loading page

Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
  • Gang Xiang,
  • Hans Leemhuis,
  • Marc van der Maarel
Gang Xiang
University of Groningen
Author Profile
Hans Leemhuis
Avebe Group
Author Profile
Marc van der Maarel
University of Groningen
Author Profile

Abstract

Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2,500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found between a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-sheets.

Peer review status:UNDER REVIEW

03 May 2021Submitted to PROTEINS: Structure, Function, and Bioinformatics
04 May 2021Assigned to Editor
04 May 2021Submission Checks Completed
04 May 2021Reviewer(s) Assigned