Background: High-fat diets (HFD) generate free radicals causing oxidative stress posing risks of developing various diseases. Objective: The study determined the effects of hexane (HF) and water fractions (WF) of Vigna subterranean (VS) on HFD-induced oxidative stress Wistar rats. Method: VS seeds were processed into powder, extracted in absolute ethanol and fractionated using hexane, chloroform, ethyl acetate, and water. Forty-nine adults male Wistar rats were grouped into seven of seven rats each. Group 1 (Normal control) received normal pelleted diets (NPD) and distilled water; groups 2-7 rats were induced with HFD for 12 weeks. Thereafter, rats were administered; groups; 2 (HFD control+ distilled water), 3 (HFD + 100 mg/kg orlistat), 4 (HFD + 200 mg/kg HF), 5 (HFD + 400 mg/kg HF), 6 (HFD + 200 mg/kg WF), 7 (HFD + 400 mg/kg WF) daily orally for 4 weeks. Biochemical, hematological, oxidative stress markers, histology (liver and kidney) was performed using standard methods. Results: HF and WF significantly reduced (p<0.05) food intake, weight gain, lipid profiles, liver enzymes, renal parameters, MDA and increase SOD, CAT, GSH without inflammation in liver and kidney compared to HFD controls. Conclusion: VS fractions may mitigate hyperlipidemia, oxidative stress, and inflammation without significant adverse effects. The findings suggest further research on pharmacological mechanisms.