Abstract Objective: This study assessed cervical cancer screening uptake and its associated factors among reproductive-age women in Jimma university medical centre. Design, Setting and Population: A community-based cross-sectional study was conducted in Jimma University Medical Centre among randomly selected 905 reproductive-age women. Method and outcome measures: Data were collected using face-to-face interviews using a structured questionnaire. Descriptive summaries of data were done using descriptive statistics. Binary logistic regression was used for the analytical study. Adjusted odds ratio and 95% confidence interval were respectively used to measure statistical associations and their statistical significance. Results: 905 women participated in the study making the response rate 96.79%. Only 83 (9.2%) women had ever been tested for cervical cancer. Being Muslim (OR=0.36, 9% CI; 0.15-0.88), not being head of household (OR= 0.23, 95% CI; 0.08-0.67), lack of knowledge of cervical cancer (OR=0.26, 95% CI; 0.09-0.74), lack of a favorable attitude towards cervical cancer screening (OR=0.13, 95% CI; 0.04-0.49), not listening to radio (OR=0.23, 95% CI; 0.07-0.74) were negatively associated with uptake of cervical cancer screening, whereas, none ever use of contraceptive (OR=2.92; 95% CI: 1.10-7.72) was positively associated with uptake of the screening. Conclusion: The rate of cervical cancer screening uptake from the current study is unacceptably low. Streamlining and strengthening curriculum integration and extracurricular health education intervention on cervical cancer giving due emphasis to Muslim-dominated communities and contraceptive users are recommended. Due emphasis should also be given to mass-media expansions and increasing coverage of health education to promote cervical cancer screening. Funding: None