Background: Atopic Dermatitis (AD) is a chronic condition characterised by recurrent skin lesions and itch, significantly impacting patients’ quality of life. AD is most common during childhood, with global prevalence ranging between 10% and 20%. Objective: Identifying protein biomarkers underlying AD in children, to better understand the pathogenesis. Methods: Cross-sectional study using untargeted tandem mass spectrometry to analyse skin tape protein extracts from 71 10-year-old Singaporean children. Subjects were stratified into three groups, no history of AD (NoAD=29), history of AD but no symptoms at time of sample collection (PastAD=22) and present AD (PresentAD=20). Significant proteins were shortlisted and functional analyses were performed. Results: 397 differentially expressed proteins were identified (p-value<0.05) with 18 proteins shared with a validation cohort. Ingenuity pathway analysis shows that ‘PastAD vs PresentAD’ share common pathways with ‘NoAD vs PresentAD’. The top pathways obtained are related to inflammatory responses, such as neutrophil degranulation, JAK-STAT signalling and interleukin-1 family signalling. Conclusions: Multiple protein biomarkers and pathways, including keratinization, inflammatory responses and oxidative stress response, are involved in the onset and persistence of AD. Despite resolution of disease manifestations in PastAD subjects, functional analyses show that some pathways and biomarkers indicative of AD persist.