Figure 1 The get3b mutation and the downstream effects on the chloroplast proteome
(A) Gene model of GET3B and the corresponding T-DNA insertion line analyzed in this study. (B) Genotyping via PCR of get3b . The locations of the used primers are indicated in A as arrowheads. (C) Semi RT-PCR to validate the missing transcript.(D) Western blot analysis of total protein lysates. Wild-type and get3b plastids were isolated and subjected to LC-MS/MS followed by label free quantification. (E) Statistical analysis of proteinaceous hits that were significantly effected in get3b chloroplasts (see Supplemental table S1 for more details). The significantly depleted (F) and significantly enriched(G) proteinaceous hits were categorized according to their cellular function. The color coding in E corresponds to those in F and G.