Objective: To evaluate the validity and reliability of a modified Knowledge, Attitude, Practice and Confidence (KAPC) survey designed to assess nurses’ and midwives’ preparedness to care for women with female genital mutilation/cutting (FGM/C). Design: Pre-test-post-test study Setting: South Australia Sample: Five clinical and academic experts contributed to the validity assessment. Fifteen South Australian nurses and midwives participated in the test-retest reliability analysis. Method: A 50-item survey was developed through an integrative literature review and consultation with FGM/C clinical experts and academics. Content and face validity were assessed against three criteria: relevance, clarity and comprehension. Test-retest reliability was used to determine the stability of responses over time. Main outcome measures: Content and face validity (assessed using Gwet’s AC) and test-retest reliability (assessed using intraclass correlation coefficients (ICC)) across four constructs: knowledge, attitude, practice and confidence. Results: The KAPC survey demonstrated strong content validity (Gwet’s AC = 0.92, p <0.001) and face validity (Gwet’s AC = 0.95, p <0.001). The survey demonstrated satisfactory test-retest reliability across all four constructs: knowledge (ICC = 0.930, 95% CI: 0.823, 0.974), attitude (ICC = 0.622, 95% CI: 0.305, 0.860), practice (ICC = 0.764, 95% CI: 0.501, 0.912) and confidence, (ICC = 0.766, 95% CI: 0.506, 0.913). Conclusion: The findings support the KAPC survey as a valid and reliable instrument for assessing Australian midwives’ and nurses’ knowledge, attitudes, practice and confidence in the context of FGM/C care.