Figure 2 GET3B function is involved in the assembly of
photosystem (PS) II
(A) Seedlings were germinated and cultivated under etiolating
conditions for 5 days on 0% sucrose (top) or 1 % sucrose (bottom) for
5 days before being normally illuminated and subjected to pulse
amplitude modulation (PAM) measurements. The Fv/Fm ratio (maximum
quantum yield) was determined after 1, 7, 24 hours to monitor PS II
assembly. (B) Seedlings were grown under normal illumination on
0 % sucrose (top) and 1 % sucrose (bottom). After 5 days these were
subjected to PAM measurements. (C) Seedlings were cultivated
under normal conditions for 4 days and either subjected to PAM
measurements or (D) used for chlorophyll isolation. Statistical
analyses for (A) and (B) were performed using the Student’s t-test, for
three independent experiments with 40 seedlings per experiment.
Statistical analyses for (C) were performed with ANOVA for 15
independent experiments with 40 seedlings per experiment as well as (D)
for 3 experiments with 120 seedlings per experiment.