backend=biber, style=alphabetic, sorting=ynt ]biblatex 2.5. Obtention, Preparation and Counting of Splenic Cell Suspensions
Fourteen days post-infection, mice were anesthetized (ketamine 50 mg/kg and xylazine 25 mg/kg) and euthanized. Spleens were removed, macerated in RPMI-1640 medium, and subjected to centrifugation (400×g, 10 min, 4°C). Red blood cells were lysed using sterile water and PBS 10X. After washing and fat removal, leukocytes were resuspended in PBS with 5% FBS. Viable and non-viable cells were differentiated using Trypan Blue, counted in a Neubauer chamber, and adjusted to 1×10⁷ cells/mL.