The 14 paralogs of the POTE gene family are distributed across eight distinct chromosomes in humans. The POTEE (POTE ankyrin domain family member E) has been shown to express in several human malignancies, including colon, prostate, lung, breast, ovarian, and pancreatic cancers. This study aimed to raise POTE2B (isoform 2 of POTEE protein) polyclonal antibodies in BALB/c mice to characterise and investigate the early detection of human POTEE protein in malignant cells. To generate a specific pol