Luciferase reporter assay
A luciferase reporter assay was conducted to assess the activity of the
TP53BP2 promoter. For the promoter activity assay, various fragments of
the TP53BP2 promoter (-35/+1, -599/+1, -1018/+1, -1530/+1, -599/+641,
and -2000/+1) were inserted into the pGL3-Basic plasmid. The cells were
then seeded in 24-well plates and cotransfected with the aforementioned
reporter constructs and a Renilla luciferase reporter plasmid. Following
a 48-hour transfection period, the luciferase activities were quantified
via a dual-luciferase reporter assay system (Promega, Madison, USA). The
firefly luciferase activity was determined and normalized to the Renilla
luciferase activity. The reported data represent the average of three
independent experiments.