Quantitative real-time polymerase chain reaction (qRT-PCR)
analysis
Total RNA was isolated from human placentas and cells via an
RNA isolation kit (TIANGEN, Beijing, China) following the manufacturer′s
instructions. Following quantification of the RNA, cDNA was
synthesized via a reverse transcription kit (Takara, Tokyo, Japan). The
cDNA was subsequently subjected to real-time PCR amplification in a
thermal cycler via TBGreen Fast qPCR mix (Takara, Tokyo, Japan). All
experiments were performed in triplicate, and the data were normalized
to those of GAPDH. The specific primer sequences are provided in Table
S1.