A robust method was developed and validated for determining 107 pesticide residues in livestock and poultry meat. The extraction process utilized acetonitrile in a citric acid buffer environment, followed by clean-up through multi-plug filtration. Separation was achieved using gas chromatography on an HP-5MS capillary column with a programmed temperature gradient, and detection was performed via mass spectrometry in multiple reaction monitoring modes. The results showed that the linear ranges of 107 analytes exhibited strong correlation coefficients, all exceeding 0.9901, with limits of quantification spanning from 1.0 to 15.2 μg/kg. The average recoveries and relative standard deviations for 94 analytes ranging from 70.2% to 120.0% and 4.1% to 15.9%, respectively, at the three different spiked levels. This method was applied to analyze 132 batches of livestock and poultry meat, detecting no pesticides, thereby demonstrating its simplicity, reliability, reproducibility, and suitability for the detection of pesticides in meat samples.