Pigs have been improved throughout decades by increasing meat content in the carcasses. However, this has led to a reduction in fat levels, particularly intramuscular fat (IMF), which is crucial for flavor. The current study, utilized combined RNA-ATAC sequencing analyses to identify possible key transcription factors (TFs) that regulate important molecular mechanisms in the liver associated with fat deposition in pig bodies. In the study were used native pigs Złotnicka White, which is not under selection pressure. RNA-seq identified 272 genes as differentially expressed (DEGs, showing >1.2 FC). ATAC-seq identified 6,333 significant peaks (DAR – differentially accessible regions) in TSS flanking region. 98 genes were overlapped between RNA and ATAC seq data and these significant DEGs-DARs were further included into motif analysis. TRRUST and MEME tools were used to identify crucial TFs, which predicted possible binding TF motifs based on transcription start site ATAC signals. The candidate TFs for fat deposition in pigs suggested in the present study are SREBP1, ATF4, KLF11, RORA and MYC, which were enriched due to TSS ATAC signals. Moreover, for TFs MYC, ATF4 and, KLF11, ATAC signals within transcription start site flanking regions were identified.