Indonesia is richness biodiversity country, including a wide variety of vertebrate and plants species. However, assessing biodiversity in tropical rainforest remains challenging itself. The use of conventional tools has commonly been employed for monitoring and research purposes. Invertebrate-derived DNA (iDNA), a subdiscipline of environmental DNA (eDNA), has emerged as a noninvasive tool that complements traditional methods for biodiversity assessment. It enables the detection of vertebrate species and the monitoring of their populations through molecular approaches. Leveraging abundant haematophagous leeches as a tool offers a promising approach to accessing a wider range of hosts inhabiting an area and retain high-quality host DNA in their guts for extended periods. Using Sanger sequencing with five primer sets (16Scp, 16Sed, 12S, ND2, and RepCOI) designed to target broad taxonomic groups, 272 Haemadipsa spp. samples were successfully amplified, resulting in the identification of 17 unique vertebrate hosts, including mammals, amphibians, and reptiles. Within our 16Sed results, we noted that the primer sets captured a broader range of taxa, encompassing both mammals and reptiles, thereby enhancing species richness detection. Notably, we present evidence the first iDNA-based detection of the rare blue-eyed litter frog, Leptobrachium waysepuntiense, from western Sumatra. Therefore, this study suggests that the use of haematophagous leeches represents a promising approach for biodiversity monitoring in Indonesia. This method offers a complementary strategy that can be integrated with existing practices to strengthen conservation efforts.