Plasmids and plasmid construction for protein expression
For the overexpression of GST-tagged mature full-length GET3b (GST-Get3b), the corresponding sequence encoding amino acids (aa) 68-411 was cloned into pGex-4T-3 using the BamHI/SalI restriction sites. The length of the chloroplast transit sequence was predicted by the TargetP-2.0 server (https://services.healthtech.dtu.dk/services/TargetP-2.0/).
Constructs for the expression of His-tagged C-terminal regions of ALB3 corresponding to aa 350-462 (Alb3C-His) and ALB4 corresponding to aa 334-499 (Alb4C-His) were described previously (Ackermann et al., 2021).