Antibiotic resistance is increasing worldwide so alternative of commercial antibiotics should be in choice. S tevia rebaudiana (stevia) is a member of the Asteraceae family which contains potential antimicrobial agents to inhibit the growth of pathogenic microbes. Thus this study aims to evaluate the antimicrobial properties of S tevia rebaudiana against the multidrug resistant bacteria. Time-kill assay was performed to observe the efficacy of the extracts against pathogenic microbes. The agar well diffusion method was introduced to obtain the antibacterial activity of ethanol and methanol extract of stevia powder. The MIC was determined through 96-well plate technique. of leaves using solvents and the anti-bacterial activity was performed through. The methanolic extract of Stevia showed higher antibacterial activity as compared to ethanolic extract against all the bacterial strains. The highest zone of 18 mm was noticed against gram positive Staphylococcus aureus while the lowest zone 15 and 11mm was observed agasint Serretia mercengens and Salmonella typhymurium respectively. The ethanolic extract produced the zone diameter within the range of 8-13mm against the tested bacteria. MIC values were 1mg/ml was recorded for all the bacterial strains while MBC was 2mg/ml. All the bacterial growth was significantly killed within 15hrs accept Salmonella typhymurium and E. coli. The results of present study indicate that the stevia leaf extracts have inhibitory efficacy against microorganisms and further study will reveal the possibility of employing them in medicines for the treatment of infectious diseases caused by the test microorganisms.