Comparison of Biosampling Efficiency and Fish Assemblages between Crustacean Species
An average of 4.6 fish species were detected per digestive sample, with significant variation in biosampling efficiency among crustacean species (Table 1, Figure 1). The most abundant shrimp species,Macrobrachium olfersii and Macrobrachium brasilience , were the most efficient biosamplers, detecting 51 and 36 fish species respectively, with high detection rates (6.36 and 4.50 fish species per digestive sample in average). Together, they captured more than 94% of the total dDNA fish diversity (Figure S4). In comparison, the biosampling efficiency of the three other shrimp was much lower, with fewer than three fish species per specimen on average. Species accumulation curves (Figure 1) support this finding, showing faster accumulation rates for M. olfersii and M. brasilience compared to the other species.