Figure 2 GET3B function is involved in the assembly of photosystem (PS) II
(A) Seedlings were germinated and cultivated under etiolating conditions for 5 days on 0% sucrose (top) or 1 % sucrose (bottom) for 5 days before being normally illuminated and subjected to pulse amplitude modulation (PAM) measurements. The Fv/Fm ratio (maximum quantum yield) was determined after 1, 7, 24 hours to monitor PS II assembly. (B) Seedlings were grown under normal illumination on 0 % sucrose (top) and 1 % sucrose (bottom). After 5 days these were subjected to PAM measurements. (C) Seedlings were cultivated under normal conditions for 4 days and either subjected to PAM measurements or (D) used for chlorophyll isolation. Statistical analyses for (A) and (B) were performed using the Student’s t-test, for three independent experiments with 40 seedlings per experiment. Statistical analyses for (C) were performed with ANOVA for 15 independent experiments with 40 seedlings per experiment as well as (D) for 3 experiments with 120 seedlings per experiment.