Figure 1. TP53BP2 was upregulated in placental trophoblasts from PE pregnancies. (A) KEGG signaling pathway
histogram. (B) Cluster heatmap showing the top 20 autophagy-related DEGs associated with autophagy (|fold change|≥2.0,P ≤0.05) in placentas from PE pregnancies and non-PE pregnancies.
The red and blue strips indicate upregulated and downregulated genes,
respectively. (C) Box plot illustrating the expression
of the top 20 DEGs in placentas from PE pregnancies and non-PE
pregnancies. The blue box plot represents PE pregnancies, and the red
box plot represents non-PE pregnancies. (D) The expression of
TP53BP2 in placentas was detected via western blotting (PC,
n=40; PE, n=45 ). (E) Representative immunohistochemical
staining of TP53BP2. Scale bar=200 µm. (F) Double
immunofluorescence staining showing colocalization of TP53BP2 (red) and
CK-7 (trophoblast marker, green). Nuclei were stained with DAPI (blue).
Scale bar=50 µm. (G) Autophagosomes in HTR8/Svneo and JEG-3
cells transfected with Ad-TP53BP2 or sh-TP53BP2 under hypoxia were
observed via transmission electron microscopy (TEM). Scale bar=1000 nm.(H) Representative images of GFP-RFP-LC3 staining in HTR8/Svneo
and JEG-3 cells transfected with sh-TP53BP2 under hypoxia
(n= 3). Scale bar=20 µm. (I) The expression of LC3B-II
and p62 in HTR8/Svneo and JEG-3 cells transfected with Ad-TP53BP2 or
sh-TP53BP2 under hypoxia was detected by western blotting
(n= 3). The data are presented as the means ± SDs.**P <0.01,***P <0.001.