Figure 5 Transgenes encoding wild-type GET3B can complement alb4 srp54 get3b triple mutants, but not transgenes encoding GET3B with a D124N mutation
Plants were grown in 12-h light/12-h dark conditions for 4 weeks. Top row: Images of (A) alb4 srp54 plants, and(B, C) two independent lines of transgenic plants carrying the ProUBI10:GET3B-STREP transgene in an alb4 srp54 get3b background. Bottom row: Images of (D) alb4 srp54 get3b plants, and(E, F) two independent lines of transgenic plants carrying the ProUBI10:GET3B(D124N)-STREP transgene in an alb4 srp54 get3b background. Bars= 1 cm. (G) Rosette diameters of alb4 srp54 plants and three independent lines of alb4 srp54 get3b plants carrying the ProUBI10:GET3B-STREP transgene. (H) Rosette diameters of untransformed (T0) alb4 srp54 get3b plants and four independent lines of alb4 srp54 get3b plants carrying the ProUBI10:GET3B(D124N)-STREP transgene. Data are presented as means + SE (n= 8-10 individual plants in G, n= 3-8 individual plants in H). Asterisks designate significant differences (Student’s t test, *P<0.05, **P<0.01) between the transgenic plants and either alb4 srp54 plants (G) or alb4 srp54 get3b plants (H).