Abrus precatorius a herbaceous flowering plant belonging to the Fabaceae (bean) family is extensively utilized in traditional medicine across India and tropical and subtropical regions of Asia, including China, Indonesia, and parts of South Africa. This study focuses on extracting oil using purified A. precatorius seeds, Eclipta prostrata leaves (Karisalankanni keerai), and sesame oil. The formulated oil was assessed for its physical properties, including a density of 0.88 g/cm³, 0.41% moisture content, 0.8820 specific gravity, and 0.01% ash content. Additionally, its physicochemical characteristics were analyzed, revealing a free fatty acid content of 2.96%, an iodine value of 58.3 mgI₂/g, a peroxide value of 4.2 mEq/kg, an acid value of 5.90 mgKOH/g, a refractive index of 1.4620 at 40°C, a saponification value of 221 mgKOH/g, and a total phenolic content of 1.5 mgGAE/g. The obtained results were compared with those of other oils, and the extracted oil was further examined for its total fatty acid composition and the formulated oil comprises 99.43 g of total fat per 100 g, with monounsaturated fatty acids (MUFA) constituting 42.05%, polyunsaturated fatty acids (PUFA) 40.42%, saturated fatty acids (SFA) 16.96%, and trans-fatty acids (TF) present in negligible amounts (<0.05%). Additionally, its shelf life was evaluated to ensure its quality and stability.