Antimicrobial resistance is a growing issue worldwide, directly contributing to more than one million deaths each year. Developing countries like Uganda rely on the continued effectiveness of antibiotics to combat endemic and epidemic diseases. This study investigated antimicrobial resistance awareness and perceived responsible antibiotic use among members of the Luwero district in Uganda. Using an electronic survey administered in person by Luwero district teams, data collection included variables reflecting aspects of antibiotic stewardship such as how often antibiotics should be taken, dose completion, and rational use. Results indicated that 6% of respondents correctly defined antimicrobial resistance and 31% stopped taking antibiotics when they felt better. There was no significant relationship found between level of education and the variables of interest. Further investigation is necessary to more fully illuminate antibiotic use and stewardship in this region. To increase awareness, it is recommended that antimicrobial resistance education should be introduced at the primary or secondary education level.