Despite growing interest in self-care to advance universal health coverage, national self-care policies are largely absent in low- and middle-income countries. We used the ExpandNet framework, which provides guidance for scaling up health interventions, to assess the environmental influences on scaling up sexual and reproductive health (SRH) self-care in Mozambique. We conducted a mixed-methods analysis that drew on data collected as part of a larger multi-phase study on self-care introduction and scale-up. Our analysis included qualitative stakeholder interviews (n=16) and a quantitative consumer survey (n=606). Summary tabulations of the quantitative survey data were coded alongside the qualitative data in an integrated analysis. Stakeholders described how self-care could complement limited primary care infrastructure and human resources but noted that inadequate public resources could hinder effective scale-up due to the need to provide follow-up services, including linkage to care and timely treatment after self-testing. Stakeholders noted the role of the private sector, particularly pharmacies, in the delivery of self-care products . Many were optimistic that community sensitization activities could address cultural barriers to SRH self-care scale-up, particularly in rural areas. Potential demand for self-care was demonstrated in the more than 85% of consumers who said they would be likely or very likely to use a home-based HPV self-sampling kit and the 72% of consumers who had never used self-injectable contraception who said they would be likely or very likely to use it in the future. This analysis of the environment for SRH self-care scale-up using the ExpandNet framework can serve as a guide to others developing self-care scale-up strategies in other settings. Key considerations for SRH self-care in Mozambique may include investments in follow-up care, integration of the private sector, implementation of a supportive social behavior change strategy, and tailored approaches to reach populations with the greatest health needs.