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\begin{document}

\title{Radiolabeling of NOTA-CTHRSSVVC peptide using
{[}\textsuperscript{18}F{]}AlF}



\author[1]{SRSTT Radiopharmaconnect}%
\affil[1]{Radiopharmaconnect}%


\vspace{-1em}



  
  \date{April 24, 2024}


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\textbf{}\selectlanguage{english}
\begin{figure}[H]
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\includegraphics[width=0.70\columnwidth]{figures/CAFACHEM/CAFACHEM}

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\textbf{\href{https://www.srsweb.org/cafachem2020}{\textbf{\textbf{CAFACHEM~2020}}}
\textbf{}} organizers are grateful for the support
of\textbf{\textbf{~}\href{https://www.pmod.com/web/}{\textbf{\textbf{\textbf{PMOD}}}}}

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\includegraphics[width=0.70\columnwidth]{figures/pmod/pmod}

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\textbf{Authors: Carlos Alberto Rossatto Jr}\textsuperscript{1},
Rosemeire Aparecida Silva\textsuperscript{2}, Ademar Benevolo
Lug\selectlanguage{ngerman}ão\textsuperscript{3}, Roger Chammas\textsuperscript{1}, Carlos
Alberto Buchpiguel\textsuperscript{1}, Fabio Luiz Navarro
Marques\textsuperscript{1}.

1-Faculdade de Medicina da Universidade de Sao Paulo, Brazil

2-Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas da
Faculdade de Medicina da Universidade de Sao Paulo, Brazil

3- Instituto de Pesquisas Energeticas e Nucleares, Brazil

\par\null

\textbf{\textbf{Background}}

\textbf{\textbf{}} {[}\textsuperscript{18}F{]}Fluorine radiolabeled
peptides has been investigated as a diagnostic tool. However, time
consuming and low yield reaction using prosthetic groups is the
challenging for these compounds. We found that
\textsuperscript{111}In-DOTA-CTHRSSVVC, was able to bound tissues
containing atherosclerotic plaques\textsuperscript{1}, and radiolabeling
it with {[}\textsuperscript{18}F{]}AlF will help the investigation of
molecular imaging studies. \textbf{\textbf{}}

\par\null

\textbf{\textbf{Aims}}

\textbf{\textbf{}} The aim of this work was to evaluate the parameters
for radiolabeling the NOTA-CTHRSSVVC peptide using
{[}\textsuperscript{18}F{]}AlF complexation methodology~.

\par\null

\textbf{Methods}

\textbf{} NOTA-CTHRSSVVC was diluted to 1.0 mg/mL in NaAcO buffer (0.2M;
pH 4.0). In a vial containing 80 \selectlanguage{greek}µ\selectlanguage{english}L of peptide solution, was added 20 \selectlanguage{greek}µ\selectlanguage{english}L
of AlCl\textsubscript{3} solution (40mM) followed by 100-200 \selectlanguage{greek}µ\selectlanguage{english}L of
{[}\textsuperscript{18}F{]}fluoride solution (37-92.5
MBq).\textsuperscript{2} The reaction was heated at 90-100 \selectlanguage{ngerman}°C for 10-20
min and analyzed by HPLC.

\par\null

\textbf{Results and Conclusion}

\textbf{} HPLC analysis showed radiochemical yield of 66.74±0.26 \%
(n=3) and after purification radiochemical purity of 95.46±1.12\% (n=3).
The compound was stable for at least 4 hours, demonstrating the
possibilities to apply {[}\textsuperscript{18}F{]}AlF-NOTA-CTHRSSVVC~ to
in vitro studies. This work showed that was possible to obtain a new
radiotracer with good yield.\textsuperscript{3}

\textsuperscript{} \textbf{}

\textbf{References}

\textbf{1.}~Silva R et al. ~CTHRSSVVC Peptide as a Possible Early
Molecular Imaging Target for Atherosclerosis. Int J Molecular Science.
2016;17:1383-1400.

\textbf{2.}~Laverman, P et al. A Novel Facile Method of Labeling
Octreotide with~\textsuperscript{18}F-Fluorine. The J of Nucl Med 2010;
51(3):454-461.

\textbf{3.} \emph{} Hausner, SV et al. In Vitro and In Vivo Evaluation
of the Effects of Aluminum {[}\textsuperscript{18}F{]}Fluoride
Radiolabeling on an Integrin
\selectlanguage{greek}α\selectlanguage{english}\textsubscript{v}\selectlanguage{greek}β\selectlanguage{english}\textsubscript{6}-Specific Peptide. Nucl Med and
Biol. 2014;41:43-50.

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\includegraphics[width=0.70\columnwidth]{figures/Screenshot-2020-07-10-at-12-20-47/Screenshot-2020-07-10-at-12-20-47}

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