Browntop millet (Brachiaria ramosa L. Stapf) holds cultural and agricultural significance in Southern India, serving as both a staple food for humans and fodder for livestock. Recently, its high fiber and protein content has gained public attention, making it popular as a nutritious grain. However, despite its nutritional benefits, the limited genomic information available for browntop millet has hindered breeding efforts. To address this knowledge gap, an assessment was made to test the transferability of SSR (Simple Sequence Repeat) primers from finger millet to browntop millet, aiming to identify genetic polymorphism. In this study, 27 accessions of browntop millet were subjected to SSR analysis using 100 SSR primers developed for finger millet. Among the 100 primers tested, five were mono-nucleotide, 38 were di-nucleotide, 42 were tri-nucleotide, and 15 were tetra-nucleotide repeats. Initial screening of 100 primers on 100 primers on two browntop millet accessions GPUBT-2 and IC617953 showed 40 per cent and 42 per cent transferability. Among the 30 amplified primers screened on both browntop millet genotypes, one was a mono-nucleotide repeat, 16 were di-nucleotide repeats, and 13 were tri-nucleotide repeats. The analysis revealed that di-nucleotide repeats were the most common repeat type. 30 primers which showed amplification in both genotypes were used for screening 27 browntop millet genotypes. The PIC values of all the polymorphic loci for 25 browntop millet genotypes varied from 0 to 0.68, major allele frequency varied from 0.40 to 1, allele number varied from 1 to 5 and genetic diversity varied from 0 to 0.72. Cluster analysis combining morphological and molecular markers based on UPGMA method resulted in two major clusters. These findings demonstrate the potential for utilizing genomic resources from related millet species and highlight opportunities to enhance browntop millet breeding programs.