The prevalence of hazardous drinking among females has increased more in the past decades as compared to among males. While it is well-known that females suffer more from the negative effects of alcohol use than males, the mechanisms that explain sex differences in hazardous drinking remain poorly understood. The current study aimed to investigate sex differences in the relationship between cue-induced (relief and reward) craving and cue-induced heart rate variability in a sample of hazardous drinkers. One hundred fourteen hazardous drinkers (61 females) underwent an alcohol cue-exposure paradigm consisting of exposure to alcohol-related pictures, personalized script imagery, and alcohol paraphernalia. Relief- and reward cravings were measured before and after exposure using the Desire for Alcohol Questionnaire. Heart Rate Variability was measured continuously during the exposure using an electrocardiograph. While there was a significant increase in both reward and relief craving following the alcohol exposure paradigm, these effects were not moderated by sex. Importantly, however, exposure to alcohol cues was associated with a stronger reduction in Heart Rate Variability among males compared to female hazardous drinkers. Lastly, exposure-related reduction in Heart Rate Variability was significantly stronger in individuals that reported more exposure-induced relief craving, but these effects were not moderated by sex. In summary, our findings suggest that while there were no sex differences in exposure-induced Heart Rate Variability, there was in exposure-induced craving. These findings may pave the way for developing sex-specific interventions specifically targeting Heart Rate Variability, including Heart Rate Variability biofeedback.

Because temperature has pervasive effects on biological rates, climate warming may alter the outcomes of interactions between insect hosts and their parasitoids, which, for many host species, constitute the single largest source of mortality. Many studies report that climate warming is depressing the performance of parasitoids more than that of hosts. We examined this consensus by assessing the thermal ecology of a host-parasitoid interaction in the Rocky Mountains using wild populations of the aspen leaf miner (Phyllocnistis populiella) and a set of eulophid wasps that attack them. Host and wasp development rates were differentially sensitive to temperature. In addition, upper thermal limits in adult wasps were lower than those of host caterpillars, and wasps preferred low temperatures in choice experiments. When coupled to simulations of leaf microclimates in aspen canopies, these observations suggest, contrary to expectations, that climate warming is likely to benefit parasitoids at the expense of hosts.

INTRODUCTIONAbsent pulmonary valve syndrome (APVS) with an intact ventricular septum (IVS) or small ventricular septum is a rare type of APVS associated with high perinatal mortality in fetuses. Although APVS/IVS constitutes only 7.5% to 25% of the APVS cohort, most fetuses (80%) will not survive to birth without inotropic support or heart transplantation due to fetal heart failure, hydrops fetalis, extracardiac and chromosomal abnormities, and pregnancy termination[1-4]. A ductus arteriosus(DA) is usually present in APVS/IVS patients, and a large DA could result in bronchial compression leading to severe respiratory symptoms and signs of congestive heart failure early after birth[5]. With the improvement of postnatal care and intervention, the survival of subjects rises from 20% to 80%[2]. However, long-term surviving analysis remains unknown in patients with APVS/IVS.Symptoms varies according to individual in these patients. Here, we report a rare subtype of APVS/IVS patient with the combination of the absent pulmonary valve leaflets, intact ventricular septum and superior vena cava who develop a surprisingly long-term survival and underwent timely pulmonary valve implantation surgery.

IntroductionSubungual onycholemmal cysts (SOC) also known as subungual epidermoid inclusion cyst is an uncommon nail abnormality which affect the dermis of the nail bed. SOC has variable clinical presentations include onychodystrophy, ridging, clubbing, thickening, pigmentation or even appears normal (1). It can also mimic different nail malignancy such as melanoma, SCC or glomus tumor (2). In this report we document a case of unilateral second right finger nail onychodystrophy in 54-year-old man.

Uterine leiomyomas (fibroids) are common benign tumours affecting women of reproductive age. While most are asymptomatic, a significant proportion experience symptoms affecting their quality of life. Surgery is a definitive treatment for fibroids, where minimally invasive surgical (MIS) techniques have become the preferred approach for preserving fertility. This narrative review provides an overview of the current literature regarding fertility outcomes of different MIS techniques, including conventional laparoscopy, robot-assisted laparoscopy myomectomy (RALM), and mini-laparotomy. The studies reviewed reveal favourable pregnancy outcomes in general which were comparable between laparoscopy, and RALM, single-site and mini-laparotomy. In RALM, higher preterm rates were associated with a higher number of fibroids being removed. The data was inconsistent regarding laparotomy compared to laparoscopy. Further comparative research may be warranted to determine the specific differences regarding fertility outcomes.

Current worldwide estimation of Spent Coffee Grounds (SCGs) is evaluated at a global annual of 23 million tons which constitute actually an abundant Bio-Feedstock that needs to be concerned for a strategy of Bio-Valorization. New technological confronts are progressing to extend the use of SCGs for sustainable green composites like porous bio-adsorbents, modified resins and specifically Phase Change Materials (PCMs) developed for energy storage.

Studies of ectotherm responses to heat extremes often rely on assessing absolute critical limits for heat coma or death (CTmax), however, such single parameter metrics ignores the importance of stress exposure duration. Furthermore, population persistence may be affected at temperatures considerably below CTmax through decreased reproductive output. Here we investigate the relationship between tolerance duration and severity of heat stress across four ecologically relevant life-history traits (productivity, sterility, coma, and mortality) using the global agricultural pest Drosophila suzukii. For the first time, we show that for sublethal reproductive traits, tolerance duration decreases rapidly and exponentially with increasing temperature (R2>0.97), thereby extending the Thermal Death Time framework recently developed for mortality and coma. Using field micro-environmental temperatures, we show how thermal stress can lead to considerable reproductive loss at temperatures with limited heat mortality highlighting the importance of including limits to reproductive performance in ecological studies of heat stress vulnerability.

Objective: This study intended to analyze the prognosis of patients with stage IIIC squamous cervical cancer who underwent the Obstetrics and Gynecology Hospital of Fudan University (FUOG) Treatment, and explored the factors influencing their prognosis. Design: A retrospective study. Setting: A large tertiary hospital specializing in obstetrics and gynecology in China. Population or Sample: This study collected data from 717 patients with stage IIIC squamous cervical cancer who underwent FUOG Treatment in our hospital from January 2016 to December 2020. Methods: Kaplan-Meier method was used to estimate progression-free survival (PFS) and overall survival (OS). Stratified analysis was performed to examine the risk factors. Main Outcome Measures: The main outcomes were 3-year PFS and OS. Results: The 3-year OS was 90.9% for patients with stage IIIC squamous cervical cancer, 91.5% for stage IIIC1 and 83.2% for stage IIIC2, respectively. The 3-year PFS was 84.8%, 85.3% for stage IIIC1 and 78.8% for stage IIIC2, respectively. Undifferentiated squamous carcinoma was an independent prognostic factor for OS (HR: 5.793, p=0.0064) and PFS (HR: 4.663, p=0.0033). Postoperative patients with standard adjuvant therapy had better 3-year OS outcomes than patients with non-standard therapy (88.4% vs 73.4%, p=0.007). Patients with undifferentiated type (OR=8.471), positive parietal infiltration (OR=3.339), or tumor infiltration depth of 1/3-2/3 (OR=5.454) were more likely to have distant recurrence. Conclusions: The prognosis of patients with stage IIIC cervical squamous carcinoma treated with the FUOG Treatment is satisfactory. However, risk factors such as undifferentiated type, positive paracervical infiltration, and non-standard adjuvant therapy can negatively affect prognosis.

For the automated analysis of I/V-characteristics of solar cells and modules, descriptive parameters are essential. In particular with the rise in machine-learning techniques and the related increase data volumes, there is a need for good, general purpose, descriptive parameters. The most commonly used descriptive parameters for I/V are the standard solar cells parameters, consisting of V oc , I sc , V mpp , and I mpp . Also other representations may be considered, such as one diode model parameters corresponding to a particular I/V. However, these representations are very coarse and cannot distinguish or represent many common (non-ideal) features of an I/V (e.g. an S-shape). In this work we propose an extended set of solar cell parameters, which are well defined, and easy to determine. We evaluate the effectiveness of the extended solar cell parameters by reconstructing the I/V from the extracted parameters. This allows one to “measure” information loss. We compare the accuracy of our parameters with other commonly used curve models for I/V, namely the one diode model, and the Karmalkar-Haneefa model. The models are applied to a large set of I/V (about 2.2 million curves), covering a wide range of technologies and conditions. We demonstrate our extended solar cell parameters consistently provide an accurate description of nearly all I/V in these datasets. Furthermore, we present our I/V analysis tool which we use to process these datasets. This tool is fast and capable of extracting the extended solar cell parameters, as well as parameters for the one diode model and the Karmalka-Haneefa model. Finally, we exemplary show how the extended solar cell parameters may be used to detect partial shading in outdoor data, by training a simple random-forest classifier based on extended solar cell parameters.

Rice, one of the most agronomically important crops, has been challenged by high salinity that affects its growth at the seedling stage and reproductive phase. Thus, investigating the intricate molecular mechanisms that regulate its developmental process throughout its life cycle is essential for better stress resilience. In this study, we have investigated the role of rice trithorax group factor ULTRAPETALA ( OsULT1) that orchestrates rice development and stress response. A genome-wide chromatin immunoprecipitation analysis revealed enrichment of OsULT1 to transcription factors and regulators, oxidative stress signaling, ROS scavengers, and K + uptake transporters, during salinity stress. Interestingly, loci associated with root development, plant height, inflorescence development, panicles, spikelet numbers, and seed development also showed OsULT1 occupancy under control and salt stress. OsULT1 transcriptionally regulates these loci during stress response by modulating H3K4me3 and H3K27me3 modifications. OsULT1 overexpression ( OsULT1-3) causes developmental changes in rice with enhanced plant height, increase in basal internode length; robust root architecture; increase in tiller and panicle numbers. Moreover, OsULT1-3 showed salinity tolerance with enhanced seed germination, reduced ROS content, low Na +/K + ratio in shoot and root tissue, and enhanced post-stress recovery. Collectively, our results indicate that ULT1 regulates different developmental pathways for better protection and adaptation against environmental stress.

Artificial ponds are an integral feature of the landscape in many parts of the world and contribute greatly to regional diversity. The study aimed to (1) investigate the structure and diversity of macroinvertebrate assemblages in five small permanent water bodies within a limestone quarry area, (2) determine the environmental variables shaping assemblages, and (3) evaluate changes over a relatively long period. 2. Benthic samples were collected monthly from April to November in 2009 and 2019, and basic physical and chemical water parameters were measured. Macroinvertebrates were sampled according to the time-limited and habitat-dependent sampling technique using a standard pond net. Several biocenotic indices based on taxon composition or percentage of individuals were calculated to describe the assemblages. The relationships between environmental variables and macroinvertebrates were evaluated using multivariate analyses. 3. The results showed that the assemblages were dominated by mayflies (Baetidae and Caenidae), damselflies (Coenagrionidae), and dipterans (Chironomidae), with a total of 42 taxa recorded. Over the ten-year period, taxon richness and diversity increased, especially in spring, and became more diverse among the ponds. The abundance and frequency of occurrence of hololimic organisms, such as snails (Lymnaeidae and Planorbidae), oligochaetes, and water mites, also increased. The RDA analysis indicated that temperature, seasonality, pond margin shading, and morphometry were the main environmental factors influencing macroinvertebrates, which explained 38.6% of the total variance in macroinvertebrate abundance. 4. This study indicates a significant spatial and temporal variation in macroinvertebrate distribution and assemblage structure among small artificial water bodies created in the limestone quarry, despite their proximity.

In recent decades, the demand for clean and renewable energy has grown increasingly urgent due to the irreversible alteration of the global climate. As a result, efficient organic solar cells (OSCs) have gradually gained attention as a study hotspot. To break up this dilemma, this paper reviews the molecular design strategies of benzodithiophen (BDT)-based polymer and small molecule donor materials since their birth, focusing on the development of main-chain engineering, side-chain engineering and other unique molecular design paths. Up to now, the state-of-the-art power conversion efficiency (PCE) of binary OSCs prepared by BDT-based donor materials has approached 20%. Our work detailly discusses the potential relationship between the molecular changes of donor materials and photoelectric performance in corresponding OSC devices, thereby presenting a rational molecular design guidance for stable and efficient donor materials in future.

TITLE : Genomic insights into a critically endangered island endemic songbird provide a roadmap for preventing extinction.RUNNING TITLE: King Island Scrubtit conservation genetics.AUTHORS : Ross Crates1¶, Brenton von Takach2, Catherine M Young1, Dejan Stojanovic1, Linda Neaves1, Liam Murphy1, Daniel Gautschi1, Carolyn J. Hogg3,4, Robert Heinsohn1, Phil Bell5, Katherine A. Farquharson3,4.1. Fenner School of Environment and Society, Australian National University, Linnaeus Way, Acton, Canberra 2601.2. School of Molecular and Life Sciences, Curtin University, Bentley, Perth, Western Australia 6102.3. The University of Sydney, School of Life and Environmental Sciences, NSW 2006, Australia4. Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, NSW 2006, Australia5. Biological Sciences, School of Natural Sciences, University of Tasmania, Hobart, Tasmania, 7005, Australia¶Corresponding Author: ross.crates@anu.edu.auABSTRACT: Small, fragmented or isolated populations are at risk of population decline due to fitness costs associated with inbreeding and genetic drift. The King Island scrubtit Acanthornis magna greeniana is a critically endangered endemic subspecies of the nominate Tasmanian scrubtit Acanthornis magna magna, with an estimated population of <100 individuals persisting in three patches of swamp forest. The Tasmanian scrubtit is widespread in wet forests on mainland Tasmania. We sequenced the scrubtit genome using PacBio HiFi and undertook a population genomics study of the King Island and Tasmanian scrubtit using a double-digest restriction site-associated DNA (ddRAD) dataset of 5,239 SNP loci. The genome was 1.48 Gb long, comprising 1,518 contigs with an N50 of 7.715 Mb. King Island scrubtits formed one of four overall genetic clusters, but separated into three distinct subpopulations when analysed separately. Pairwise FST values were greater among the King Island scrubtit subpopulations than among most Tasmanian scrubtit subpopulations. Genetic diversity was lower and inbreeding coefficients were higher in the King Island scrubtit than all except one of the Tasmanian scrubtit subpopulations. We observed crown baldness in 8/15 King Island scrubtits, but 0/55 Tasmanian scrubtits. Six loci were significantly associated with baldness, including one within the DOCK11 gene which is linked to early feather development. Contemporary gene flow between King Island scrubtit subpopulations is unlikely, with further field monitoring required to quantify the fitness consequences of its small effective size, low genetic diversity and high inbreeding. Evidence-based conservation actions can then be implemented before the taxon goes extinct.

Terrain and plants play a crucial role in influencing or regulating the variations of water, salt, carbon and bacteria community in the soil of coastal salt marshes. Investigating these variations and their interconnections under different conditions is essential to comprehensively understand the carbon sequestration function and reveal the underlying mechanisms of carbon sinks in coastal salt marshes. In this study, the various physical and chemical properties of rhizosphere and non-rhizosphere soil under different terrain of coastal salt marsh in Jiaozhou Bay were determined. Additionally, the contents of soil carbon components (including soil inorganic carbon, SIC; soil organic carbon, SOC; dissolved inorganic carbon, DIC; dissolved organic carbon, DOC), constituents of soil dissolved organic matter (DOM) and microbial community were also analyzed. The results showed that the contents of SIC and SOC in high-terrain soil were significantly lower than those in low-terrain soil (P＜0.05). Plants could regulate the physical and chemical properties and carbon composition of soil in different terrain, resulting in no significant difference in soil SOC contents of rhizosphere soil under different terrain. Soil DOM composition and bacterial community showed that high-terrain soil had higher bacterial diversity and bacterial activity, and plants significantly affected the bacterial community structure in soil through rhizosphere effects. The structural equation model demonstrated that terrain has an indirect impact on SOC through its influence on the fluorescence of soil DOM and bacterial diversity, and indirectly affected SIC content by altering soil water content (SWC), electrical conductivity and DOC. Plants directly or indirectly affected SOC content, and indirectly affected SIC content by changing SWC. This study contributes to a better understanding of the complex interactions between wetland ecosystems and their abiotic and biotic components, offering valuable information for wetland management and conservation efforts.

Anesthetic management of airway stent placement by rigid bronchoscopy with superior laryngeal nerve block while preserving spontaneous breathing: a case reportToshio Okada1), Mio Yoshida1), Tomoko Matsushita1), Yusuke Ishida1), Kinya Furukawa2), and Michihiro Murozono3)1) Department of Anesthesiology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan2) Department of Thoracic Surgery, Tokyo Medical University Ibaraki Medical Center, 3-20-1 Chuo, Amimachi, Inashiki-gun, Ibaraki 300-0395, Japan3) Department of Anesthesiology, Tokyo Medical University Ibaraki Medical Center, 3-20-1 Chuo, Amimachi, Inashiki-gun, Ibaraki 300-0395, JapanCorresponding author: Toshio OkadaDepartment of AnesthesiologyTokyo Medical University6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, JapanTel.: +81-03-3342-6111; Fax: +81-03-5381-6650Email: okd.shinjuku@gmail.com

Microevolutionary processes shape adaptive responses to heterogeneous environments, where these effects vary both among and within species. However, the degree to which signatures of adaptation to environmental drivers can be detected based on spatial scale and genomic marker remains largely unknown. We studied signatures of local adaptation across different spatial extents, investigating complementary types of genomic variants–single nucleotide polymorphisms (SNPs) and polymorphic transposable elements (TEs)–in populations of the alpine model plant species Arabis alpina. We coupled high-resolution (0.5m) environmental factors, derived from remote sensing digital elevation models, with whole-genome sequenced data of 304 individuals across four populations. We demonstrate that responses of A. alpina to similar amounts of abiotic variation are largely governed by local evolutionary processes and find minimally overlapping signatures of local adaptation between SNPs and polymorphic TEs. Notably, functional annotations of high-impact genomic variants revealed several defence-related genes associated with the abiotic factors studied, which could indicate indirect selective pressure of biotic agents. Our results highlight the importance of considering different spatial extents and types of genomic polymorphisms when searching for signatures of adaptation to environmental variation. Such insights provide key information on microevolutionary processes and could guide management decisions to mitigate negative impacts of climate change on alpine plant populations.

Abstract

The facial region is important for patients’ socialization. Therefore, the mutilation of this area should be rehabilitated quickly and effectively. The objective of this review was to analyze which software and equipment are most commonly used for the acquisition, manipulation of images, and printing of prototypes and facial prostheses, correlating with the type of prosthesis. The search strategy was customized for 7 different databases: LILACS, Embase, Web of Science, PubMed, Scopus, OpenGrey, and Google Scholar. Articles were selected based on inclusion and exclusion criteria, with abstract and full article readings performed independently by 2 authors, with disagreements resolved through a consensus meeting. The risk of bias for each included article was analyzed using JBI. Subsequently, data such as the type of prosthesis manufactured, software used, equipment for defect image acquisition, and printing equipment were collected. The data were tabulated, and a descriptive analysis was performed. Most studies reported the fabrication of nasal prostheses, followed by auricular and ocular prostheses. The primary technology chosen for patient defect image acquisition was computed tomography, and for image manipulation, Materialise Mimics software was commonly used. Additionally, a significant portion of the studies reported the use of direct three-dimensional printing. In conclusion, there is a significant heterogeneity among articles in the equipment and software used, and there is no clear relationship between these factors and the type of prosthesis manufactured.

Objectives：We aimed to investigated whether artificial light at night (ALN) are associated with Nasopharyngeal carcinoma (NPC) risk in Fujian province of China. Design：We use spatial epidemiology to understand the spatiotemporal distribution characteristics of nasopharyngeal carcinoma hospitalization rates, and use least squares and geographic detection models to understand the impact of nighttime light intensity on nasopharyngeal carcinoma hospitalization rates. Participants：Inpatients with NPC in 2019 were determined according to ICD-10 code and the hospitalization records of Fujian province were obtained from basic medical insurance for urban and rural residents in Fujian Province, which was provided by Fujian Medical Security Center. Variables: The main variable calculated in this study is the crude hospitalization rate Results：The NPC hospitalization rate are spatially positively correlated . The results of Ordinary Least Square (OLS) model analysis showed that ALN was positively correlated with the hospitalization rate of NPC. The R2 in the geographic weighted regression model（GWR）is greater than the OLS model, which indicates that the explanation degree of the GWR model variables on the hospitalization rate of NPC is significantly better than OLS model. Conclusions：ALN level was positively correlated with the hospitalization rate of nasopharyngeal carcinoma, which provide valuable information for the prevention and treatment strategy of NPC in Fujian province.

IntroductionMitochondrial disease (MD) is a group of disorders caused by dysfunctional mitochondria, the organelles which play an important role in the production of ATP and exist in every human cell inthe body, except for the red blood cells (1 ). Mitochondrial DNA (mtDNA) is inherited maternally and affects organs dependent on high aerobic metabolism, such as the eye, inner ear, central nervous system, skeletal and cardiac muscle. Mitochondrial diabetes mellitus (MDM), also known internationally as maternally inherited diabetes and deafness (MIDD) syndrome, is a mtDNA mutation disease, with a progressive islet β cell secretory dysfunction (2, 3 ). MIDD is associated with early onset diabetes and sensorineural deafness, but there are various other systemic features, including cardiomyopathy, renal problems, and neuropsychiatric symptoms. It was first reported by J.A. Massen et al. in 1992, who found a large pedigree with mt.3243A>G mutation, suffering from diabetes with the presence of maternal transmission, in conjunction with bilateral hearing loss in most of the carriers (4 ). From then on, there has been growing scientific evidence that a range of other point mutations in mtDNA could contribute to the pathogenesis of MIDD, such as mt-tRNA encoding genes, including MT-TI, MT-TS1, and MT-TK, and mt-proteins encoding genes, incuding MT-ND1, MT-ND4, MT-COX2, and MT-COX3. In addition, deletion and depletion of nucleotides have also been described in patients with MIDD (5 ). These novel mutations, however, are extremely rare compared to the proportion of mt.3243A>G, where there is an A to G substitution at position 3243. MIDD is currently found to be the most common type of monogenic diabetes mellitus, accounting for about 1% of all diabetes mellitus, and the incidence in China is approximately 0.6% (6 ). Because of its low incidence and irrespective of its complex clinical phenotype, MIDD is often misdiagnosed as type 1 or type 2 diabetes. Nonetheless, its treatment is different from the common types of diabetes, and incorrect diagnosis and treatment will accelerate the disease process and the occurrence of complications. Therefore, correct genetic diagnosis and treatment are crucial for patients with MIDD. This study discusses the clinical manifestations and treatment process of a typical patient with MIDD, and summarizes its clinical characteristics through a comprehensive review of the literature, with the scope of improving clinicians’ cognition of the disease and reducing misdiagnosis rate.

The effect of dendritic structure and secondary phases on the fatigue crack propagation mechanism of ERNiCrMo-3 weld metal was investigated in the present study. Element segregation in the weld metal caused severe lattice distortion in the interdendritic region, which led to the instability of crack propagation rates, resulting in considerable differences in mechanical properties between the dendrite core and interdendritic region. The limited plastic deformation capability of the interdendritic region caused the contraction of the local plastic deformation zone at the crack tip, which increased stress concentration and accelerated crack propagation. Meanwhile, element segregation promoted the precipitation of a large number of Laves pahse in the interdendritic region. The interaction between dislocations and Laves phase was enhanced as the stress increased, which generated micro-voids and provided fast channels for fatigue crack propagation. Post-weld heat treatment weakened the non-uniformity of the microstructure and the fatigue crack showed a stable propagation trend. Moreover, the dissolution of Laves phase and the precipitation of γ” enhanced the fatigue property of the weld metal.

A document by Patricia Bare. Click on the document to view its contents.

This study investigated the creep performance, fracture characteristics, and creep mechanisms of the nickel-base single crystal superalloy DD406 under conditions of 1100°C and 140MPa. The results indicate that the alloy exhibited a creep life of 104.5 hours and a strain of 33.58%. The creep curve exhibited three distinct stages: initial (deceleration), steady-state, and accelerated creep. The fracture surface was characterized by dimples and tearing edges, with no apparent slip planes. Internal to the fracture, oxidation products and recrystallized structures were observed, and the internal pores exhibited elongated shapes oriented perpendicular to the stress axis. Furthermore, analysis of the creep rate-time curve revealed three sub-stages within the initial (deceleration) creep stage, where the creep rate decreased initially, followed by an increase and subsequent decrease with time.

Offshore platforms are used for many functions such as oil and gas production, renewable energies, navigation, ship loading and unloading. Nowadays, the focus on renewable energies is increasing, hence there is need of robust design such structure which will function effectively to carry out the required function. The effect of wind and wave current can cause fatigue load on these structures. In such scenario, the joints of the offshore structure are more vulnerable to fatigue loading. Therefore, in the present research work, fatigue life evaluation of critical component of a floating wind turbine semi-submersible structure is considered. A few researchers have done work on semi-submersible floating offshore wind turbine for fatigue life evaluation. Pontoons and cross braces are the critical components of the entire substructure. Pontoons and cross braces of a 5MW wind turbine floating structure is considered for fatigue life evaluation. The substructure is modelled in Finite element software (ABAQUS V-2020). Tension and compressive load coming from wind, wave current and operational loads are considered. Stress concentration factor (SCF) for all the components is evaluated for three load cases. It is revealed that load case-1 caused the highest SCF in cross bracing. The member which has highest SCF is identified among nine connecting members of the substructure. Once the critical member is identified by highest SCF, then the cyclic load is applied for the critical member. For the member having maximum SCF and evaluated for fatigue life based on maximum stress.