The study investigated the spatial distribution of Straw-Colored Fruit Bat (Eidolon helvum) roosts within the Obafemi Awolowo University (OAU) campus in Nigeria. Using field surveys and GIS analysis, the researchers mapped bat roost distribution, identified hotspots, and explored tree species preferences. Key findings include pronounced roosting clusters, particularly in the Faculty of Science and Faculty of Administration areas, with Celtis zenkeri identified as the most preferred roosting tree species. The study emphasizes the importance of specific tree attributes, such as height and canopy area, in roost site selection within the urban campus environment. The research contributes to a better understanding of bat roosting ecology and provides insights to guide conservation strategies, recommending that urban planners prioritize the preservation of preferred tree species like Celtis zenkeri.