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Basophil responses in susceptible AKR mice upon infection with the intestinal helminth parasite Trichuris muris
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  • Elia D. Tait Wojno,
  • Shuchi Smita,
  • Lauren Webb,
  • Bridget Mooney,
  • Simon P. Früh,
  • Oyebola Oyesola,
  • Macy K. Matheson,
  • Seth A. Peng
Elia D. Tait Wojno
University of Washington Department of Immunology

Corresponding Author:etwojno@uw.edu

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Shuchi Smita
University of Washington Department of Immunology
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Lauren Webb
University of Washington Department of Immunology
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Bridget Mooney
University of Washington Department of Immunology
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Simon P. Früh
Cornell University Baker Institute for Animal Health
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Oyebola Oyesola
University of Washington Department of Immunology
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Macy K. Matheson
University of Washington Department of Immunology
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Seth A. Peng
Cornell University Baker Institute for Animal Health
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Abstract

Intestinal helminth infection promotes a Type 2 inflammatory response in resistant C57BL/6 mice that is essential for worm clearance. The study of inbred mouse strains has revealed factors that are critical for parasite resistance and delineated the role of Type 1 versus Type 2 immune responses in worm clearance. In C57BL/6 mice, basophils are key innate immune cells that promote Type 2 inflammation and are programmed via the Notch signaling pathway during infection with the helminth Trichuris muris. However, how the host genetic background influences basophil responses and basophil expression of Notch receptors remains unclear. Here we use genetically susceptible inbred AKR/J mice that have a Type 1-skewed immune response during T. muris infection to investigate basophil responses in a susceptible host. Basophil population expansion occurred in AKR/J mice even in the absence of fulminant Type 2 inflammation during T. muris infection. However, basophils in AKR/J mice did not robustly upregulate expression of the Notch2 receptor in response to infection as in C57BL/6 mice. Blockade of the Type 1 cytokine IFN-γ in infected AKR/J mice was not sufficient to elicit infection-induced basophil expression of the Notch2 receptor. These data suggest that the host genetic background, outside of the Type 1 skew, is important in regulating basophil responses during T. muris infection in susceptible AKR/J mice.
13 Mar 2023Submitted to Parasite Immunology
13 Mar 2023Submission Checks Completed
13 Mar 2023Assigned to Editor
13 Mar 2023Review(s) Completed, Editorial Evaluation Pending
16 Mar 2023Reviewer(s) Assigned
12 Apr 2023Editorial Decision: Revise Minor
02 Jun 20231st Revision Received
02 Jun 2023Submission Checks Completed
02 Jun 2023Assigned to Editor
02 Jun 2023Review(s) Completed, Editorial Evaluation Pending
02 Jun 2023Reviewer(s) Assigned
06 Jun 2023Editorial Decision: Accept