This study investigated the impact of Heartfulness Meditation (HM) on alpha power modulation in individuals with varying meditation experience. Thirty-three participants were grouped into long-term meditators (LTM, n = 12), short-term meditators (STM, n = 11), and nonmeditator controls (CG, n = 10). EEG data were recorded using a 129-channel system across four experimental conditions: baseline, meditation, transmission, and postrest. Signals were preprocessed through a rigorous pipeline including bandpass filtering (8–12 Hz), artifact removal via RANSAC and ICA, and alpha power estimation using Welch’s method. Linear mixed-effects modeling revealed a significant three-way interaction between Group, Phase, and Region (p < 0.001), indicating that alpha power modulation varied by brain region and meditation phase. Follow-up contrasts showed that both LTM and STM groups exhibited significantly greater alpha power than controls, particularly in the frontal, parietal, and occipital regions during meditation and postrest phases. These effects were most prominent during the early meditation phase (M1) and sustained across transmission and postmeditation states. Effect size analyses confirmed moderate-to-large group differences (d = 0.52–0.65), with the strongest effects observed in the right parietal and frontal cortices. These findings suggest that regular HM practice enhances alpha activity associated with relaxation, attentional regulation, and emotional processing. The study supports the potential of Heartfulness Meditation as a nonpharmacological approach to promoting cognitive and affective well-being.