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\begin{document}
\begin{CJK}{UTF8}{gbsn}

\title{The Isme Journal Template}



\author[1]{Josh Nicholson}%
\affil[1]{Authorea}%


\vspace{-1em}



  
  \date{April 25, 2026}


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\selectlanguage{english}
\begin{abstract}
Original Articles must be prepared with an unstructured abstract
designed to summarise the essential features of the paper in a logical
and concise sequence.%
\end{abstract}%



\sloppy


Please note that original articles must contain the following
components. Please see below for further details.

\begin{itemize}
\tightlist
\item
  Cover letter
\item
  Title page (excluding acknowledgements)
\item
  Abstract
\item
  Introduction
\item
  Materials (or Subjects) and Methods
\item
  Results
\item
  Discussion
\item
  Acknowledgements
\item
  Conflict of Interest
\item
  References
\item
  Figure legends
\item
  Tables
\item
  Figures
\end{itemize}

Reports of clinical trials must adhere to the registration and reporting
requirements listed in theEditorial Policies.

\subsection*{Cover Letter:}

{\label{921769}}

The uploaded covering letter must state the material is original
research, has not been previously published and has not been submitted
for publication elsewhere while under consideration. If the manuscript
has been previously considered for publication in another journal,
please include the previous reviewer comments, to help expedite the
decision by the Editorial team. Please include a Conflict of Interest
statement.

\subsection*{Title Page:}

{\label{966614}}

The title page should bear the title of the paper, the full names of all
the authors and their affiliations, together with the name, full postal
address, telephone and fax numbers and e-mail address of the author to
whom correspondence and offprint requests are to be sent (this
information is also asked for on the electronic submission form). The
title page must also contain a Conflict of Interest statement (see
Editorial Policy section).

\begin{itemize}
\tightlist
\item
  The title should be brief, informative, of 150 characters or less and
  should not make a statement or conclusion.
\item
  The running title should consist of no more than 50 letters and
  spaces. It should be as brief as possible, convey the essential
  message of the paper and contain no abbreviations.
\item
  Authors should disclose the sources of any support for the work,
  received in the form of grants and/or equipment and drugs.
\item
  If authors regard it as essential to indicate that two or more
  co-authors are equal in status, they may be identified by an asterisk
  symbol with the caption `These authors contributed equally to this
  work' immediately under the address list
\end{itemize}

\subsection*{Subject Categories:}

{\label{339602}}

The Subject Categories are used to structure the current and archived
online content of The ISME Journal and to help readers interested in
particular areas of microbial ecology find relevant information more
easily. Subject Categories are also indicated in the table of contents
and on the title page of the published article. Authors should suggest
an appropriate Subject Category for the submitted manuscript. One
category may be selected from the following list:

\begin{itemize}
\tightlist
\item
  Microbial population and community ecology
\item
  Microbe-microbe and microbe-host interactions
\item
  Evolutionary genetics
\item
  Integrated genomics and post-genomics approaches in microbial ecology
\item
  Microbial engineering
\item
  Geomicrobiology and microbial contributions to geochemical cycles
\item
  Microbial ecology and functional diversity of natural habitats
\item
  Microbial ecosystem impacts
\end{itemize}

\section*{Materials/Subjects and
Methods:}

{\label{408525}}

This section should contain sufficient detail, so that all experimental
procedures can be reproduced, and include references. Methods, however,
that have been published in detail elsewhere should not be described in
detail. Authors should provide the name of the manufacturer and their
location for any specifically named medical equipment and instruments,
and all drugs should be identified by their pharmaceutical names, and by
their trade name if relevant.

\section*{Results and Discussion:}

{\label{995101}}

The Results section should briefly present the experimental data in
text, tables or figures. Tables and figures should not be described
extensively in the text, either. The discussion should focus on the
interpretation and the significance of the findings with concise
objective comments that describe their relation to other work in the
area. It should not repeat information in the results. The final
paragraph should highlight the main conclusion(s), and provide some
indication of the direction future research should take.

\section*{Acknowledgements:}

{\label{286608}}

These should be brief, and should include sources of support including
sponsorship (e.g. university, charity,~commercial organisation) and
sources of material (e.g. novel drugs) not available commercially.
Conflict of Interest: Authors must declare whether or not there are any
competing financial interests in relation to the work described.
Thisinformation must be included at this stage and will be published as
part of the paper. Conflict of interest should be noted in the cover
letter and also on the title page. Please see the Conflict of Interest
documentation in the EditorialPolicy section for detailed information.

\section*{References:}

{\label{854014}}

Only papers directly related to the article should be cited. Exhaustive
lists should be avoided.References should follow the Harvard format. In
the text of the manuscript a reference should be cited by author and
year of publication e.g. (Bailey \& Kowalchuk 2006) and (Heidelberg et
al 1994) and listed at the end of the paper in alphabetical order of
first author. References should be listed and journal titles abbreviated
according to the style used by Index Medicus examples are given below.
All authors should be listed for papers with up to six authors; for
papers with more than six authors the first six only should be listed
followed by et al. Abbreviations for titles of medical periodicals
should conform to those used in the latest edition of Index Medicus. The
first and last page numbers for each reference should be provided.
Abstracts and letters must be identified as such. Papers in press may be
included in the list of references. Personal communications must be
allocated a number and included in the list of references in the usual
way or simply referred to in the text;the authors may choose which
method to use. In either case authors must obtain permission from the
individual concerned to quote his/her unpublished work.





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