Conclusions
The addition of one (or two) intermediate temperature program steps to
the method developed by Golay et al [7] for the analysis of
FA in dairy products and infant formula extends the method to the
analysis of PHO and RVO. While the addition of an isothermal plateau at
184°C (method 1) achieves the separation of trans -18:3 fromc 11-20:1, the second proposed modification including a negative
temperature gradient also provided greater separation betweent 13/t 14-18:1 and c 9-18:1 (method 2).