Rosette measurements
For measurements of rosette diameters and chlorophyll content, plants
with specific genotypes were grown for approximately 4 weeks under the
conditions described above. Rosette diameter was determined by measuring
across the rosette at the point where the distance was largest. For
chlorophyll measurements, 120 seedlings were collected for each genotype
or transgenic line. These were pooled and weighed to estimate the
freshweight. Chlorophyll was extracted with 80% acetone in 2.5 mM
HEPES-KOH (pH 7.5) as described previously (Hackett et al., 2017) and
chlorophyll amounts were calculated from absorbances measured on a
spectrophotometer, as described in Wellburn (1994). Pairwise Student’st tests (two-tailed) were used to compare means of rosette
diameter and ANOVA to compare the chlorophyll concentration.