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Gas chromatographic separation of the FAME prepared from dairy products, PHO, and refined vegetable oils applying a negative temperature gradient.
  • Pierluigi Delmonte
Pierluigi Delmonte
US Food and Drug Administration

Corresponding Author:pierluigi.delmonte@fda.hhs.gov

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Abstract

To date no single gas chromatographic method can simultaneously measure all fatty acids (FA), including trans FA (TFA), that are contained in dairy products, partially hydrogenated oils (PHO), and refined vegetable oils. Using 100% poly(biscyanopropyl siloxane) capillary columns, ruminant and dairy fats are preferentially analyzed by applying temperature programs that separate short chain FA, but not trans-18:3 from 20:1. Refined vegetable oils and PHO are preferentially analyzed by applying isothermal elutions that provide quantification of all 18 carbon TFA including trans-18:3 FA, but not of all short chain FA. In this short communication, we propose a temperature program method capable of simultaneously measuring short chain FA and all 18 carbon TFA including trans-18:3 by applying a negative temperature gradient after the elution of trans-18:1. A simplified version of the method is also described for equipment not able to perform negative temperature gradients.
16 Dec 2022Submitted to Journal of the American Oil Chemists' Society
20 Dec 2022Submission Checks Completed
20 Dec 2022Assigned to Editor
22 Dec 2022Review(s) Completed, Editorial Evaluation Pending
23 Dec 2022Reviewer(s) Assigned
09 Feb 2023Editorial Decision: Revise Minor
20 Mar 20231st Revision Received
20 Mar 2023Submission Checks Completed
20 Mar 2023Assigned to Editor
20 Mar 2023Review(s) Completed, Editorial Evaluation Pending
30 Mar 2023Editorial Decision: Accept