Terminal insertases of the stromal GET pathway
Uwe Bodensohn*1, Beatrix Dünschede2, Chiara Kuhlmann2, Khushbu Kumari,3Roman Ladig5, Christopher Grefen3, Enrico Schleiff1, Donna Fernandez4and Danja Schünemann2
1Institute for Molecular Biosciences, Goethe University Frankfurt, Max von Laue Str. 9, 60438 Frankfurt, Germany
2Molecular Biology of Plant Organelles, Ruhr University Bochum, Universitätsstraße 150, 44780 Bochum, Germany
3Molecular and Cellular Botany, Ruhr University Bochum, Universitätsstraße 150, 44780 Bochum, Germany
4Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, WI 53706-1381, United States of America
5German cancer research center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
*For correspondence: Uwe Bodensohn, Goethe University Frankfurt, Max von Laue Str. 9, N200/3.02; 60438 Frankfurt, Germany
E-Mail: Bodensohn@bio.uni-frankfurt.de
ORCID iD: https://orcid.org/0000-0002-4698-3757
Tel: (+49) 69 798 29283
This study discloses GET3B’s role in chloroplast biogenesis and function. Comparative proteomic analysis of get3b mutant plastids highlight significant proteome alterations. Functional assays show compromised PS II assembly and CO2 assimilation. Genetic analyses indicate get3b and srp54 as mutual potentiators. Physical interaction studies suggest ALB3 and ALB4 as terminal insertases of GET3B. Together, these findings underpin GET3B’s role in coordinating the assembly and maintenance of the photosynthetic machinery.