This study investigates the distribution of fingerprint minutiae types in boys with autism spectrum disorder (N = 48) compared to neurotypical controls (N = 46), with the aim of gaining insights into atypical developmental processes. The analysis focused on differences in the total number and types of minutiae across two areas of the fingerprint. Results showed no significant difference in the total number of minutiae across all fingers; however, notable differences were observed on the fourth finger (p = 0.001573) and in specific minutiae types. Boys with ASD showed a higher incidence of types like fragments, dots, and breaks in the central area (p < 0.00001), while neurotypical boys exhibited more enclosures. These findings suggest that the distribution and types of fingerprint minutiae could be potential markers for neurodevelopmental differences, as they indicate a disruption in the normal developmental pathways.