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Inflammatory potential of particles from the Echinococcus granulosus laminated layer
  • Alvaro Díaz,
  • Leticia Grezzi,
  • Cecilia Casaravilla
Alvaro Díaz
Universidad de la Republica Uruguay
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Leticia Grezzi
Universidad de la Republica Uruguay
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Cecilia Casaravilla
Universidad de la Republica Uruguay

Corresponding Author:ccasarav@higiene.edu.uy

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Abstract

Cystic echinococcosis is caused by the tissue-dwelling larva (hydatid) of Echinococcus granulosus sensu lato. A salient feature is this larva is being protected by the acellular laminated layer (LL), made up of mucins and calcium inositol hexakisphosphate (Ins P 6). As the parasite grows, the LL sheds abundant particles that can accumulate in the parasite’s vicinity. Although foreign particles accumulating in tissues usually cause inflammation, the LL displays adaptations for minimising various host responses, and in vivo evidence of inflammation induced by LL particles is essentially lacking. In this work, we show that LL particles injected i.p. at a dose of 225 μg dry mass per mouse cause infiltration of eosinophils, neutrophils and monocytes/macrophages as well as disappearance of resident (large peritoneal) macrophages. The calcium Ins P 6 component was dispensable for these responses. Oxidation of the mucin carbohydrates caused decreased recruitment of neutrophils but the carbohydrate-oxidized particles caused cell influx nonetheless. The control of local granulomatous inflammation is key for survival of this larva. Therefore, our results suggest that E. granulosus must deploy mechanisms to avoid excessive local build-up of LL particles (such as targeting particles to liver Kupffer cells; Infect Immun 91:e0003123) and/or to condition the recruited cells towards immune-regulatory phenotypes.
Submitted to Parasite Immunology
08 Feb 2024Review(s) Completed, Editorial Evaluation Pending
09 Feb 2024Editorial Decision: Revise Minor
13 Apr 20241st Revision Received
17 Apr 2024Submission Checks Completed
17 Apr 2024Assigned to Editor
17 Apr 2024Review(s) Completed, Editorial Evaluation Pending
09 May 2024Editorial Decision: Accept
May 2024Published in Parasite Immunology volume 46 issue 5. 10.1111/pim.13040