During the launch phase prior to microgravity stage, hypergravity and vibration can potentially damage cell models used in space medical research, compromising the success of subsequent missions. Bioinks and bioprinting play a pivotal role in the successful delivery of cell models and the fabrication of tissue models for space biomedicine research. In order to streamline the process and conserve valuable resources, it is crucial to examine the storability of bioink and high survival human cells under space conditions. This study investigated the effects of hypergravity and vibration on blood brain barrier (BBB) related cells in 2D monolayers and 3D decellularized matrices, assessing proliferation, viability, and gene expression. The study confirmed 3D embedded cells showed marked tolerance to launch-associated mechanical factors, contrasting with the disruption on 2D adherent cells. In addition, the present study provided methods for storing hybrid bioink (decellularized extracellular matrix of mammalian soft tissue) and high viability of cells loaded in bioink under the existing storage conditions of space station. Printability, rheological property and ultrastructure of bioink is maintained after storage in 4 ℃ for 4 Weeks. Three types of BBB related cells can maintain their viability and function under the cryopreservation of preservatives. This study verified the feasibility of bioink and cell storage in the low-temperature environment available on orbit, addressing the challenges of maintaining cell viability, short experimental window, and susceptibility to launch processes in cell experiments conducted in space, and providing a promising foundation on efficient and reproducible complex bioprinting in space.

From Passive Monitoring to Active Control: Aggregation-Induced Emission-Driven Antimicrobial Nanotechnology for Long-Duration SpaceflightFeiyi Sun1,2, Nuomin Li1,3, Zheng Zhao2, Zicong Zhang2, Zuowan Zhou4,5, Xiaoling Xu4,5, Congwen Liu4, Qihuan Xiong6, Jianmin Tang6, Shiyong Yu7, Ying Zhang8, Ben Zhong Tang2,9,*, Yulin Deng3,*Dr. F.Sun, Dr. N. Li1Department of Comprehensive Basic Experiment, The Ninth Medical Center of Chinese PLA General Hospital, Beijing, 100101, China.Dr. F. Sun, Prof. Z. Zhao, Dr. Z. Zhang, Prof. B. Z. Tang2Guangdong Basic Research Center of Excellence for Aggregate Science, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, ChinaDr. N. Li, Prof. Y. Deng,3School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.Prof. Z. Zhou, Prof. X. Xu, Dr. C. Liu,4School of Chemistry, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.Prof. Z. Zhou, Prof. X. Xu5Key Laboratory of Advanced Technologies of Materials (Ministry of Education), Southwest Jiaotong University, Chengdu, Sichuan 610031, China.Mr. Q. Xiong, Mr. J. Tang6Changsha XiangJi-Hiden Technology Co., Ltd, Changsha, Hunan 410000, ChinaDr. S. Yu7Beijing Genshu Technology Co., Ltd, Beijing 100041, ChinaProf. Y. Zhang8Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.Prof. B. Z. Tang9Department of Chemistry, Department of Chemical and Biological Engineering, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Kowloon, Hong Kong 999077, China.Correspondence: Ben Zhong Tang (tangbenz@cuhk.edu), Yulin Deng (deng@bit.edu.cn)Keywords: Tianzhou-9, Aggregation-induced emission, China space station, Antibacterial module