Figure 6 Expression of transgenes encoding GET3B with a D124N mutation leads to reductions in plant size in alb4 or stic2 genetic backgrounds
Plants were grown in 12-h light/12-h dark conditions for 4 weeks. Top row: Images of (A) Columbia wild-type, (B) alb4 , and (C) stic2 plants. Bottom row: Transgenic plants carrying the (D) ProUBI10:GET3B-STREP transgene in a stic2 background, (E) ProUBI10:GET3B(D124N)-STREP transgene in an alb4 background and(F) ProUBI10:GET3B(D124N)-STREP transgene in a stic2 background. Bars= 1 cm. (G) Rosette diameters of untransformed (T0) stic2 plants and two independent lines of transgenicstic2 plants carrying the ProUBI10:GET3B-STREP transgene.(H) Rosette diameters of untransformed (T0) alb4 (left) orstic2 (right) plants and two independent lines each of transgenicalb4 or stic2 plants carrying the ProUBI10:GET3B(D124N)-STREP transgene. Data are presented as means + SE (n= 10-14 individual plants in G, n= 10-15 individual plants in H). Asterisks indicate significant differences (Student’s t test, *P<0.05, **P<0.01) between the transgenic plants and untransformed (T0) plants of the same genotype.