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Sanhua Fang
Sanhua Fang

Public Documents 1
Super-Resolution Radial Fluctuations (SRRF): A Versatile and Accessible Tool for Live...
Sanhua Fang
Li Liu

Sanhua Fang

and 4 more

March 17, 2025
To break through the diffraction limit of light, various super-resolution techniques have been developed. Super-resolution Radial Fluctuations (SRRF) is an emerging super-resolution microscopy technique that utilizes a standard wide-field fluorescence microscope and open-source software plugins compatible with ImageJ. As a result, SRRF has relatively low hardware and software requirements, making it highly accessible to researchers. Here we first introduce the basic principles and workflow of SRRF, then describe the open-source ImageJ software tools NanoJ-SRRF and NanoJ-SQUIRREL. NanoJ-SRRF is used for reconstructing super-resolution images, while NanoJ-SQUIRREL is used for quantitatively assessing the quality of super-resolution images. Next, We summarize the advantages and disadvantages of SRRF as well as optimization methods. Finally, we present the applications of SRRF technology, along with potential avenues for future technical improvements.

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