Rambutan seeds are often discarded as waste, so making it a valuable source to assess their conversion to a commercially available energy alternative. Rambutan seed has fatty acids which have the potential to produce biodiesel. Malwana rambutan seeds were used as biodiesel feedstock in this research. The process carried out using Soxhlet apparatus to extract rambutan seed oil (RSO), then it converted into fatty acid methyl ester (FAME) which is known as biodiesel, by acid esterification followed by base transesterification. Acid value = 0.35mgKOH[[EQUATION]], Kinematic viscosity at 40°C = 2.87[[EQUATION]], Flash point = 60.67°C, Cetane number = 44.21, Distillation temperature = 269.65 °C were the results shown by Malwana rambutan biodiesel, in addition to that GCMS analysis also performed to analyze fatty acid composition of Malwana rambutan seed oil and conformation for the formation of fatty acid methyl ester. From the results, it showed that, the acid value, kinematic viscosity, cetane number and distillation temperature were within the standard whereas falsh point did not meet the standard value. The Malwana rambutan seed oil can be used as raw material for biodiesel production and also can be used as an additive to present diesel according to the obtained results.