Agaricomycetes is a class of wood-decay fungi known for their enzymatic production, which has been studied for several biotechnological purposes, such as degradation of recalcitrant and xenobiotic compounds using laccases and phenoloxidases. However, the full biotechnological potential of Agaricomycetes has not yet been fully explored, even less of Brazilian species. Therefore, the present study aimed to analyze and compare the tannase, amylase and cellulase production and capacity of Brazilian Agaricomycetes isolates under the presence of carbon-based nanoparticles (Cdots) enriched or not by Fe 3+, Cu 2+ and Mn 2+. The isolates were evaluated according to the assimilation and biostimulation tests carried out in solid medium, added with 2mg/mL of Cdots. The production of tannases, cellulases and amylases were detected for Psilocybe sp., Schizophyllum commune, Trametes flavida and Trametes sanguinea isolates. In addition, Fe 3+ and Cu 2+ were identified as promising and cheaper medium enrichers, while Mn +2 has shown inhibitory activity.