amir Vahedi, Ali Esmaeili, Mehdi JafarpourCritical revision of the manuscript for important intellectual content: Mehdi Jafarpour, Omid pourbagherianCase supervision: Mehdi Jafarpour, Mehrzad HajialiloCorresponding author: Mehdi JafarpourAddress: Connective Tissue Diseases Research Center, Flat 1, Imam Reza Hospital, Tabriz, IranPhone number: 0098 41 33332704Fax: 0098 41 35413520Mobile: 09148651269Keywords: Inflammatory myopathy, lymphoma, inflammation, cancerWritten informed permission from the patient to publish the facts and photographs was granted.

Enteroliths, faecaliths, ingested foreign bodies, and bezoars are examples of focal intraluminal obstructions that can lodge in inaccessible parts of the gastrointestinal tract. Pneumatic lithotripsy, as described in the accompanying article, is an option to manage obstructions caused by mineralized concretions. However, pneumatic lithotripsy may not be safe or effective for all intraluminal obstructions. Awareness of other strategies or secondary abdominal approaches to address these challenging presentations can help improve the surgeon's ability to problem solve in these situations and achieve successful resolution of the obstruction.

Increasing evidence indicates that tree growth processes, including reproduction, can be either sink- or source-limited, or simultaneously co-limited by sink and source, depending on the interplay between internal and environmental factors. We tested the hypothesis that the relative strengths of photosynthate supply and demand by stem growth and reproduction create variable competition for substrate that is imprinted in tree-ring isotopes of Pinus pinea, a gymnosperm with large costs of reproduction, under warming-induced drought. Over a 60-yr period, we also identified reproductive phases where weather drivers of cone yield have varied. We found that these drivers gradually shifted from winter-spring conditions three years before seed rain (cone setting) to a combination of three- and one-year lagged effects (kernel filling). Together with positive associations between Δ 13C of the year of kernel filling and cone yield arising at the turn of this century, our results pinpoint increasing dependence of reproduction on fresh assimilates. This indicates sink and source co-limitation superseding the sink-limited functioning of reproduction dynamics dominant before 2000. Under climate warming, it could be expected that drier conditions reinforce the role of source limitation on reproduction and, hence, on regeneration, forest structure, and economic profit of the nutlike seeds of the species.

Introduction: Thalassemia and myeloproliferative neoplasias (MPNs) are two separate disease entities. Thal. is an inhereted congenital hemolytic anemia caused by abnormal globin gene synthesis. MPNs are acquired clonal hematopoietic stem cell diseases characterized by the proliferation of different hematopoietic stem cells. The coexistence of thal. and MPNS are rarely reported. Methods: We performed a single-center retrospective analysis of all cases known to have thal. including patients developing persistent high levels of hemoglobin or platelet. The diagnosis of MPN was confirmed by genetic testing from peripheral blood, bone marrow biopsy and blood film analysis. Results: In this case series, we report six cases with coexistence of alpha(α), beta(β) and delta-beta (δβ) thal. with different types of MPNs including essential thrombocytosis ( ET ) and polycythemia vera. Majority of these patients had positive Janus kinase (jak 2) mutation, except for one patient with diagnosis of ET who had calreticulin (CALR) gene mutation. Most of the patient in our case series are younger than average age for the diagnosis of MPNs.

The nanofibers formed by electrospinning technology have the advantages of orderly release mechanism and controllable dissolution rate. However, the use of toxic and harmful organic solvents is widespread in the field of electrospinning, which not only poses a potential hazard to human health and the natural environment, but also increases the manufacturing cost and difficulty of electrospinning. Therefore, the purpose of this paper is to introduce the application of green electrospinning technology in food engineering. Water solvent electrospinning is mainly introduced, and some water-soluble biopolymer and synthetic polymers are reviewed. Their latest applications and development limitations in the field of food engineering are introduced and discussed. The water solvent electrospinning introduced here transforms a process that has adverse effects on the environment and health into a scalable and ecologically conscious “green” electrospinning, which is both important and timely and will bring exemplary changes to the field of food engineering.

Objective: This study aimed to test the inter-rater reliability, construct validity, and feasibility of the modified “Which Health Approaches and Treatments Are You Using?” (WHAT) questionnaires in pediatric oncology. Methods: A sample of parent-child dyads was invited to complete self- and proxy-report modified WHAT, Pediatric Quality of Life Inventory, demographics, a diary of child recent use of CHA, and a questionnaire assessing aspects of feasibility. Parents were also asked to complete a satisfaction of children’s use of CHA survey. Results: Twenty-four dyads completed the study. The mean weighted kappa showed strong inter-rater reliability (k=0.77, SE=0.056); and strong agreements between the modified WHAT and the diary (self-report [k=0.806, SE=0.046], proxy-report [k=0.894, SE=0.057]). Significant relationships were found only between recent and non-recent CHA users in relation to easy access to CHA (self-report [p=0.02], proxy-report [p<0.001]). The mean scores of the feasibility scale (out of 7.0) for the self- and proxy-report were 5.64 (SD=0.23) and 5.81 (SD=0.22), respectively, indicating the feasibility of the modified WHAT. Conclusions: The findings of this study provide initial evidence of the reliability and validity of the modified WHAT questionnaires and their feasibility in assessing child CHA use and initiating clinical discussions about CHA with children and their parents. Further research is needed to test the theoretical relationships and to further explore the validity and reliability of the modified WHAT.

In the Alpine Meadow ecosystems of eastern Qinghai-Tibet Plateau, the interrelations among the species diversity of different vegetation communities, productivity, community structure as well as soil nutrients were thoroughly researched through running biodiversity manipulation experiment to explore the species survey consequences of 3 and 10 years of plant functional groups (Gramineae, Cyperaceae, Legumes, and other Forbs) removal at Haibei station. The results demonstrated that the interannual variation of the remaining species richness, above-ground and below-ground biomass of the community gradually presented a tendency to decrease as the removal time increased, and there was a positive relationship between species richness and productivity, and the correlation became increasingly significant. The removal behavior reduced the number of Gramineae within the remaining community. The content of soil total nitrogen, phosphorus, organic matter and moisture content of Legumes loss treatment increased significantly. The treatment that removal Forb had the lowest negative cohesion values, revealing that it is difficulty for this community to recover to the previous equilibrium state in a short time. In our study, all affects of species removal on ecosystem may be related to variance in the structure and composition of species in community. Meanwhile, changes in the number of Gramineae indicated that Gramineae are more sensitive and less resistant to removal behavior. Furthermore, the specific performance of Legumes removal indirectly indicates that the loss of diverse plant function groups prompted distinct replies to the starvation and compensation effects. In a nutshell, species loss at the community level leads to shifts in the niche of each species, inducing a redistribution of community resources and leading to significant changes in community structure.

Background. Race and ethnic disparities in cancer incidence rates and the prevalence of cytomegalovirus (CMV) are known to exist in the United States (U.S.) but also across broad geographic expanses. The prevalence of CMV seems to inversely contrast rates of tumor incidence both in ethnic groups and globally. Is there a biological link between cancer and CMV infection? Methods. Global cancer data were retrieved from the World Health Organization (WHO) database. Incidence of cancer and CMV seroprevalence (73 countries) were subjected to Spearman’s correlation test. The Bayesian framework was adopted for CMV seropositivity variables. Cancer incidence and CMV pervasiveness in the U.S. were extracted from publications based on the Surveillance, Epidemiology, and End Results (SEER) registries and the National Health and Nutrition Examination Surveys (NHANES), 1988-2004. Results. An inversely directed coupling between cancer and CMV seropositivity across diverse ecologies and cultural domains suggest a global oncoprotective effect of the CMV (Spearman’s ρ = -0.732; p<0.001). Rates of all cancers combined and CMV seropositivity show an opposite association ( p<0.001) among the races and foremost U.S. ethnic groups. Conclusion. The racial/ethnic incidence of cancers and CMV seropositivity are inversely proportional both in the U.S. and globally. This would support a view that CMV is a potential driver against tumorigenesis. An absence of CMV infection abrogates protection against malignant clones afforded by the virus to an infected host. Abating CMV seroprevalence may relate causally to the buildup of malignancies in U.S. and the West world countries with thriving hygiene and healthcare systems.

The unsteady two immiscible MHD free convective flow of Casson liquid through a vertical channel, with a porous medium, is studied numerically in this investigation. Using a double perturbation approach, the governing flow equations are reduced to a system of connected partial differ ential equations, which are then solved using the 4th-order numerical  Runge-Kutta method coupled with the shooting approach in Mathematica. The velocity and thermal slip conditions have been accommodated in this model. The interaction of permeability of the porous medium, energy dissipation, Joule heating, and thermal radiation are taken into consideration. The computational upshot is also de- scribed explicitly to examine the consequences of pertinent parameters. The computational results are analyzed to investigate the effects associated with pertinent parameters which include the Hartmann number of two regions, Darcy number, a ratio of Porous medium permeability, Grashof number, Radiation parameter, heat source, and Prandtl num ber. The characteristics of the essential regulating parameters on flow frameworks of velocity, temperature, Entropy generation, Bejan number, and heat transfer rate are analyzed correctly via plots, and skin friction and flow rate are given in tabular form. As the radiation parameter raises both the velocity and temperature of the Casson fluid decreases.  The rate of entropy generation falls with a rise in the magnetic field. The Bejan number escalates as it moves forward from the lower wall to the channel’s center. In the later part of the channel, the Bejan number starts to drasti- cally fall and reaches a minimum at the upper wall.

This study develops a methodology to create detailed visual Digital Twins of large-scale structures with their realistic damages detected from visual inspection or nondestructive testing (NDT). The methodology is demonstrated with a transition piece of an offshore wind turbine and a composite rotor blade, with surface paint damage and subsurface delamination damage, respectively. Artificial Intelligence and color threshold segmentation are used to classify and localize damages from optical images taken by drones. These damages are digitalized and mapped to a three-dimensional geometry reconstruction of the large-scale structure or a CAD model of the structure. To map the images from 2D to 3D, metadata information is combined with the geo placement of the large-scale structure’s 3D model. After mapping the damage, the Digital Twin gives an accurate representation of the structure. The location, shape, and size of the damage are visible on the Digital Twin. The demonstrated methodology can be applied to industrial sectors such as wind energy, the oil and gas industry, marine and aerospace to facilitate asset management.

COVID-19 has altered the existing organizational dynamics. Almost every sector within a few weeks introduced virtual working, where it was feasible. Even sectors already practicing work from home with select groups made it mandatory for all. The study covers the impact of COVID--19 induced lockdowns on employee productivity in select sectors of the service industry. Respondents from the BFSI, IT, and Education sectors were drawn to record their responses. The results have demonstrated a significant impact of remote working on employee productivity across sectors and constructs. However, the degree of impact varied from sector to sector. This paper also highlights issues in digital adaptation. This study may be considered one of the initial attempts to investigate the impact of the universalization of remote on employee productivity in dominant service verticals. The findings suggest employees have accepted remote working as a feasible alternative to physical workplaces, since they find remote working provides the flexibility to balance work and life. The HR managers can apply the findings from this study to apply remote working as a strategic tool to attract and retain talent. This paper also contributes to the scant literature on employee productivity during an extraordinary global crisis.

Purpose Phase 1 study assessing the safety and toxicity of cabozantinib in combination with topotecan and cyclophosphamide for relapsed osteosarcoma and Ewing sarcoma. Methods Oral cabozantinib (25mg/m 2) was administered daily for 21 (dose level 1) or 14 (dose level -1B) days. Topotecan (0.75mg/m 2) and cyclophosphamide (250mg/m 2) were administered IV on days 1-5. A modified 3+3 design based upon first cycle dose-limiting toxicities (DLT) was used for dose escalation. Results Twelve patients with a median age of 15 (12.9-33.2) years were enrolled (7 with Ewing sarcoma; 5 with osteosarcoma); all were evaluable for toxicity. At dose level 1, three of six patients developed first cycle DLT: grade 3 epistaxis; grade 3 transaminitis; prolonged grade 2 thrombocytopenia. Six patients were enrolled on dose level -1B (interrupted cabozantinib, given days 8-21), with one first cycle DLT (grade 3 pneumothorax) observed. Of the 10 response evaluable patients, one had partial response (Ewing sarcoma), seven had stable disease, and two had progressive disease. Conclusions The recommended phase 2 doses and schedules for this combination are topotecan 0.75mg/m 2 IV days 1-5, cyclophosphamide 250mg/m 2 IV days 1-5, and cabozantinib 25mg/m 2 days 8-21. Non-concomitant administration of cabozantinib with cytotoxic therapy in this population has acceptable toxicity while allowing for potential disease control.

HPV16 is the primary etiological factor for cervical cancer, with typical mutations that have varied geographical distributions and carcinogenic potency. Detecting HPV16 mutations, therefore, has consequences for cervical cancer screening, clinical diagnosis, and therapy. In Vietnamese women with cervical cancer, HPV16-positive samples were analyzed for nucleotide sequence alterations using Sanger sequencing of the E6, E7, and L1 genes. The HPV16 variants were identified using ATGC 7.2, and the phylogenetic tree was constructed using MEGA 11.0.10. Among 180 patients infected with HPV, 76.1% revealed single infections, and 24.9% showed multiple infections. The most common HPV genotypes were HPV16 (63.9%), HPV18 (26.7%), and other HPV (6.9%). HPV16 alterations were found in all E6, E7, and L1 genes, with 15 missense and 18 synonymous mutations. Missense mutations include: R10G, Q14H, D25E, H78Y, L83V (E6); M29V, R35K, L78R, L95P (E7); H73Y, T176N, N178T, T317P, T386S, L472F/I (L1). HPV16 sublineages include A1 (17.2%), A2 (0.9%), A3 (56.0%), A4 (19.0%), D1 (4.3%), and D3 (2.6%). Our findings showed that HPV16 is prevalent in cervical cancer in Vietnam, with the European lineage distribution predominating. Although HPV16 is responsible for a significant number of cervical cancers, the L83V mutation rate is only observed in 6.9% of samples.

Non-coding RNA species play important roles in biological mechanisms that regulate glioma initiation and progression. Recently, evidence suggests that ZNFX1 antisense RNA 1 ( ZFAS1) has the ability to act as an oncogene or tumour suppressor, and so plays critical regulatory functions in the development and progression of many types of cancers such as lung, renal and hepatocarcinoma. The roles of ZFAS1 in glioma cancer are still unclear, and there are numerous potential pathways to explore. The current work provides a scoping review of studies on ZFAS1’s functions and underlying molecular mechanisms in the initiation and progression of glioma, as well as a possible field of research to be examined further. A literature search was carried out using Scopus, PubMed, and Web of Science (WoS) using a specified search string, and the data gathered was discussed and reported. This scoping review comprised five original research papers that study ZFAS1 and its roles in gliomagenesis. ZFAS1 was found to be highly upregulated in glioma. Tumour-node-metastasis (TNM) stage, lymph node metastases, and overall survival were revealed to be significantly associated with ZFAS1 status and regulated via several pathways and interactions, such as miRNA signalling, Epithelial-to-Mesenchymal Transition (EMT) and Notch signalling pathway. Furthermore, ZFAS1 knockdown decreased cell proliferation, migration, and invasion while promoting cell death, implying that ZFAS1 is involved in the glioma cancer progression. The evaluation of their diagnostic importance and therapeutic potential may aid in the development of novel therapies for glioma cancer.

Background Doege-Potter syndrome is defined as paraneoplastic hypoinsulinemic hypoglycemia associated with a benign or malignant solitary fibrous tumor frequently located in pleural, but also extrapleural sites. Hypoglycemia can be attributed to paraneoplastic secretion of ‘Big-IGF-II’, a precursor of Insulin-like growth factor-II. This prohormone aberrantly binds to and activates insulin receptors, with consecutive initiation of common insulin actions such as inhibition of gluconeogenesis, activation of glycolysis and stimulation of cellular glucose uptake culminating in recurrent tumor-induced hypoglycemic episodes. Complete tumor resection or debulking surgery is considered the most promising treatment for DPS. Case Here we report a rare case of a recurrent Doege-Poter Syndrome with atypical gelatinous tumor lesions of the lung, pleura and pericardial fat tissue in an 87-year-old woman. Although previously described as ineffective, we demonstrate that adjuvant treatment with Octreotide in conjunction with intravenous glucose helps to maintain tolerable blood glucose levels before tumor resection. The somatostatin-analogue Lanreotide was successfully used after tumor debulking surgery to maintain adequate blood glucose control. Conclusion We conclude that somatostatin-analogues bear the potential of being effective in conjunction with limited surgical approaches for the treatment of hypoglycemia in recurrent or non-totally resectable SFT entities underlying DPS.

Background Immunogenic cell death (ICD) is a type of regulated cell death that is sufficient to trigger an adaptive im-mune response. ICD has been demonstrated to activate the tumor-specific immune response, and inducing ICD would be a potential treatment strategy. Aims The biomarkers of ICD and their relationships with tumor microenvironment, clinical feathers, and immunotherapy response are not fully understood in the context of the clinic. We therefore conducted pan-cancer analyses of ICD gene signatures in 33 cancer types from The Cancer Genome Atlas database. Methods and results We identified key genes that had strong relationships with survival and tumor microenvironment. Besides, we predicted therapeutic agents targeting ICD genes and explored the potential mechanisms of gemcitabine to induce ICD. Moreover, we determined an ICD score based on the ICD genes and found that the ICD score was associated with patient prognosis, clinical features, tumor microenvironment, radiotherapy access, and immunotherapy response. The high ICD score was associated with the immune-hot phenotype, and the low ICD score was associated with the immune-cold phenotype. Conclusion We discovered the potential of the ICD gene signatures as ICD biomarkers in pan-cancer, which may provide new insights into immuno-phenotypic assessment and cancer therapeutic strategies, allowing more patients to benefit from immunotherapy.

As the landscape of information systems rapidly evolves, Service Oriented Architecture (SOA) stands out as a potent solution, offering organizations the agility needed to keep pace. By encapsulating application functionality into interoperable services, SOA enables the strategic orchestration of highly adaptive business processes. This shift from traditional web services to SOA is gaining momentum across various sectors. Standardized information technologies facilitate this transition, transforming resources into reusable services and fostering inherently adaptable environments. This research article presents a detailed exploration of SOA and a practical case study from a technology firm based in Pereira, Colombia. This case study highlights both the operational enhancements brought about by SOA and the unexpected positive impacts on employee satisfaction after its implementation. Combining theoretical analysis with a real-world example, this research emphasizes SOA’s potential in reshaping the future of web services. It enriches the existing body of knowledge with empirical evidence and opens up new avenues for future research on the wider impacts of SOA in different organizational settings.

The proliferation of chatbots in recent months has raised concerns about the potential for bias in these conversations. This is especially true when it comes to public health topics, where accurate and unbiased information is essential. Herein, the focus is on the issue of bias in chatbot conversations using oral contraceptive pills (OCPs) as an example. By raising awareness about this issue and emphasizing the need for critical evaluation, we can empower individuals to navigate the digital landscape with confidence and make informed decisions about their health.

DnaJ proteins, constituents of the heat shock protein system, protect cells from environmental stresses as HSP-70 molecular co-chaperones. Here, we demonstrated that a pathogen-induced AdDjSKI, a serine-rich DnaJ III protein plays an important role in the stability of photosystem II in response to heat stress. Under high temperatures, transplastomic tomato plants expressing the AdDjSKI gene showed increased levels of total soluble proteins, better growth, higher chlorophyll content, lower malondialdehyde (MDA) accumulation and reduced Photosystem (PS) II photoinhibition compared to the wildtype (WT) control plants. Interestingly, the transplastomic plants showed higher D1 protein levels under high temperatures compared to the WT plants suggesting that the overexpression of AdDjSKI in the plastids played a key role in the protection of PSII probably because of its chaperone activity. Additionally, the transplastomic plants showed relatively lower accumulation of superoxide radical (O 2 •-) and H2O2 levels in comparison with the WT plants possibly due to higher ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities, which was due to an increase in related gene expression under heat stress. This study also reveals the chloroplast expression of AdDjSKI in tomato play a critical role in fruit yield, primarily through a combination of delayed senescence and enhanced carbon assimilation.

The conversion of triglycerides in edible oils into diacylglycerols (DAGs) is of great significance for a healthy lifestyle. Camellia Seed Oil (C-oil), which is rich in oleic acid and linoleic acid, is an excellent raw material for the production of DAG. In this study, the results showed that under the optimum conditions, the hydrolysis rate was 87.14 %, and the content of DAG was 62.49 % after the hydrolysis product was esterified with glycerol. The C-oil, C-FFA, and Camellia Seed Oil diacylglycerol (C-DAG) were characterized using GC, TG, DSC, and GC-IMS. The results showed that the enzymatic coupling method did not affect the structural composition of the substances themselves, but it did affect the crystallization and melting properties of the oils and the taste of C-DAG was more delicate than that of C-oil. Finally, the reaction mechanism was analyzed using infrared spectroscopy, which showed that C-oil had been mostly hydrolyzed into free fatty acids, and C-DAG exhibited ester C-O stretching vibrations at 1280-1030 cm-1, indicated that the esterification reaction between C-FFA and glycerol had successfully occurred under the catalysis of the enzyme.