Rosette measurements
For measurements of rosette diameters and chlorophyll content, plants with specific genotypes were grown for approximately 4 weeks under the conditions described above. Rosette diameter was determined by measuring across the rosette at the point where the distance was largest. For chlorophyll measurements, 120 seedlings were collected for each genotype or transgenic line. These were pooled and weighed to estimate the freshweight. Chlorophyll was extracted with 80% acetone in 2.5 mM HEPES-KOH (pH 7.5) as described previously (Hackett et al., 2017) and chlorophyll amounts were calculated from absorbances measured on a spectrophotometer, as described in Wellburn (1994). Pairwise Student’st tests (two-tailed) were used to compare means of rosette diameter and ANOVA to compare the chlorophyll concentration.