Statistical analysis
The data from three replicates per experiment are summarized as mean ± SD. The statistical analysis involved one-way ANOVA, followed by the Student-Newman-Keuls’ test for multiple comparisons within treatment groups or Student’s t test for comparisons between two groups. ROC curves and the corresponding area under the curve (AUC) were generated via the R package ”pROC” to assess the diagnostic value of TP53BP2. A significance threshold of P <0.05 was applied.