backend=biber, style=alphabetic, sorting=ynt ]biblatex 2.7. Trypanosoma cruzi Epimastigote Antigen (EPI) Preparation
EPI was prepared from stationary-phase Y strain epimastigotes cultivated in LIT medium. After three freeze-thaw cycles and sonication, the lysate was centrifuged (37,000×g, 90 min), dialyzed against PBS, sterilized through 0.22 μm filters, and stored at −70°C. Protein content was determined by Lowry’s method (Lowry et al. 1951).