Figure 1. TP53BP2 was upregulated in placental trophoblasts from PE pregnancies. (A) KEGG signaling pathway histogram. (B) Cluster heatmap showing the top 20 autophagy-related DEGs associated with autophagy (|fold change|≥2.0,P ≤0.05) in placentas from PE pregnancies and non-PE pregnancies. The red and blue strips indicate upregulated and downregulated genes, respectively. (C) Box plot illustrating the expression of the top 20 DEGs in placentas from PE pregnancies and non-PE pregnancies. The blue box plot represents PE pregnancies, and the red box plot represents non-PE pregnancies. (D) The expression of TP53BP2 in placentas was detected via western blotting (PC, n=40; PE, n=45 ). (E) Representative immunohistochemical staining of TP53BP2. Scale bar=200 µm. (F) Double immunofluorescence staining showing colocalization of TP53BP2 (red) and CK-7 (trophoblast marker, green). Nuclei were stained with DAPI (blue). Scale bar=50 µm. (G) Autophagosomes in HTR8/Svneo and JEG-3 cells transfected with Ad-TP53BP2 or sh-TP53BP2 under hypoxia were observed via transmission electron microscopy (TEM). Scale bar=1000 nm.(H) Representative images of GFP-RFP-LC3 staining in HTR8/Svneo and JEG-3 cells transfected with sh-TP53BP2 under hypoxia (n= 3). Scale bar=20 µm. (I) The expression of LC3B-II and p62 in HTR8/Svneo and JEG-3 cells transfected with Ad-TP53BP2 or sh-TP53BP2 under hypoxia was detected by western blotting (n= 3). The data are presented as the means ± SDs.**P <0.01,***P <0.001.